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Time-Dependent Oxidative Stress Responses of Submerged Macrophyte Vallisneria natans Seedlings Exposed to Ammonia in Combination with Microcystin Under Laboratory Conditions

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Abstract

We studied the antioxidant responses of macrophyte Vallisnerria natans seedlings to combined ammonia (0, 0.21, and 0.85 mg L−1) and microcystin-LR (MC-LR) (0, 10, and 50 μg L−1) for 7 days. Results showed that superoxide dismutase, catalase (CAT), peroxidase, and glutathione were significantly induced by the mixtures of ammonia and MC-LR, and there were significant interactions between ammonia and MC-LR. Specially, CAT increased about fivefold at ammonia 0.85 mg L−1 and MC-LR 50 μg L−1 on day 3. Malondialdehyde fluctuated with both ammonia and MC-LR, and significant interactions were detected between the two stressors. Changes in all the measured variables were time-dependent.

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Acknowledgments

This study was supported by the State Key Fundamental Research and Development Program of China (2008CB418102), Natural Science Foundation of Jiangsu Province (BK2011073).

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We declare that there is no potential.

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Authors and Affiliations

  1. Jiangsu Province Key Laboratory for Biodiversity and Biotechnology, School of Biological Sciences, Nanjing Normal University, 1 Wenyuan Road, Nanjing, 210046, China

    Juan Ge, Jing Zhang & Zhou Yang

  2. Freshwater Fisheries Research Institute of Jiangsu Province, 79 East Cha Ting Street, Nanjing, 210017, China

    Jiajia Li

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  1. Juan Ge
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  2. Jiajia Li
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  3. Jing Zhang
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  4. Zhou Yang
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Correspondence to Zhou Yang.

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Ge, J., Li, J., Zhang, J. et al. Time-Dependent Oxidative Stress Responses of Submerged Macrophyte Vallisneria natans Seedlings Exposed to Ammonia in Combination with Microcystin Under Laboratory Conditions. Bull Environ Contam Toxicol 89, 67–72 (2012). https://doi.org/10.1007/s00128-012-0633-8

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  • DOI: https://doi.org/10.1007/s00128-012-0633-8

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