Abstract
The present study was designed to assess whether supplementation of different levels of vitamin E to long-term arsenic exposed goats affords protection against the oxidative stress caused by the metalloid. Twenty-four crossbred lactating goats were distributed randomly into four groups (control, T1, T2 and T3) of six in each. The animals in T1, T2 and T3 were given 50 mg/kg DM arsenic daily, while in T2 and T3, vitamin E @100 IU and 150 IU/kg DM, respectively, was also supplemented additionally for the period of 12 months. Compared to control, significant (p < 0.05) decline in SOD (45 %), CAT activities of erythrocytes (63 %), plasma total Ig (22 %) and total antioxidant activity (24 %) was observed in only arsenic treated groups and vitamin E supplementation in both doses produced partial mitigation effect against SOD (23 %, 20 %) and CAT (39 %, 48 %) while complete mitigation against total Ig (16 %, 7 %) and antioxidant activity (10 %, 8 %) was found. Average lymphocyte stimulation index at the end of experiment was (p < 0.05) lower in arsenic exposed groups (1.003 ± 0.01) and significant (p < 0.05) recovery was observed in response of vitamin E supplementation at higher doses (1.138 ± 0.03). So, vitamin E is helpful in reducing the burden of arsenic induced oxidative stress and activities of antioxidant enzymes in goats.
Similar content being viewed by others
References
Aebi H (1984) Catalase in vitro. In: Packer L (ed) Methods in enzymology, vol 105. Academic press, New York, pp 121–126
Benzie EFI, Strain JJ (1999) Ferric reducing antioxidant power assay: direct measurement of total antioxidant activity of biological fluids and modified version for simultaneous measurement of total antioxidant and ascorbic acid concentration. Methods Enzymol 299:15–27
Biswas U, Sarkar S, Bhowmik MK, Roy S (1998) Clinicopathological profile of induced chronic arsenic toxicity in goats. Indian J Anim Sci 68:320–323
Biswas U, Sarkar S, Bhowmik MK, Samanta SK, Biswas S (2000) Chronic toxicity of arsenic in goats: clinicobiochemical changes, pathomorphology and tissue residues. Small Rumin Res 38:229–235
Bueter GR (1993) The pecking order of free radicals and antioxidants. Lipid peroxidation, α-tocopherol and ascorbate. Arch Biochem Biophys 300:535–543
Garcia-Shavez E, Jimenez I, Segura B, Razo LMD (2006) Lipid peroxidative damage and distribution of inorganic and its metabolite in the rat nervous system after arsenite exposure: influence of alpha tocopherol. Neurotoxicol 27:1024–1031
Irshad M, Chaudhuri PS (2002) Oxidant-antioxidant system: role and significance in human body. Indian J Exp Biol 40:1233–1239
Madesh M, Balasubramanian KA (1998) Microtiter plate assay for superoxide dismutase using MTT reduction by superoxide. Indian J Biochem Biophys 35:184–188
McEwan AD, Fisher EW (1970) A turbidity test for the estimation of immunoglobulins levels in neonatal calf serum. Clin Chim Acta 17:155
Morand-Fehr P (1981) Nutrition and feeding of goats: application to temperate climate. In: Gall G (ed) Goat production. Academic Press, London, pp 193–233
Meng ZQ, Meng NY (2000) Effects of arsenic on blast transformation and DNA synthesis of human blood lymphocytes. Chemosphere 41(1–2):115–119
Mosmann T (1983) Rapid colorimetric assay for cellular growth and survival application to proliferation and cytotoxicity assay. J Immunol Methods Des 65:55–63
Nandi D, Patra RC, Swarup D (2006) Oxidative stress indices and plasma biochemical parameters during oral exposure to arsenic in rats. Food Chem Toxicol 44:1579–1584
Nandi D, Patra RC, Ranjan R, Swarup D (2008) Role of co-administration of antioxidants in prevention of oxidative injury following sub-chronic exposure to arsenic in rats. Veterinarski Arhiv 78:113–121
NRC (1981) Nutrient requirements of goats: Angora, dairy and meat goats in temperate and tropical countries. National Academy Press, Washington
Pi J, Yamauchi H, Kumagai Y (2002) Evidence for induction of oxidative stress caused by chronic exposure of Chinese residents to arsenic contained in drinking water. Environ Health Persp 110:331–336
Politis I, Hidiroglou M, Batra TR, Gilmore JR, Gorewit RC, Scherf H (1995) Effects of vitamin E on immune function of diary cows. Am J Vet Res 56:179–184
Rana T, Bera AK, Das S, Bhattacharya D, Bandyopadhyay S, Pan D, Das SK (2010) Effect of chronic intake of arsenic-contaminated water on blood oxidative stress indices in cattle in an arsenic-affected zone. Ecotoxicol Environ Safety 73:1327–1332
Tchounwou PB, Wilson B, Ishaque A (1999) Important considerations in the development of public health advisories for arsenic and arsenic containing compounds in drinking water. Rev Environ Health 14:211–219
Wu MM, Chiou HY, Wang TW, Hsues YM, Wang IH, Chen CJ, Lee TC (2001) Association of blood arsenic levels with increased reactive oxidants and decreased antioxidant capacity in a human population of northeastern Taiwan. Environ Health Perspect 109:1011–1017
Yu HS, Liao WT, Chang KL, Yu GL, Chen GS (2002) Arsenic induces tumor necrosis factor alpha release and tumor necrosis factor receptor 1 signaling in T helper cell apoptosis. J Invest Dermatol 119:812–819
Acknowledgments
Authors would like to express their sincere thanks to the Director of the Institute for providing necessary helps.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Das, T.K., Mani, V., Kaur, H. et al. Effect of Vitamin E Supplementation on Arsenic Induced Oxidative Stress in Goats. Bull Environ Contam Toxicol 89, 61–66 (2012). https://doi.org/10.1007/s00128-012-0620-0
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00128-012-0620-0