Abstract
The semi-isolated heart bioassay was used to evaluate the effect of glycoalkaloids extracted from potato leaves on the heart contractile activity of three beetle species Zophobas atratus, Tenebrio molitor and Leptinotarsa decemlineata. The dose–response curves indicated species specific action of tested substances. Application of glycoalkaloids on the continuously perfused Z. atratus heart inhibited progressively frequency contractions; higher concentrations exerted short and reversible cardiac arrests. In the rest two beetle species tested glycoalkaloids caused no cardiotropic effect. In vivo bioassay with 1 day old Z. atratus pupae showed that the extract induces a negative inotropic effect on the heart.
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Acknowledgments
We gratefully acknowledge Prof. Grzegorz Rosinski for critical review of the manuscript, his support and encouragement. We also thank Dr. Danuta Waligóra for supplying Colorado potato beetles.
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Marciniak, P., Adamski, Z., Bednarz, P. et al. Cardioinhibitory Properties of Potato Glycoalkaloids in Beetles. Bull Environ Contam Toxicol 84, 153–156 (2010). https://doi.org/10.1007/s00128-009-9921-3
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DOI: https://doi.org/10.1007/s00128-009-9921-3