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Determination of Phthalates in Diet and Bedding for Experimental Animals Using Gas Chromatography-Mass Spectrometry

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Abstract

We have developed a gas chromatography–mass spectrometry method to measure five phthalates (dibutyl phthalate, butylbenzyl phthalate, di-2-ethylhexyl phthalate, diisooctyl phthalate, and diisononyl phthalate) in diets and beddings for experimental animals. The recoveries from diets and beddings spiked with five phthalates were 98.8%–148% with coefficients of variation of 0.4%–7.8% for diets and 94.7%–146% with coefficients of variation of 1.0%–5.0% for beddings. We analyzed commercial animal diets and beddings, and found that the levels of phthalates varied from sample to sample; the concentrations of five phthalates were 141–1,410 ng/g for diets and 20.5–7,560 ng/g for beddings.

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Acknowledgments

This study was supported by a grant from the Ministry of Health, Labor, and Welfare, Japan.

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Authors and Affiliations

  1. Department of Toxicology, Aichi Prefectural Institute of Public Health, 7-6 Nagare, Tsuji-machi, Kita-ku, Nagoya, 462-8576, Japan

    Fumio Kondo, Masanao Okumura & Hisao Oka

  2. Department of Analytical Chemistry, Hoshi University, 2-4-41 Ebara, Shinagawa-ku, Tokyo, 142-8501, Japan

    Hiroyuki Nakazawa

  3. Department of Obstetrics and Gynecology, School of Medicine, Tokai University, Bohseidai, Isehara, 25-1193, Japan

    Shun-ichiro Izumi & Tsunehisa Makino

  4. Department of Pharmacology, School of Medicine, Aichi Medical University, Nagakute, Aichi, 480-1195, Japan

    Fumio Kondo

Authors
  1. Fumio Kondo
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  2. Masanao Okumura
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  3. Hisao Oka
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  4. Hiroyuki Nakazawa
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  5. Shun-ichiro Izumi
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  6. Tsunehisa Makino
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Correspondence to Fumio Kondo.

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Kondo, F., Okumura, M., Oka, H. et al. Determination of Phthalates in Diet and Bedding for Experimental Animals Using Gas Chromatography-Mass Spectrometry. Bull Environ Contam Toxicol 84, 212–216 (2010). https://doi.org/10.1007/s00128-009-9919-x

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  • DOI: https://doi.org/10.1007/s00128-009-9919-x

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