Abstract
A series of six 96-h static bioassays were performed to validate the use of anchoveta (Engraulis ringens) embryos as test organisms for ecotoxicological studies. The standardization protocol utilized potassium dichromate (K2Cr2O7) as a reference toxicant and egg mortality as the endpoint. The results indicated that the mean sensitivity of anchoveta embryos to potassium dichromate was 156.1 mg L−1 (range: 131–185 mg L−1). The statistical data analysis showed high homogeneity in LC50 values among bioassays (variation coefficient = 11.02%). These results demonstrated that the protocol and handling procedures implemented for the anchoveta embryo bioassays comply with international standards for intra-laboratory precision. After secondary treatment, an effluent from a modern Kraft pulp mill was tested for E. ringens embryo toxicity, finding no significant differences from the controls.
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Acknowledgments
This research was funded by the Programa de Investigación Marina de Excelencia (PIMEX-Nueva Aldea), Universidad de Concepción – CFI-Nueva Aldea. We also thank S. Soto, A. Yañez, M.C. Krautz, J. Méndez, and M.I. Muñoz from the Laboratorio de Oceanografía Pesquera y Ecologia Larval (LOPEL) for their help with sampling at sea and during egg sorting in the laboratory. Filtered seawater was provided by Dr. E. Tarifeño’s research group at the invertebrate hatchery in the Dichato Marine Station of the Universidad de Concepción.
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Llanos-Rivera, A., Castro, L.R., Silva, J. et al. A New Developmental Toxicity Test for Pelagic Fish Using Anchoveta (Engraulis ringens J.). Bull Environ Contam Toxicol 83, 23–28 (2009). https://doi.org/10.1007/s00128-009-9714-8
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DOI: https://doi.org/10.1007/s00128-009-9714-8