Abstract
Aims/hypothesis
Little is known about the subcellular distribution of lipids in insulin-resistant skeletal muscle. However, it has recently been suggested that lipid accumulation in the subsarcolemmal region directly contributes to insulin resistance. Therefore we hypothesised that regional differences in lipid distribution in insulin-resistant muscle may be mediated by: (1) a reduction in fatty acid trafficking into mitochondria; and/or (2) a regional increase in the enzymes regulating lipid synthesis.
Methods
Transmission electron microscopy was used to quantify lipid droplet and mitochondrial abundance in the subsarcolemmal and intermyofibrillar compartments in red and white muscles from lean and obese Zucker rats. To estimate rates of lipid trafficking into mitochondria, the metabolic fate of radiolabelled palmitate was determined. Key enzymes of triacylglycerol synthesis were also determined in each subcellular region.
Results
Subsarcolemmal-compartmentalised lipids represented a small absolute fraction of the overall lipid content in muscle, as regardless of fibre composition (red/white) or phenotype (lean/obese), lipid droplets were more prevalent in the intermyofibrillar region, whereas insulin-resistant white muscles were devoid of subsarcolemmal-compartmentalised lipid droplets. While, in obese animals, lipid droplets accumulated in both subcellular regions, in red muscle of these animals lipids only appeared to be trafficked away from intermyofibrillar mitochondria, a process that cannot be explained by regional differences in the abundance of triacylglycerol esterification enzymes.
Conclusions/interpretation
Lipid accumulation in the subsarcolemmal region is not necessary for insulin resistance. In the intermyofibrillar compartment, the diversion of lipids away from mitochondria in insulin-resistant animals probably contributes to lipid accumulation in this subcellular area.
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Introduction
Insulin resistance is associated with increased circulating lipids, as well as ectopic lipid accumulation in skeletal muscle [1, 2]. In this tissue, intramuscular lipid accumulation seems to occur in two distinct intracellular compartments, namely the subsarcolemmal region located directly beneath the sarcolemmal membrane and in an intermyofibrillar region between the myofibrils [3]. These compartments also harbour subsarcolemmal and intermyofibrillar mitochondria, and therefore intramuscular lipid depots are found in close proximity to mitochondria [4]. Recently it has been suggested that the subcellular distribution of intramuscular lipid droplets in the subsarcolemmal region, rather than the total content of various lipid species, contributes to attenuation of insulin signalling in muscle [5]. There are several potential mechanisms that could result in subcellular variations in intramuscular lipid accretion, including regionally selective impairments of mitochondrial fatty acid oxidation and/or increased abundance of the enzymes involved in triacylglycerol esterification.
Subsarcolemmal and intermyofibrillar mitochondria differ in several characteristics, including size, enzymatic activities and rates of fatty acid oxidation [6–10]. In addition, rates of substrate utilisation in these mitochondrial subpopulations respond differently to various physiological perturbations, including aerobic training-induced adaptations [11], mitochondrial biogenesis induced by peroxisome proliferator-activated receptor (PPAR) co-activator 1α (PGC-1α) [12], pharmacological activation of PPAR [13] and obesity [9]. In Zucker diabetic fatty rats, a model of severe type 2 diabetes, subsarcolemmal, but not intermyofibrillar mitochondrial number, width and density were increased by 50% to 70%, although fatty acid oxidation rates were increased to similar degrees in subsarcolemmal and intermyofibrillar mitochondria [14]. Thus, differences in subsarcolemmal and intermyofibrillar mitochondrial morphology and fatty acid metabolism may be associated with differences in lipid accumulation in proximity to subsarcolemmal and intermyofibrillar mitochondria, particularly in insulin-resistant muscle in which fatty acid transport into muscle is known to be upregulated [9, 15].
On the other hand, lipid accumulation in specific subcellular compartments in muscle may be associated with regional increases in enzymes regulating the rate of triacylglycerol synthesis. Intramuscular lipid droplets are visible via transmission electron microscopy (TEM) and are thought to largely reflect triacylglycerol content [14, 16]. Triacylglycerol esterification can be regulated by a number of enzymes, which in most cell types reside on the endoplasmic reticulum, with the exception of glycerol-3-phosphate acyltransferase 1 (GPAT1), which is located on mitochondria [17]. This raises the possibility that partitioning of fatty acyl-CoA to storage or oxidation can occur in close proximity to the mitochondria, a possibility also implied by the intimate spatial relationship between mitochondria and lipid droplets [5, 14, 18, 19]. However, it is currently unknown whether: (1) the enzymes involved in triacylglycerol synthesis are present and differentially associated in subsarcolemmal and/or intermyofibrillar mitochondrial compartments in skeletal muscle; and (2) whether the subcellular presence of these enzymes is altered in muscles from insulin-resistant animals.
Together, this amounts to tentative evidence that differences in subsarcolemmal and intermyofibrillar mitochondrial fatty acid oxidation and differences in subsarcolemmal and intermyofibrillar lipid accumulation are associated. However, in general, little is known about: (1) the possibility of divergent lipid accumulation patterns in subcellular regions in either highly oxidative (red) and glycolytic (white) muscles with inherently different capacities for lipid metabolism; (2) whether these patterns of lipid accretion are altered in obesity; and (3) whether different lipid accumulation patterns in skeletal muscle are associated with insulin resistance in this tissue. Therefore, in the present study, we examined the accumulation of intramuscular lipids in proximity to subsarcolemmal and intermyofibrillar mitochondrial regions in red and white skeletal muscle of lean and obese Zucker rats, a well-known animal model of obesity and insulin resistance. We also examined, in intact muscle, the levels of radiolabelled palmitate accumulation in subsarcolemmal and intermyofibrillar mitochondria. Our results show that in obese animals excess lipids accumulate primarily in the intermyofibrillar regions of skeletal muscle, as lipid droplet size and density are greatly increased. At the same time, incoming fatty acids are trafficked away from intermyofibrillar mitochondria.
Methods
Animals
Female lean (246 ± 8 g) and obese (400 ± 8 g) Zucker rats aged 10 weeks (Charles River, Baie d’Urfé, QC, Canada) were housed in a climate control facility at the University of Guelph, and allowed free access to rat chow and water. All experiments were performed using procedures approved by the University of Guelph Animal Care Committee.
Basal and insulin-stimulated glucose uptake
Basal and insulin-stimulated 3-O-methylglucose uptake was measured using a submaximal concentration of insulin (150 μU/ml) in perfused rat hindlimb muscles as we have described previously [12, 20].
Biochemical determination of intramuscular lipid content
Intramuscular levels of triacylglycerol, diacylglycerol and ceramide were measured as described previously [21].
Transmission electron microscopy
Sample preparation, sectioning and TEM imaging were performed as we have described previously [14, 16]. Following tissue preparation, samples were embedded in resin (London Resin Company, London, UK) and 100 nm longitudinal sections were cut, mounted on 200 mesh formvar/carbon copper grids (CanEMco, Lakefield, PQ, Canada), and stained with 2% uranyl acetate (wt/vol.) and Reynold’s lead citrate. A minimum of three sections was laid on to each grid (range 3–5) and labelled with serial numbering such that individuals quantifying images were blinded to the phenotype of the animals. Images were obtained from each animal in a randomised systematic order from multiple fibres, including several images acquired at a magnification of ×46,000, ×10,500, ×5,800 or ×3,400 using a CM 10 TEM (Phillips, Hillsboro, OR, USA) at 80 kV and a digital charge-coupled device (CCD) camera (Olympus/SIS Morada, Richmond Hill, ON, Canada).
Quantification of TEM images
We generated 477 images, which were quantified by three investigators. Inter- and intra-investigator variability tests showed no signs of bias and low coefficients of variation (<10%). All images were analysed for mitochondrial variables as we have described previously [14, 16]. We analysed lipid droplet density in two ways, counting (1) the number of lipid droplets in subsarcolemmal and intermyofibrillar regions normalised to muscle area and (2) the relative percentage of subsarcolemmal lipids. The latter was calculated by determining subsarcolemmal lipids normalised to muscle fibre surface area and intermyofibrillar lipids normalised to the volume beneath the surface area, assuming an average radius of 40 μm and cylindrical shape of the fibres as reported by others [5]. The cross-sectional area of individual lipid droplets was determined using a software package (iTEM; Olympus/SIS).
Mitochondrial DNA
Mitochondrial DNA content was determined in the red and white portions of the tibialis anterior muscle using real-time PCR as we have previously reported [12, 14].
Whole-muscle palmitate metabolism
Rates of palmitate esterification and oxidation were determined in soleus muscles as previously described [22].
Palmitate trafficking to mitochondria
Soleus muscle strips were prepared as outlined previously [22]. Muscles were incubated for 60 min in the presence of 37 kBq [3H]palmitate (GE Healthcare, Baie D’Urfe, PQ, Canada) and 150 μU/ml insulin. Thereafter, mitochondria were isolated as previously published [23–25], protein contents were determined using a BCA assay and 100 μl of the radiolabelled mitochondrial suspension was counted using scintillation counting. Counts were normalised to protein content. Preliminary time course experiments showed that lipid accretion was linear for the duration of the 60 min incubation.
Western blotting
Western blotting was performed as previously described [23–25]. Antibodies used were: GPAT1 (Abcam, Cambridge, MA, USA), diacylglycerol acyltransferase (DGAT1) (Abcam), lipin 1 (Abcam), adipose triacylglycerol lipase (Cayman Chemical, Ann Arbor, MI, USA) and hormone-sensitive lipase (Cell Signaling Technology, Danvers, MA, USA).
Statistical analysis
Data were analysed using two-tailed unpaired t tests or 2-way ANOVA, and when appropriate Fisher’s least significant difference post hoc tests. A value of p < 0.05 was considered significant. All data are reported as mean±SEM.
Results
Skeletal muscle insulin resistance
Independent of phenotype, basal and insulin-stimulated rates of glucose transport were greater in red than in white muscles (Fig. 1a, b). However, in obese animals, the basal rates of glucose transport were reduced by ~30% in both muscle types (Fig. 1a). In addition, insulin sensitivity was reduced by ~75% in red and white muscles of the obese animals (Fig. 1b).
Intramuscular lipids
Intramuscular lipid content was determined in two ways. Traditional biochemical analyses were used to quantify intramuscular triacylglycerol, diacylglycerol and ceramide content. In addition, we used TEM images to determine lipid droplet accumulation in selected subcellular compartments.
Biochemical determinations
Regardless of muscle fibre composition, triacylglycerol (red ~3.7-fold, white ~4.2-fold) and diacylglycerol (red 37%, white 85%) contents were increased in obese animals (Fig. 2a, b). Ceramide content was increased in red (39%), but not in white muscles of the obese animals (Fig. 2c).
Lipid droplet size, number and subcellular distribution
TEM images clearly showed that visible intramuscular lipid droplets: (1) were distributed in specific subcellular muscle compartments in proximity to subsarcolemmal and intermyofibrillar mitochondria; (2) differed substantially in lean and obese animals; and (3) displayed the expected muscle distribution (i.e. greater in red than white) (Fig. 3 a–f). Lipid droplets in lean and obese white muscle were not prominent (Fig. 3f). Thus, the comparisons between red and white muscles below are necessarily based on the relatively few regions of white muscle where lipid droplets were observed.
Individual lipid droplet size and number
In all muscles examined, regardless of muscle and phenotype, lipid droplets were more prevalent (approximately four- to fivefold) in the intermyofibrillar than in the subsarcolemmal region (Fig. 3a–f). In lean and obese animals there were also considerable differences in lipid droplets between red and white muscles, as well as between respective muscles within these animals (Fig. 3a–f).
Relative to red muscles in lean animals, the red muscle of obese animals contained considerably more individual lipid droplets per unit area of muscle, both in the subsarcolemmal (approximately fourfold) and the intermyofibrillar (approximately threefold) mitochondrial regions (Fig. 3d). Moreover, whereas the size of individual lipid droplets was increased approximately threefold in the subsarcolemmal and intermyofibrillar regions of obese animals (Fig. 3c), the number of lipid droplets in the latter region was approximately fivefold greater than in the subsarcolemmal region (Fig. 3d). Thus when normalised to fibre surface area, subsarcolemmal lipids only represented a small fraction of the overall lipids in red muscle (lean 5.4 ± 0.5%, obese 9.6 ± 0.5%).
In white muscle, in contrast, lipid droplets were barely present in the subsarcolemmal compartments of lean or obese animals (Fig. 3e, f). The numbers of lipid droplets in the intermyofibrillar region were comparable in lean and obese animals (Fig. 3f). In white muscle, therefore, the increase in total triacylglycerol content (Fig. 2b) appears to be due exclusively to increased droplet size in the limited number of lipid droplets in the intermyofibrillar region (Fig. 3e).
Mitochondrial size and density
Since altered mitochondrial structure and lipid metabolism have been implicated in the development of insulin resistance, we next examined the size, number and density of subsarcolemmal and intermyofibrillar mitochondria in longitudinal sections of red and white muscles from lean and obese animals (Fig. 4a–d).
Mitochondrial size
In general, the qualitative appearance of mitochondria in lean and obese animals did not differ with respect to cristae density. In red muscle, there was no difference in size of individual subsarcolemmal mitochondria in lean and obese animals. In contrast, intermyofibrillar mitochondria were larger (by 58%) in the obese animals (Fig. 4a).
In white muscle, mitochondria were smaller (by ~50%) and less prevalent (Fig. 4c) than in red muscle, regardless of phenotype. In white muscle, moreover, subsarcolemmal mitochondria were rarely observed, while intermyofibrillar mitochondria appeared highly organised in doublets (Fig. 4c) flanking the Z-lines, as has been seen by others [26]. Despite the scarcity of white muscle subsarcolemmal and intermyofibrillar mitochondria, these, when detected, did not differ in size in the two subcellular regions of lean or obese animals (Fig. 4b).
Mitochondrial density in red muscle
In obese animals, red muscle mitochondrial density was increased in the subsarcolemmal (by 21%) and intermyofibrillar (by 27%) mitochondrial regions (Fig. 4d), suggesting an increase in mitochondrial content. This is supported by an increase in red muscle mDNA (Fig. 4e). Given the paucity of subsarcolemmal and intermyofibrillar mitochondria and their diffuse nature in white muscle, we did not quantify mitochondrial density in the two regions of this tissue. However, whole muscle mDNA was increased by 26% in white muscle of obese animals (lean 63 ± 3 vs obese 79 ± 8 arbitrary units), suggesting that, similarly to red muscle, mitochondrial content was increased in this animal group.
Metabolic trafficking of palmitate
As lipid droplets were found, without exception, to be in direct contact with mitochondria (Fig. 5a), we next examined the metabolic fate of palmitate in order to determine the potential cause of lipid accumulation in the obese animals. Palmitate trafficking experiments were not performed in white muscle, as lipid droplets were only prevalent in the red muscle.
Palmitate oxidation and triacylglycerol esterification
In the obese animals, whole-muscle palmitate oxidation was reduced by ~20% (Fig. 5b), while rates of triacylglycerol esterification were increased (by ~20%) (Fig. 5c). Combined, these data suggest that fatty acids are trafficked into lipid droplets.
To further examine this, we determined fatty acid accumulation in the subsarcolemmal and the intermyofibrillar mitochondrial compartments following [3H]palmitate exposure (60 min) in an incubated soleus muscle preparation. In obese animals, the accumulation of radiolabelled fatty acids in subsarcolemmal mitochondria was unaltered (Fig. 5d). In contrast, fatty acid accumulation in intermyofibrillar mitochondria was reduced by 44% (Fig. 5d).
Triacylglycerol esterification enzyme content and subcellular distribution
Given the apparent trafficking of lipids away from intermyofibrillar mitochondria, we next examined whether the enzymes involved in fatty acid esterification were present and increased specifically in intermyofibrillar mitochondria in obese animals, thereby directing fatty acids towards triacylglycerol synthesis and away from mitochondrial oxidation. However, despite the functional differences in the metabolic fate of palmitate in obese animals, there were no differences in the total cellular content of selected enzymes involved in triacylglycerol metabolism (GPAT1, lipin 1 and DGAT1) (Fig. 6a–c). In addition, while GPAT1 was detected in isolated subsarcolemmal and intermyofibrillar mitochondria, GPAT1 content was not altered with obesity in either region. In contrast, lipin 1 and DGAT1 were not detected in isolated mitochondria (Fig. 6b, c).
Discussion
In the current study, we examined the subcellular distribution of lipids in red and white muscles of lean and insulin-resistant animals. Our data demonstrate that: (1) lipid droplets are more prevalent in the intermyofibrillar region in lean and obese animals; (2) insulin-resistant white muscles are devoid of subsarcolemmal lipid droplets and therefore (3) subsarcolemmal lipids are not required for insulin resistance; and (4) within the intermyofibrillar region, lipids are trafficked away from mitochondria in obese animals, a process that (5) cannot be explained by increased abundance of triacylglycerol esterification enzymes. This diversion of lipids away from intermyofibrillar mitochondria probably contributes to lipid accumulation in this subcellular compartment in insulin-resistant muscle.
Total cellular lipids and insulin resistance
Skeletal muscle is key for glucose disposal and is known to take up ~80% of a glucose load [27]. An increase in the intramuscular content of selected lipids has been associated with skeletal muscle insulin resistance [28–30]. Our findings here support this notion, as total intramuscular triacylglycerol and diacylglycerol contents were increased in the red and white muscles examined in the obese animals. In addition, red, but not white, skeletal muscle from obese animals displayed increased ceramide content. It is currently believed that the accumulation of bioactive lipids, such as diacylglycerol [31] and ceramides [32], contributes to the development of insulin resistance [31, 32]. In the current study, the white muscle of obese animals was insulin-resistant, which coincided with increased diacylglycerol, but unaltered ceramide content. Combined, these data suggest that increased ceramide content is not necessarily a pre-requisite for insulin resistance, supporting instead the belief that diacylglycerol content may have a direct role in this condition.
While it was originally thought that the accumulation of triacylglycerol was a direct cause of insulin resistance [30], the view now is that triacylglycerol is a neutral and beneficial lipid storage depot, and that the accumulation of triacylglycerol in insulin resistance skeletal muscle is likely to be a marker of altered lipid homeostasis, rather than a cause of insulin resistance. This view is supported by a number of observations, including the observation that overabundance of DGAT1 increases intramuscular triacylglycerol content, while protecting against diet-induced insulin resistance [33], as well as the finding that exercise-trained athletes display high intramuscular triacylglycerol content, but are highly insulin-sensitive [34, 35]. However, a recent report by Nielsen and colleagues [5] suggests that triacylglycerol lipid droplets can negatively affect insulin signalling if accumulated in the subsarcolemmal subcellular region, thus renewing interest in triacylglycerol accumulation as a potential cause of insulin resistance.
Subcellular distribution of lipids
Regardless of fibre composition or genotype, we found that lipid droplets were larger and more prevalent in the intermyofibrillar region. Moreover, our calculation of the relative abundance of subsarcolemmal lipids, based on assumptions of average fibre diameter and shape in red muscle, suggested that subsarcolemmal lipids only represented approximately 6% to 10% of the overall lipids in the muscle. Lipid droplet content in the intermyofibrillar region increased to a greater absolute magnitude in the muscle fibre of obese Zucker rats. However, given that subsarcolemmal lipids are virtually absent in lean animals, subsarcolemmal lipids increased to a greater relative amount with obesity. Combined, these data imply that lipid droplet accumulation in various subcellular regions in obese animals reflects alterations in total cellular lipid homeostasis. While it is possible that estimates based on fibre diameter and shape underestimated the relative content of subsarcolemmal lipids in the cell, lipid droplets were not detectable in the subsarcolemmal region of white muscle even when this was insulin-resistant, suggesting that lipid droplets in the subsarcolemmal region are not required for the development of insulin resistance. This is in contrast to a recent study by Nielsen et al. [5], who found that increased lipid droplet volume was exclusively limited to the subsarcolemmal compartment in patients with type 2 diabetes. The discrepancy between our study and that of Nielsen and colleagues may be related to species and/or condition. However, it should be noted that subsarcolemmal lipids also did not correlate with insulin sensitivity following aerobic training in the same report by Nielsen et al. [5], with five participants who displayed pronounced reductions in subsarcolemmal lipids having only modest changes in insulin sensitivity and one participant who had increased subsarcolemmal lipids with modestly improved insulin sensitivity [5]. Therefore, based on our observations, it appears that lipid accumulation in the subsarcolemmal region is not a direct cause and/or requirement for the development of insulin resistance in muscle of obese rodents. Presumably, and similarly to biochemical determinations of intramuscular lipids, lipid droplet accumulation in the subsarcolemmal region probably reflects alterations in cellular lipid homeostasis, rather than being a direct cause of insulin resistance.
Mitochondrial subcellular alterations and fatty acid metabolism
A decrease in mitochondrial fatty acid oxidation has been proposed to contribute to lipid accumulation [8, 36]. Therefore regional differences in mitochondrial content and/or function may help to explain subcellular differences in lipid droplets. In the current study, muscle mDNA was increased in muscles of obese animals, which probably accounted for the increases in subsarcolemmal and intermyofibrillar mitochondrial density. Previously, moreover, we had shown that fatty acid oxidation in isolated subsarcolemmal and intermyofibrillar mitochondria remained unaltered (intermyofibrillar) or was increased (subsarcolemmal) [9] in the same animal model. The increase in subsarcolemmal mitochondrial density and fatty acid oxidation may represent compensatory mechanisms designed to prevent lipid droplet accumulation in the subsarcolemmal region. Therefore, reduced mitochondrial fatty acid oxidation apparently cannot account for the observed intramuscular lipid accumulation, a notion that is consistent with contemporary mitochondrial literature [37–41].
However, this literature is based on in vitro measurements that are optimised to determine the capacity of mitochondrial function and therefore may not represent the in vivo oxidative flux. In support of this, in the obese Zucker rat, despite increased mitochondrial density as shown here and elsewhere [9, 38], and despite increased in vitro mitochondrial function [9], we (present study) and others [41] found that rates of total muscle fatty acid oxidation were decreased, while, as found here, rates of triacylglycerol esterification were increased. It should be noted that the notion that fatty acid oxidation is reduced in obese Zucker rats [15, 42, 43] is controversial. Nevertheless, the discrepancy between mitochondrial oxidative capacity and in vivo oxidative flux is echoed in the Zucker diabetic fatty rat, a model of type 2 diabetes where mitochondrial content and isolated mitochondrial function are increased [14], yet in vivo oxidative capacity, as assessed by 31P magnetic resonance spectroscopy, is unaltered [44]. Several mechanisms may explain these data, including the observation that levels of malonyl-CoA, a biological inhibitor of carnitine palmitoyl-transferase I and mitochondrial fatty acid oxidation, are increased in obesity and in individuals with type 2 diabetes [45]. Combined, these data suggest the possibility that fewer fatty acids are transported into mitochondria in obese insulin-resistant muscle, a phenomenon that could contribute to lipid accumulation.
Intramyocellular fatty acid trafficking
To ascertain whether lipids are trafficked away from mitochondria in the obese animal, we incubated muscle in the presence of [3H]palmitate and subsequently isolated subsarcolemmal and intermyofibrillar mitochondria. The tibialis anterior muscle is not appropriate for this in vitro analysis, and so we used the soleus muscle, another oxidative muscle that also displays insulin resistance and lipid accumulation in these animals [46], for these studies. The results with this approach suggest that fewer lipids are transported into intermyofibrillar mitochondria in the in vivo situation. This finding is somewhat surprising, given the now well-recognised observation that rates of fatty acid transport in the obese Zucker rat are substantially increased [9, 15, 41]. Nevertheless, it appears that less [3H]palmitate was transported into intermyofibrillar mitochondria, despite a greater delivery of fatty acids into the muscle’s interior, suggesting that lipids were trafficked away from the mitochondria. The observed phenomenon was unlikely to have been caused by decreased transport capacity into mitochondria, as we have previously shown that carnitine palmitoyl-transferase I activity and rates of mitochondrial fatty acid oxidation are not reduced in these mitochondria [9]. Combined, these data suggest that lipids were trafficked away from the intermyofibrillar mitochondria in an unknown manner.
Given the proximity of intermyofibrillar mitochondria to the majority of lipid droplets in the obese animals, we hypothesised that triacylglycerol esterification enzymes would be increased in the intermyofibrillar region, providing a mechanism for the apparent stealing of lipids away from mitochondrial oxidation. However, similarly to previous reports [47, 48], increases in whole-muscle content of several esterification enzymes were not observed. In addition, lipin-1 and DGAT1 were not detectable on intermyofibrillar mitochondria, while GPAT1 content was unaltered with obesity. We cannot rule out the possibility that levels of these enzymes were increased specifically on the endoplasmic reticulum in the intermyofibrillar region. In addition, adipose tissue triacylglycerol lipase protein was unaltered (lean 100 ± 4, obese 89 ± 4 arbitrary units), while hormone-sensitive lipase cellular protein was increased (lean 100 ± 6, obese 138 ± 16 arbitrary units), suggesting that decreased lipolysis cannot account for the observed trafficking of lipids into lipid droplets. Alternatively, the observation that fewer lipids are transferred into intermyofibrillar mitochondria may simply reflect a situation where mitochondria are already oversupplied with lipids [49]. Nevertheless, as far as we can determine, ours is the first attempt to directly show that fatty acid transport into mitochondria is decreased in insulin-resistant muscle, possibly providing a potential mechanism for lipid accumulation in the intermyofibrillar region.
Summary
In the current study, we determined the subcellular distribution of lipids in insulin-resistant skeletal muscle. While others have suggested that subsarcolemmal lipids contribute to attenuations in insulin signalling [5], the current data suggest that subsarcolemmal lipids are not required for the development of insulin resistance, as insulin-resistant white muscles were devoid of lipids in the subsarcolemmal region. Therefore, similarly to increases in total triacylglycerol content, lipid droplet accumulation in the subsarcolemmal region is likely to simply reflect alterations in total cellular lipid homeostasis. In addition, we provide evidence that in insulin-resistant muscle fatty acids are trafficked away from intermyofibrillar mitochondria and probably into lipid droplets in the intermyofibrillar region. While the underlying mechanism for this diversion of fatty acids remains unknown, we have provided evidence that this is not a direct effect of increased triacylglycerol esterification enzyme content on intermyofibrillar mitochondria.
Abbreviations
- DGAT1:
-
Diacylglycerol acyltransferase 1
- GPAT1:
-
Glycerol-3-phosphate acyltransferase 1
- PPAR:
-
Peroxisome proliferator-activated receptor
- TEM:
-
Transmission electron microscopy
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Acknowledgements
This work was funded by the Natural Sciences and Engineering Research Council of Canada (NSERC; to G.P. Holloway and A. Bonen). A. Bonen is the Canada Research Chair in Metabolism and Health. Equipment was purchased with assistance from NSERC (to A. Bonen) and the Canada Foundation for Innovation (to G.P. Holloway).
Contribution statement
JL performed experiments, interpreted data and wrote the paper. LS, XXH and AC performed experiments, interpreted data and edited the manuscript. AB designed experiments, interpreted data and edited the manuscript. GPH designed and performed experiments, interpreted data and edited the manuscript. All authors have approved the final version of the manuscript.
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The authors declare that there is no duality of interest associated with this manuscript.
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Lally, J.S.V., Snook, L.A., Han, X.X. et al. Subcellular lipid droplet distribution in red and white muscles in the obese Zucker rat. Diabetologia 55, 479–488 (2012). https://doi.org/10.1007/s00125-011-2367-2
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DOI: https://doi.org/10.1007/s00125-011-2367-2