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Development of novel wheat-rye 6RS small fragment translocation lines with powdery mildew resistance and physical mapping of the resistance gene PmW6RS

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Abstract

Key message

Novel wheat-rye 6RS small fragment translocation lines with powdery mildew resistance were developed, and the resistance gene PmW6RS was physically mapped onto 6RS-0.58–0.66-bin corresponding to 18.38 Mb in Weining rye.

Abstract

Rye (Secale cereale L., RR) contains valuable genes for wheat improvement. However, most of the rye resistance genes have not been successfully used in wheat cultivars. Identification of new rye resistance genes and transfer of these genes to wheat by developing small fragment translocation lines will make these genes more usable for wheat breeding. In this study, a broad-spectrum powdery mildew resistance gene PmW6RS was localized on rye chromosome arm 6RS using a new set of wheat-rye disomic and telosomic addition lines. To further study and use PmW6RS, 164 wheat-rye 6RS translocation lines were developed by 60Coγ-ray irradiation. Seedling and adult stage powdery mildew resistance analysis showed that 106 of the translocation lines were resistant. A physical map of 6RS was constructed using the 6RS translocation and deletion lines, and PmW6RS was localized in the 6RS-0.58–0.66-bin, flanked by markers X6RS-3 and X6RS-10 corresponding to the physical interval of 50.23–68.61 Mb in Weining rye genome. A total of 23 resistance-related genes were annotated. Nine markers co-segregate with the 6RS-0.58–0.66-bin, which can be used to rapidly trace the 6RS fragment carrying PmW6RS. Small fragment translocation lines with powdery mildew resistance were backcrossed with wheat cultivars, and 39 agronomically acceptable homozygous 6RS small fragment translocation lines were obtained. In conclusion, this study not only provides novel gene source and germplasms for wheat resistance breeding, but also laid a solid foundation for cloning of PmW6RS.

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The authors declare that all data supporting the findings of this study are available within the article and its supplementary information files.

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Acknowledgements

We would like to thank Pro. Fangpu Han, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China, for providing seed samples of 843, PI 613196, and the set of wheat-rye disomic and telosomic addition lines. We also thank Pro. Dengcai Liu and Dr. Ming Hao from the Triticeae Research Institute, Sichuan Agricultural University, Ya’an, China, for Pm56 seed sample LM47-6.

Funding

This research was supported by the National Key Research and Development Program of China (2021YFD1200600), the National Natural Science Foundation of China (31801358), and the Natural Science Foundation of Hebei Province (C2019503064).

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Authors and Affiliations

  1. Center for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Shijiazhuang, 050021, China

    Jing Wang, Guohao Han, Hong Liu, Hanwen Yan, Yuli Jin, Lijun Cao & Diaoguo An

  2. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China

    Yilin Zhou

  3. Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing, 100101, China

    Diaoguo An

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  1. Jing Wang
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Contributions

DGA conceived the research. JW, GHH, HL, HWY, LJC, and YLZ performed experiments, conducted fieldwork, and performed phenotype observation. JW wrote the manuscript. DGA supervised and revised the manuscript. YLJ also revised the manuscript. All authors read and approved the final manuscript.

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Wang, J., Han, G., Liu, H. et al. Development of novel wheat-rye 6RS small fragment translocation lines with powdery mildew resistance and physical mapping of the resistance gene PmW6RS. Theor Appl Genet 136, 179 (2023). https://doi.org/10.1007/s00122-023-04433-8

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