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Haplotype variants of the stripe rust resistance gene Yr28 in Aegilops tauschii

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Abstract

Key message

Stripe rust resistance gene YrAet672 from Aegilops tauschii accession CPI110672 encodes a nucleotide-binding and leucine-rich repeat domain containing protein similar to YrAS2388 and both these members were haplotypes of Yr28.

Abstract

New sources of host resistance are required to counter the continued emergence of new pathotypes of the wheat stripe rust pathogen Puccinia striiformis Westend. f. sp. tritici Erikss. (Pst). Here, we show that CPI110672, an Aegilops tauschii accession from Turkmenistan, carries a single Pst resistance gene, YrAet672, that is effective against multiple Pst pathotypes, including the four predominant Pst lineages present in Australia. The YRAet672 locus was fine mapped to the short arm of chromosome 4D, and a nucleotide-binding and leucine-rich repeat gene was identified at the locus. A transgene encoding the YrAet672 genomic sequence, but lacking a copy of a duplicated sequence present in the 3′ UTR, was transformed into wheat cultivar Fielder and Avocet S. This transgene conferred a weak resistance response, suggesting that the duplicated 3′ UTR region was essential for function. Subsequent analyses demonstrated that YrAet672 is the same as two other Pst resistance genes described in Ae. tauschii, namely YrAS2388 and Yr28. They were identified as haplotypes encoding identical protein sequences but are polymorphic in non-translated regions of the gene. Suppression of resistance conferred by YrAet672 and Yr28 in synthetic hexaploid wheat lines (AABBDD) involving Langdon (AABB) as the tetraploid parent was associated with a reduction in transcript accumulation.

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Funding

Funding for this research article are the Australian Government Research Training Program (RTP) Scholarship, University of Queensland Centennial Scholarship, Grains Research and Development Corporation of Australia, Borlaug Global Rust Initiative (BGRI) Durable Rust Resistance in Wheat (DRRW) project (administered by Cornell University) and the Biotechnology and Biological Sciences Research Council (BBSRC) Designing Future Wheat Cross-Institute Strategic Programme (BBS/E/J/000PR9780).

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  1. Naveenkumar Athiyannan & Brande B. H. Wulff

    Present address: Biological and Environmental Science and Engineering Division, Center for Desert Agriculture, King Abdullah University of Science and Technology, Thuwal, Saudi Arabia

Authors and Affiliations

  1. Commonwealth Scientific and Industrial Research Organisation Agriculture and Food, Canberra, Australia

    Naveenkumar Athiyannan, Soma Chakraborty, Timothy Hewitt, Dhara Bhatt, Narayana Upadhyaya, Michael Ayliffe, Evans Lagudah & Sambasivam Periyannan

  2. Centre for Crop Science, Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, Brisbane, Australia

    Naveenkumar Athiyannan, Lee T. Hickey & Sambasivam Periyannan

  3. The University of Sydney Plant Breeding Institute, Cobbitty, Australia

    Peng Zhang & Robert McIntosh

  4. AgriBio, LaTrobe R&D Park, Bundoora, Australia

    Kerrie Forrest & Mathew Hayden

  5. Department of Crop Genetics, John Innes Centre, Norwich, UK

    Burkard Steuernagel, Sanu Arora & Brande B. H. Wulff

  6. Campo Experimental Valle de Mexico-INIFAP, Carretera los Reyes-Texcoco, Km 13.5 Coatlinchan, C. P. 56250, Texcoco, Estado de Mexico, Mexico

    Julio Huerta

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  1. Naveenkumar Athiyannan
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  2. Peng Zhang
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Contributions

NA, ESL and SP designed the project. ESL and SP developed the mapping population. NA, PZ, RM, JH, ESL and SP conducted stripe rust phenotyping. NA, SC, KF and MH conducted marker analysis and mapping. BS, SA and BBHW generated the genomic data. NA, TH and NU performed bioinformatics analysis. DB and MA generated the transgenic lines. LH, ESL and SP supervised NA. NA and SP wrote the initial version of the manuscript; all authors provided comments and approved the final version of the manuscript.

Corresponding authors

Correspondence to Evans Lagudah or Sambasivam Periyannan.

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The authors declare no competing interest.

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Communicated by Xiaoquan Qi.

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Athiyannan, N., Zhang, P., McIntosh, R. et al. Haplotype variants of the stripe rust resistance gene Yr28 in Aegilops tauschii. Theor Appl Genet 135, 4327–4336 (2022). https://doi.org/10.1007/s00122-022-04221-w

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  • DOI: https://doi.org/10.1007/s00122-022-04221-w

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