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Establishment and identification of six wheat-Thinopyrum ponticum disomic addition lines derived from partial amphiploid Xiaoyan 7430

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Abstract

Key message

Six wheat-Thinopyrum ponticum disomic addition lines derived from partial amphiploid Xiaoyan 7430 were identified using in situ hybridization and SNP microarray, the homoeologous group and stripe rust resistance of each alien chromosome were determined, and Th. ponticum chromosome-specific markers were developed.

Abstract

Xiaoyan 7430 is a significant partial amphiploid, which is used to set up a bridge for transferring valuable genes from Thinopyrum ponticum (Podp.) Barkworth & D.R. Dewey into common wheat. To accelerate the application of these useful genes in enriching the genetic variability of cultivated wheat by chromosome engineering, a complete set of derived addition lines has been created from Xiaoyan 7430. The chromosome composition of each line was characterized by the combination of genomic in situ hybridization and multicolor fluorescence in situ hybridization (mc-FISH), and the homoeology of each alien chromosome was determined by wheat SNP microarray analysis. Addition line WTA55 with alien group-6 chromosome was evaluated resistant to stripe rust isolates at both the seedling and grain-filling stages (Zadoks scale at z.11 and z.73). Diagnostic marker analysis proved that it could carry a novel stripe rust resistance gene derived from Th. ponticum. Furthermore, a FISH probe and 45 molecular markers specific for alien chromosomes were developed based on specific-locus amplified fragment sequencing (SLAF-seq). Of which 27 markers were separately located on single alien chromosome, and some of them could be used to identify the derived translocation lines. This set of addition lines as well as the molecular markers and the FISH probe will promote the introgression of abundant variation from Th. ponticum into wheat in wheat improvement programs.

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Data and material availability statement

The genotype data and the plant materials reported in this study are available upon request.

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Acknowledgements

We would like to thank Prof. Xueyong Zhang from Chinese Academy of Agricultural Sciences and Prof. Zhijian Chang from Shanxi Academy of Agricultural SciencesAgricultural University for kindly providing seeds of some lines with high resistance to Pst races. This research was supported by the National Natural Science Foundation of China (No. 31971875) and the National Key Research and Development Program of China (2016YFD0102001).

Funding

National Natural Science Foundation of China, 31971875, Qi Zheng, National Key Research and Development Program of China, 2016YFD0102001, Qi Zheng

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Authors and Affiliations

  1. State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing, 100101, China

    Hongwei Jia, Hang Feng, Guotang Yang, Hongwei Li, Bin Li, Zhensheng Li & Qi Zheng

  2. School of Basic Medical Science, Guangxi Medical University, Nanning, 530021, Guangxi Zhuang Autonomous Region, China

    Hongwei Jia & Hang Feng

  3. University of Chinese Academy of Sciences, Beijing, 100049, China

    Guotang Yang

  4. College of Agronomy, Sichuan Agricultural University, Wenjiang, Chengdu, 611130, Sichuan, China

    Shulan Fu

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  1. Hongwei Jia
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Contributions

ZSL and QZ conceived the research; HWJ, HF, GTY and QZ performed the experiments; HWJ and QZ drafted the manuscript; QZ, HWL and BL created the addition lines; SLF conducted the filling-stage resistance evaluation. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Qi Zheng.

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Supplementary Information

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122_2022_4185_MOESM1_ESM.tif

Cytogenetic analysis of Xiaoyan 7430. Th. bessarabicum gDNA (green) was labeled as the probe and Ps. stipifolia and CS gDNAs were used as the block (a). Two probes pAs1 (red) and pSc119.2 (green) were used in mc-FISH (b). Bar = 20 μm (TIF 6061 KB)

122_2022_4185_MOESM2_ESM.tif

PCR amplification results of SSR markers WMC477 and WMC364 and EST-SSR marker X2AS33 in WTA55, its parents and CS. 1: Xiaoyan 7430, 2: WTA55, 3: Xiaoyan 81, 4: CS (TIF 8851 KB)

122_2022_4185_MOESM3_ESM.tif

PCR amplification results of four alien-chromosome specific markers XY7430-35, XY7430-784, XY7430-868 and XY7430-911 in two telosomic and 12 translocation lines derived from Xiaoyan 7430. 1: WTT274, 2: WTT351, 3: WTT73, 4: WTT212, 5: WTT268, 6: WTT269-1, 7: WTT269-2, 8: WTT302, 9: WTT439, 10: X490, 11: X491, 12: X492, 13: WTTe4; 14: WTTe3 (TIF 7767 KB)

122_2022_4185_MOESM4_ESM.tif

Four wheat-Thinopyrum ponticum translocation and one telosomic lines identified by GISH with Th. ponticum gDNA (green) as a probe and FISH with pSc119.2 (green) and pAs1 (red) as probes. a, c, e, g and i are GISH patterns of these lines; b, d, f, h and j are FISH patterns of them. a, b: WTT212, c, d: WTT268, e, f: WTT269-2, g, h: X491, i, j: WTTe4. Bar = 20 μm (TIF 43871 KB)

122_2022_4185_MOESM5_ESM.tif

PCR amplification results of alien chromosome specific markers XY7430-5, XY7430-39, XY7430-899, XY7430-913, XY7430-117 and XY7430-512 in CH7086 and A-3, together with WTA55 and its parent lines. (TIF 11216 KB)

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Jia, H., Feng, H., Yang, G. et al. Establishment and identification of six wheat-Thinopyrum ponticum disomic addition lines derived from partial amphiploid Xiaoyan 7430. Theor Appl Genet 135, 3277–3291 (2022). https://doi.org/10.1007/s00122-022-04185-x

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