Abstract
Key message
Spontaneous haploid genome doubling is not associated with undesirable linkage drag effects. The presence of spontaneous doubling genes allows maximum exploitation of variability from the temperate-adapted BS39 population
Abstract
Tropical non-elite maize (Zea mays L.) germplasm, such as BS39, provides a unique opportunity for broadening the genetic base of U.S. Corn Belt germplasm. In vivo doubled haploid (DH) technology has been used to efficiently exploit non-elite germplasm. It can help to purge deleterious recessive alleles. The objectives of this study were to determine the usefulness of BS39-derived inbred lines using both SSD and DH methods, to determine the impact of spontaneous as compared with artificial haploid genome doubling on genetic variance among BS39-derived DH lines, and to identify SNP markers associated with agronomic traits among BS39 inbreds monitored at testcross level. We developed two sets of inbred lines directly from BS39 by DH and SSD methods, named BS39_DH and BS39_SSD. Additionally, two sets were derived from a cross between BS39 and A427 (SHGD donor) by DH and SSD methods, named BS39 × A427_DH and BS39 × A427_SSD, respectively. Grain yield, moisture, plant height, ear height, stalk lodging, and root lodging were measured to estimate genetic parameters. For genome-wide association analysis, inbred lines were genotyped using genotype-by-sequencing and Diversity Array Technology Sequencing (DArTSeq). Some BS39-derived inbred lines performed better than elite germplasm inbreds and all sets showed significant genetic variance. The presence of spontaneous haploid genome doubling genes did not affect performance of inbred lines. Five SNPs were significant and three of them located within genes related to plant development or abiotic stresses. These results demonstrate the potential of BS39 to add novel alleles to temperate elite germplasm.
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Acknowledgements
This work was supported by USDA’s National Institute of Food and Agriculture (grant numbers: IOW04714, IOW05520; IOW05510; IOW05656; NIFA award 2018-51181-28419 and 2020-51300-32180), US. Department of Agriculture, Agricultural Research Service, the Iowa State University Plant Sciences Institute, Iowa State University Crop Bioengineering Center, R.F. Baker Center for Plant Breeding, and the K.J. Frey Chair in Agronomy at Iowa State University. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. USDA is an equal opportunity provider and Employer.
Funding
The funding was provided by USDA (Grant no. IOW04714, IOW5520, IOW05656, IOW05510) Institute of Food and Agriculture (Grant no. 2018–51181-28419) National Institute of Food and Agriculture (Grant no. 2020–51300-32180)
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ALV, UKF, TL design the project and performed the experiments. IGS, ALV, JE, NB, LTZ, LPMP, GLF analyzed the data. IGS, ALV wrote the manuscript. All authors read and approved the final manuscript.
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Key message
Spontaneous haploid genome doubling is not associated with undesirable linkage drag effects. The presence of spontaneous doubling genes allows maximum exploitation of variability from the temperate-adapted BS39 population.
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Communicated by Alain Charcosset.
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Santos, I.G., Verzegnazzi, A.L., Edwards, J. et al. Usefulness of temperate-adapted maize lines developed by doubled haploid and single-seed descent methods. Theor Appl Genet 135, 1829–1841 (2022). https://doi.org/10.1007/s00122-022-04075-2
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DOI: https://doi.org/10.1007/s00122-022-04075-2