Abstract
Key message
The mutations BnA1.CER4 and BnC1.CER4 produce disordered wax crystals types and alter the composition of epidermal wax, causing increased cuticular permeability and sclerotium resistance.
Abstract
The aerial surfaces of land plants are coated with a cuticle, comprised of cutin and wax, which is a hydrophobic barrier for preventing uncontrolled water loss and environmental damage. However, the mechanisms by which cuticle components are formed are still unknown in Brassica napus L. and were therefore assessed here. BnA1.CER4 and BnC1.CER4, encoding fatty acyl-coenzyme A reductases localizing to the endoplasmic reticulum and highly expressed in leaves, were identified and functionally characterized. Expression of BnA1.CER4 and BnC1.CER4 cDNA in yeast (Saccharomyces cerevisiae) induced the accumulation of primary alcohols with chain lengths of 26 carbons. The mutant line Nilla glossy2 exhibited reduced wax crystal types, and wax composition analysis showed that the levels of branched primary alcohols were decreased, whereas those of the other branched components were increased. Further analysis showed that the mutant had reduced water retention but enhanced resistance to Sclerotinia sclerotiorum. Collectively, our study reports that BnA1.CER4 and BnC1.CER4 are fatty acyl-coenzyme A reductase genes in B. napus with a preference for branched substrates that participate in the biosynthesis of anteiso-primary alcohols.
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Acknowledgments
We would like to thank Xianpeng Yang (Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden) for providing the yeast expression vector. We also thank Jinhua Nan (College of Plant Science and Technology, Huazhong Agricultural University) for providing the wax analysis method.
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This work was supported by the National Key Research and Development of China (2017YFD0102004) and the Fundamental Research Funds for the Central Universities (2662016PY063)
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JL acquired, analyzed, and interpreted most of the data and was a major contributor in writing the manuscript. BY designed the work. LX Z participated in the development of molecular markers and contributed to language modification. BQ W and HD W participated in the planting and collection of plant materials. SQ Z and IM contributed to language modification. JW, CZ M, CD, JX T, JX S, and TD F provided instruments, equipment, and technical support. All authors approved the final manuscript.
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Communicated by Maria Laura Federico.
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Liu, J., Zhu, L., Wang, B. et al. BnA1.CER4 and BnC1.CER4 are redundantly involved in branched primary alcohols in the cuticle wax of Brassica napus. Theor Appl Genet 134, 3051–3067 (2021). https://doi.org/10.1007/s00122-021-03879-y
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DOI: https://doi.org/10.1007/s00122-021-03879-y