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Introgression and pyramiding of genetic loci from wild Brassica oleracea into B. napus for improving Sclerotinia resistance of rapeseed

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Abstract

Key message

Resistant rapeseed lines pyramided with multiple resistant QTLs derived from Brassica oleracea were developed via a hexaploidy strategy.

Abstract

Rapeseed (Brassica napus L.) suffers heavily from Sclerotinia stem rot, but the breeding of Sclerotinia-resistant rapeseed cultivar has been unsuccessful. During the study, interspecific hexaploids were generated between rapeseed variety ‘Zhongshuang 9’ and a wild B. oleracea which was highly resistant to S. sclerotiorum, followed by backcrossing with Zhongshuang 9 and successive selfing. By molecular marker-assisted selection, three major resistant QTLs were transferred and pyramided from B. oleracea into two BC1F8 lines which exhibited ~ 35% higher resistance level than Zhongshuang 9 and produced good seed yield and seed quality. It is the first report on successful development of Sclerotinia-resistant rapeseed lines by introducing multiple resistant loci from wild B. oleracea. This study revealed the effectiveness of pyramiding multiple QTLs in improving Sclerotinia resistance in rapeseed and provided a novel breeding strategy on utilization of B. oleracea in rapeseed improvement.

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Acknowledgements

This study was funded by the National Key R&D Program of China (2018YFD0100500 and 2018YFE0108000), the National Nature Science Foundation of China (31971978), the Project of Chongqing Science and Technology Commission (cstc2019jcyj-zdxm0012), the Fundamental Research Funds for the Central Universities (XDJK2018AA004) and Chongqing Research Program of Basic Research and Frontier Technology (cstc2017jcyjAX0107).

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Author notes

  1. Jiaqin Mei and Chaoguo Shao have contributed equally to this work.

Authors and Affiliations

  1. College of Agronomy and Biotechnology, Southwest University, Chongqing, 400716, China

    Jiaqin Mei, Chaoguo Shao, Ruhan Yang, Yuxia Feng, Yang Gao, Yijuan Ding, Jiana Li & Wei Qian

  2. Academy of Agricultural Sciences, Southwest University, Chongqing, 400716, China

    Jiaqin Mei, Chaoguo Shao, Ruhan Yang, Yuxia Feng, Yang Gao, Yijuan Ding, Jiana Li & Wei Qian

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  1. Jiaqin Mei
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  2. Chaoguo Shao
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  3. Ruhan Yang
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  4. Yuxia Feng
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  5. Yang Gao
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  6. Yijuan Ding
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  7. Jiana Li
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  8. Wei Qian
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Contributions

JM and CS directed the project and wrote the manuscript, RY and YF conducted the resistance evaluation, YG and YD performed the marker-assisted selection and the field work, and JL and WQ designed the experiment.

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Correspondence to Wei Qian.

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Mei, J., Shao, C., Yang, R. et al. Introgression and pyramiding of genetic loci from wild Brassica oleracea into B. napus for improving Sclerotinia resistance of rapeseed. Theor Appl Genet 133, 1313–1319 (2020). https://doi.org/10.1007/s00122-020-03552-w

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  • DOI: https://doi.org/10.1007/s00122-020-03552-w

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