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Development and molecular cytogenetic identification of a new wheat-rye 4R chromosome disomic addition line with resistances to powdery mildew, stripe rust and sharp eyespot

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Abstract

Key message

A wheat-rye 4R chromosome disomic addition line with resistances to powdery mildew, stripe rust, sharp eyespot and high kernel number per spike was developed and characterized by molecular cytogenetic method as novel resistant germplasm.

Abstract

Rye (Secale cereale L.), a close relative of common wheat, is an important and valuable gene donor with multiple disease resistance for wheat improvement. However, resistance genes derived from rye have successively lost resistance to pathogens due to the coevolution of pathogen virulence and host resistance. Development and identification of new effective resistance gene sources from rye therefore are of special importance and urgency. In the present study, a wheat-rye line WR35 was produced through distant hybridization, embryo rescue culture, chromosome doubling and backcrossing. WR35 was then proven to be a new wheat-rye 4R disomic addition line using sequential GISH (genomic in situ hybridization), mc-FISH (multicolor fluorescence in situ hybridization) and ND-FISH (non-denaturing FISH) with multiple probes, mc-GISH (multicolor GISH), rye chromosome arm-specific marker analysis and SLAF-seq (specific-locus amplified fragment sequencing) analysis. At the adult stage, WR35 exhibited high levels of resistance to the powdery mildew (Blumeria graminis f. sp. tritici, Bgt) and stripe rust (Puccinia striiformis f. sp. tritici, Pst) pathogens prevalent in China, and a highly virulent isolate of Rhizoctonia cerealis, the cause of wheat sharp eyespot. At the seedling stage, it was highly resistant to 22 of 23 Bgt isolates and four Pst races. Based on its disease responses to different pathogen isolates, WR35 may possess resistance gene(s) for powdery mildew, stripe rust and sharp eyespot, which differed from the known resistance genes from rye. In addition, WR35 was cytologically stable and produced high kernel number per spike. Therefore, WR35 with multi-disease resistances and desirable agronomic traits should serve as a promising bridging parent for wheat chromosome engineering breeding.

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Abbreviations

Bgt :

Blumeria graminis f. sp. tritici

CS:

Chinese Spring wheat

DAPI:

4,6-Diamidino-2-phenylindole

EST-SSR:

Expressed sequence tag-simple sequence repeat

FISH:

Fluorescence in situ hybridization

GISH:

Genomic in situ hybridization

IT:

Infection type

mc-FISH:

Multicolor FISH

ND-FISH:

Non-denaturing FISH

SLAF:

Specific-locus amplified fragment sequencing

PCR:

Polymerase chain reaction

Pst :

Puccinia striiformis f. sp. tritici

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Acknowledgements

The authors thank Dr. Yilin Zhou and Dr. Shichang Xu from the State Key Laboratory for Biology of Plant Disease and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China, and Dr. Shibin Cai from Institute of Food Crops, Jiangsu Academy of Agricultural Science, Nanjing, China, for conducting assessment of the reactions to powdery mildew, stripe rust and sharp eyespot. This research was supported by the National Key Research and Development Program of China (Nos. 2016YFD0102002 and 2016YFD0100102) and the National Natural Science Foundation of China (No. 31771793).

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Authors and Affiliations

  1. Center for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Shijiazhuang, China

    Diaoguo An, Pengtao Ma, Guohao Han, Jing Wang, Yunfeng Xu, Yuli Jin & Xiaotian Zhang

  2. The State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China

    Qi Zheng, Fangpu Han & Qiaoling Luo

  3. The National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, China

    Lihui Li

  4. Province Key Laboratory of Plant Breeding and Genetics, Sichuan Agriculture University, Chengdu, Sichuan, China

    Shulan Fu

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  1. Diaoguo An
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An, D., Ma, P., Zheng, Q. et al. Development and molecular cytogenetic identification of a new wheat-rye 4R chromosome disomic addition line with resistances to powdery mildew, stripe rust and sharp eyespot. Theor Appl Genet 132, 257–272 (2019). https://doi.org/10.1007/s00122-018-3214-3

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