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QTL mapping of carrot resistance to leaf blight with connected populations: stability across years and consequences for breeding

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An Erratum to this article was published on 18 May 2016

Abstract

Key message

Combining biparental and multiparental connected population analyses was useful for the identification of 11 QTLs in two new genetic backgrounds of carrot resistance to Alternaria dauci and for breeding recommendations.

Abstract

Leaf blight due to the fungus Alternaria dauci is the major carrot foliar disease worldwide. Some resistance QTLs have been previously identified in one population, but the evaluation of additional genetic backgrounds with higher level of resistance would give opportunities for breeders to combine them by pyramiding. For this purpose, two segregating populations were evaluated twice across 4 years in the same environment (1) to compare the efficiency of the single vs. the connected populations approach for characterizing the new sources of carrot resistance to Alternaria dauci; (2) to evaluate the stability of QTLs over the years; and (3) to give recommendations to breeders for marker-assisted selection. Single and connected analyses were complementary; their combination allowed the detection of 11 QTLs. Connected analyses allowed the identification of common and specific QTLs among the two populations and the most favorable allele at each QTL. Important contrasts between allelic effects were observed with four and five most favorable alleles coming from the two resistant parental lines, whereas two other favorable alleles came from the susceptible parental line. While four QTLs were consistent across years, seven were detected within a single year. The heritabilities for both populations PC2 and PC3 were high (75 and 78 %, respectively), suggesting that the resistance of carrot to A. dauci was little affected by these environmental conditions, but the instability of QTL over years may be due to changing environmental conditions. The complementarity between these parental lines in terms of interesting allelic combinations is also discussed.

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Acknowledgments

We are grateful to our colleagues from INEM (Remi Gardet and Jacky Granger, Agrocampus Ouest-IRHS, Angers) involved in tunnel experiments, and our colleagues involved in field testing in the Landes region [B. Peyre, L. Oger and M. Lovera (Vilmorin & Cie); P. Berthy and C. Poisblaud (HMClause)], Stéphan Plas (Invénio) for providing meteorological data and finding field for evaluations in 2014, Cédric Loi (Vilmorin & Cie, Chappes) for adjusting values, Sylvain Jasson and Brigitte Mangin (INRA, UBIA, Toulouse) for their helpful advice and interesting discussion on QTL detection with MCQTL, Laurent Crespel for his valuable contribution to the writing of the manuscript and Gail Wagman and Peter Balint-Kurti for reviewing the English. This work was financed by the French General Board for Companies (‘Création Variétale Potagère’ FCE project, 2007–2010) and by the Permanent Technical Committee of Selection (CTPS, Ministry of Agriculture).

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Correspondence to V. Le Clerc.

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Communicated by M. J. Havey.

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Le Clerc, V., Marques, S., Suel, A. et al. QTL mapping of carrot resistance to leaf blight with connected populations: stability across years and consequences for breeding. Theor Appl Genet 128, 2177–2187 (2015). https://doi.org/10.1007/s00122-015-2576-z

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