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A near-isogenic line (NIL) collection in diploid strawberry and its use in the genetic analysis of morphologic, phenotypic and nutritional characters

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First near-isogenic line collection in diploid strawberry, a tool for morphologic, phenotypic and nutritional QTL analysis.

Abstract

Diploid strawberry (Fragaria vesca), with a small genome, has a high degree of synteny with the octoploid cultivated strawberry (F. × ananassa), so can be used as a simplified model for genetic analysis of the octoploid species. Agronomically interesting traits are usually inherited quantitatively and they need to be studied in large segregating progenies well characterized with molecular markers. Near-isogenic lines (NILs) are tools to dissect quantitative characters and identify some of their components as Mendelian traits. NILs are fixed homozygous lines that share the same genetic background from a recurrent parent with a single introgression region from a donor parent. Here, we developed the first NIL collection in Fragaria, with F. vesca cv. Reine des Vallées as the recurrent parent and F. bucharica as the donor parent. A collection of 39 NILs was identified using a set of single sequence repeat markers. The NILs had an average introgression of 32 cM (6 % of genome) and were phenotyped over several years in two locations. This collection segregates for agronomic characters, such as flowering, germination, fruit size and shape, and nutritional content. At least 16 QTLs for morphological and reproductive traits, such as round fruits and vegetative propagation, and seven for nutritional traits such as sugar composition and total polyphenol content, were identified. The NIL collection of F. vesca can significantly facilitate understanding of the genetics of many traits and provide insight into the more complex F. × ananassa genome.

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Acknowledgments

The authors thank J. Ribes and A. Ortigosa for technical assistance. This work was funded by Grants RTA2007-00063 and AGL2010-21414 from the Spanish Ministry of Science. MU was supported by a fellowship FPI from the Spanish Ministry of Education and JB was supported by a fellowship FI from the Generalitat de Catalunya.

Conflict of interest

The authors declare that they have no conflict of interest.

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  1. Julio Bonet

    Present address: Plant Response Biotech S.L. Centro de Empresas, Parque Científico y Tecnológico Montegancedo, 28223, Pozuelo de Alarcón, Madrid, Spain

Authors and Affiliations

  1. IRTA, Center for Research in Agricultural Genomics (CSIC-IRTA-UAB-UB), Campus UAB, 08193, Bellaterra, Barcelona, Spain

    María Urrutia, Julio Bonet, Pere Arús & Amparo Monfort

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  1. María Urrutia
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Correspondence to Amparo Monfort.

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Communicated by A. H. Schulman.

M. Urrutia and J. Bonet contributed equally to this work.

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122_2015_2503_MOESM1_ESM.png

Sup. Fig. 1 Fragaria map. Markers in red colour were developed in this paper. Nomenclature and description of other markers are as in previously published maps (Ruiz-Rojas et al. 2010; Sargent et al. 2011) (PNG 2820 kb)

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Urrutia, M., Bonet, J., Arús, P. et al. A near-isogenic line (NIL) collection in diploid strawberry and its use in the genetic analysis of morphologic, phenotypic and nutritional characters. Theor Appl Genet 128, 1261–1275 (2015). https://doi.org/10.1007/s00122-015-2503-3

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