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Mutations in chicory FEH genes are statistically associated with enhanced resistance to post-harvest inulin depolymerization

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Nucleotidic polymorphisms were identified in fructan exohydrolases genes which are statistically associated with enhanced susceptibility to post-harvest inulin depolymerization.

Abstract

Industrial chicory (Cichorium intybus L.) root is the main commercial source of inulin, a linear fructose polymer used as dietary fiber. Post-harvest, inulin is depolymerized into fructose which drastically increases processing cost. To identify genetic variations associated with enhanced susceptibility to post-harvest inulin depolymerization and related free sugars content increase, we used a candidate-gene approach focused on inulin and sucrose synthesis and degradation genes, all members of the family 32 of glycoside hydrolases (GH32). Polymorphism in these genes was first investigated by carrying out EcoTILLING on two groups of chicory breeding lines exhibiting contrasted response to post-harvest inulin depolymerization. This allowed the identification of polymorphisms significantly associated with depolymerization in three fructan exohydrolase genes (FEH). This association was confirmed on a wider panel of 116 unrelated families in which the FEH polymorphism explained 35 % of the post-harvest variance for inulin content, 36 % of variance for sucrose content, 18 % for inulin degree of polymerization, 23 % for free fructose content and 22 % for free glucose content. These polymorphisms were associated with significant post-harvest changes of inulin content, inulin chain length and free sugars content.

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Acknowledgments

The authors acknowledge the Walloon Region (DGARNE-Belgium) for supporting this research (Grant D31-1221).

Conflict of interest

O. M. and C. N. are members of Cosucra-Group Warcoing S.A.

Ethical standards

The authors acknowledge that the experiments described in this paper comply with the current laws of the country in which they were performed.

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Authors and Affiliations

  1. Research Unit in Plant Biology, University of Namur, 61 rue de Bruxelles, 5000, Namur, Belgium

    Nicolas Dauchot & Pierre Van Cutsem

  2. Earth and Life Institute, Université catholique de Louvain, Croix du Sud L7.05.11, 1348, Louvain-la-Neuve, Belgium

    Pierre Raulier, Pierre Bertin & Xavier Draye

  3. Chicoline-Cosucra, Rue de la Sucrerie 1, 7740, Warcoing, Belgium

    Olivier Maudoux & Christine Notté

Authors
  1. Nicolas Dauchot
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  2. Pierre Raulier
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  3. Olivier Maudoux
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  4. Christine Notté
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  5. Pierre Bertin
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  6. Xavier Draye
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  7. Pierre Van Cutsem
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Corresponding author

Correspondence to Nicolas Dauchot.

Additional information

Communicated by I. Paran.

Electronic supplementary material

Below is the link to the electronic supplementary material.

ESM_1.pdf: Sequence alignment of 1-FEH I reference cDNA sequence (AJ242538) with two 1-FEH I alleles (partial sequences) isolated from lines susceptible or resistant to post-harvest inulin depolymerization, respectively.

ESM_2.pdf: Sequence alignment of 1-FEH IIa reference cDNA sequence (AJ295033) with two 1-FEH IIa alleles (partial sequences) isolated from lines susceptible or resistant to post-harvest inulin depolymerization, respectively.

ESM_3.pdf: Sequence alignment of 1-FEH IIb reference cDNA sequence (AJ295034) with two 1-FEH IIb alleles (partial sequences) isolated from lines susceptible or resistant to post-harvest inulin depolymerization, respectively.

ESM_4.pdf: Means and standard error of the three different 1-FEH IIa genotypes, among 116 lines, for five carbohydrates-related parameters, before exposure to cold temperatures.

ESM 5.pdf: Schematic representation of the two 1-FEH IIa alleles, respectively, identified in depolymerization-susceptible (DS lines, D+ allele, JQ585639) or depolymerization-resistant (DR lines, d- allele, JQ585638) lines.

Supplementary material 1 (PDF 96 kb)

Supplementary material 2 (PDF 95 kb)

Supplementary material 3 (PDF 93 kb)

Supplementary material 4 (PDF 191 kb)

Supplementary material 5 (PDF 117 kb)

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Dauchot, N., Raulier, P., Maudoux, O. et al. Mutations in chicory FEH genes are statistically associated with enhanced resistance to post-harvest inulin depolymerization. Theor Appl Genet 127, 125–135 (2014). https://doi.org/10.1007/s00122-013-2206-6

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