Skip to main content
SpringerLink
Log in

Genetic mapping of the powdery mildew resistance gene in soybean PI 567301B

  • Original Paper
  • Published:
Theoretical and Applied Genetics Aims and scope Submit manuscript

Abstract

Powdery mildew (PMD) of soybean [Glycine max (L.) Merr.] is caused by the fungus Microsphaera diffusa. Severe infection of PMD on susceptible varieties often causes premature defoliation and chlorosis of the leaves, which can result in considerable yield losses under favorable environmental conditions for disease development in the field. A total of 334 F7-derived recombinant inbred lines (RILs) from a cross of a PMD susceptible soybean cultivar Wyandot and PMD-resistant PI 567301B were used for genetic mapping of PMD resistance in PI 567301B and for development of molecular markers tightly linked to the gene. The result of the PMD screening for each line in the field was in agreement with that in the greenhouse test. The genetic map containing the PMD resistance gene was constructed in a 3.3 cM interval flanked by two simple sequence repeat (SSR) markers on chromosome 16. The PMD resistance gene was mapped at the same location with SSR marker BARCSOYSSR_16_1291, indicating that there was no recombination between the 334 RILs and this marker. In addition, a single nucleotide polymorphism (SNP) marker developed by high-resolution melting curve analysis and a cleaved amplified polymorphic sequence (CAPS) marker with Rsa1 recognition site were used for the genetic mapping. These two markers were also mapped to the same genomic location with the PMD resistance gene. We validated three tightly linked markers to the PMD resistance gene using 38 BC6F2 lines and corresponding BC6F2:3 families. The three marker genotypes of the backcross lines predicted the observed PMD phenotypes of the lines with complete accuracy. We have mapped a putatively novel single dominant PMD resistance gene in PI 567301B and developed three new molecular markers closely linked to the gene. Molecular markers developed from this study may be used for high-throughput marker-assisted breeding for PMD resistance with the gene from PI 567301B.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

References

  • Bachman MS, Tamulonis JP, Nickell CD, Bent AF (2001) Molecular markers linked to brown stem rot resistance genes, Rbs1 and Rbs2, in soybean. Crop Sci 41:527–535

    Article  CAS  Google Scholar 

  • Bai Y, Pavan S, Zheng Z, Zappel NF, Reinstädler A, Lotti C, De Giovanni C, Ricciardi L, Lindhout P, Visser R, Theres K, Panstruga R (2008) Naturally occurring broad-spectrum powdery mildew resistance in a Central American tomato accession is caused by loss of Mlo function. Mol Plant Microbe Interact 21:30–39

    Article  PubMed  CAS  Google Scholar 

  • Buschges R, Hollricher K, Panstruga R, Simons G, Wolter M, Frijters A, van Daelen R, van der Lee T, Diergaarde P, Groenendijk J, Topsch S, Vos P, Salamini F, Schulze-Lefert P (1997) The barley Mlo gene: a novel control element of plant pathogen resistance. Cell 88:695–705

    Article  PubMed  CAS  Google Scholar 

  • Caldwell BE (1966) Inheritance of a strain-specific ineffective nodulation in soybeans. Crop Sci 6:427–428

    Article  Google Scholar 

  • Concibido VC, Denny RL, Boutin SR, Hautea R, Orf JH, Young ND (1994) DNA marker analysis of loci underlying resistance to soybean cyst nematode (Heterodera glycines Ichinohe). Crop Sci 34:240–246

    Article  CAS  Google Scholar 

  • Concibido VC, Lange DA, Denny RL, Orf JH, Young ND (1997) Genome mapping of soybean cyst nematode resistance genes in ‘Peking’, PI 90763, and PI 88788 using DNA markers. Crop Sci 37:258–264

    Article  CAS  Google Scholar 

  • Dunleavy JM (1980) Yield losses in soybeans induced by powdery mildew. Plant Dis 64:291–292

    Article  Google Scholar 

  • Gonçalves ECP, Mauro AOD, Centurion MAPC (2002) Genetics of resistance to powdery mildew (Microsphaera diffusa) in Brazilian soybean populations. Genet Mol Biol 25:339–342

    Article  Google Scholar 

  • Grau C (2006) Powdery Mildew of Soybean. Department of Plant Pathology, Madison, WI. http://www.planthealth.info/pdf_docs/powdery_mildew_WI.pdf. Accessed 14 Dec 2011

  • Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucl Acids Symp Ser 41:95–98

  • Hyten DL, Choi IY, Song Q, Specht JE, Carter TE, Shoemaker RC, Hwang EY, Matukumalli LK, Cregan PB (2010) A high density integrated genetic linkage map of soybean and the development of a 1536 universal soy linkage panel for QTL mapping. Crop Sci 50:960–968

    Article  CAS  Google Scholar 

  • Jun TH, Rouf Mian MA, Michel AP (2012) Genetic mapping revealed two loci for soybean aphid resistance in PI 567301B. Theor Appl Genet 124:13–22

    Article  PubMed  CAS  Google Scholar 

  • Kanazin V, Marek LF, Shoemaker RC (1996) Resistance gene analogs are conserved and clustered in soybean. Proc Natl Acad Sci USA 93:11746–11750

    Article  PubMed  CAS  Google Scholar 

  • Kang ST, Mian MA (2010) Genetic map of the powdery mildew resistance gene in soybean PI 243540. Genome 53:400–405

    Article  PubMed  CAS  Google Scholar 

  • Kilen TC, Hartwig EE, Keeling BL (1974) Inheritance of a second major gene for resistance to phytophthora rot in soybeans. Crop Sci 14:260–262

    Article  Google Scholar 

  • Kim KS, Hill CB, Hartman GL, Hyten DL, Hudson ME, Diers BW (2010) Fine mapping of the soybean aphid-resistance gene Rag2 in soybean PI 200538. Theor Appl Genet 121:599–610

    Article  PubMed  CAS  Google Scholar 

  • Lohnes DG, Bernard RL (1992) Inheritance of resistance to powdery mildew in soybeans. Plant Dis 76:964–965

    Article  Google Scholar 

  • Lohnes DG, Wagner RE, Bernard RL (1993) Soybean genes Rj2, Rmd, and Rps2 in linkage group 19. J Hered 84:109–111

    CAS  Google Scholar 

  • Ma H, Kong Z, Fu B, Li N, Zhang L, Jia H, Ma Z (2011) Identification and mapping of a new powdery mildew resistance gene on chromosome 6D of common wheat. Theor Appl Genet 123:1099–1106

    Article  PubMed  CAS  Google Scholar 

  • Michelmore RW, Paran I, Kesseli RV (1991) Identification of markers linked to disease-resistance genes by bulked segregant analysis: a rapid method to detect markers in specific genomic regions by using segregating populations. Proc Natl Acad Sci USA 88:9828–9832

    Article  PubMed  CAS  Google Scholar 

  • Mignucci JS, Lim SM (1980) Powdery mildew (Microspraera diffusa) development on soybeans with adult-plant resistance. Phytopathology 70:919–921

    Article  Google Scholar 

  • Mulrooney B (2009) Weekly Crop Update: Soybean Disease Update. University of Delaware Cooperative Extension. http://agdev.anr.udel.edu/weeklycropupdate/?p=1550. Accessed 14 Dec 2011

  • Pavan S, Schiavulli A, Appiano M, Marcotrigiano AR, Cillo F, Visser RG, Bai Y, Lotti C, Ricciardi L (2011) Pea powdery mildew er1 resistance is associated to loss-of-function mutations at a MLO homologous locus. Theor Appl Genet 123:1425–1431

    Article  PubMed  Google Scholar 

  • Phillips DV (1984) Stability of Microsphaera dijfusa and the effect of powdery mildew on yield of soybean. Plant Dis 68:953–956

    Google Scholar 

  • Polzin KM, Lohnes DG, Nickell CD, Shoemaker RC (1994) Integration of Rps2, Rmd, and Rj2 into linkage group J of the soybean molecular map. J Hered 85:300–303

    CAS  Google Scholar 

  • Richter TE, Ronald PC (2000) The evolution of disease resistance genes. Plant Mol Biol 42:195–204

    Article  PubMed  CAS  Google Scholar 

  • Saghai-Maroof MA, Soliman KM, Jorgensen RA, Allard RW (1984) Ribosomal DNA spacer-length polymorphisms in barley: mendelian inheritance, chromosomal location, and population dynamics. Proc Natl Acad Sci USA 81:8014–8018

    Article  PubMed  CAS  Google Scholar 

  • SAS Institute (2002) The SAS system for Windows. Release 9.00. SAS Institute Inc., Cary, NC, USA

  • Silva OC, Seganfredo R (1999) Quantificação de danos ocasionados pordoenças de final de ciclo e oídio, em duas variedades de soja. In: CONGRESSO BRASILEIRO DE SOJA, Embrapa Soja, Londrina, Brasil, p 460

  • Song Q, Jia G, Zhu Y, Grant D, Nelson RT, Hwang EY, Hyten DL, Cregan PB (2010) Abundance of SSR motifs and development of candidate polymorphic SSR markers (BARCSOYSSR_1.0) in soybean. Crop Sci 50:1950–1960

    Article  CAS  Google Scholar 

  • Van Ooijen JW (2006) JoinMap 4. Software for the calculation of genetic linkage maps in experimental populations. B. V. Kyazma, Wageningen

    Google Scholar 

  • Vuong TD, Sleper DA, Shannon JG, Nguyen HT (2010) Novel quantitative trait loci for broad-based resistance to soybean cyst nematode (Heterodera glycines Ichinohe) in soybean PI 567516C. Theor Appl Genet 121:1253–1266

    Article  PubMed  CAS  Google Scholar 

  • Wei F, Gobelman-Werner K, Morroll SM, Kurth J, Mao L, Wing R, Leister D, Schulze-Lefert P, Wise RP (1999) The Mla (Powdery Mildew) resistance cluster is associated with three NBS-LRR gene families and suppressed recombination within a 240-kb DNA interval on chromosome 5S (1HS) of barley. Genetics 153:1929–1948

    PubMed  CAS  Google Scholar 

  • Yorinori JT, Hiromoto DM (1998) Determinação de perdas em soja causadas por doenças fúngicas. In: EMBRAPA—Centro Nacional de Pesquisa de Soja, vol 118. Londrina, pp 112–114

  • Yu J, Herrmann M (2006) Inheritance and mapping of a powdery mildew resistance gene introgressed from Avena macrostachya in cultivated oat. Theor Appl Genet 113:429–437

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Entomology, The Ohio State Univ/OARDC, 1680 Madison Avenue, Wooster, OH, 44691, USA

    Tae-Hwan Jun & Andrew P. Michel

  2. Department of Horticulture and Crop Science, USDA-ARS, The Ohio State University, 1680 Madison Avenue, Wooster, OH, 44691, USA

    M. A. Rouf Mian

  3. Department of Crop Science and Biotechnology, Dankook University, Cheonan, 330-714, Korea

    Sung-Taeg Kang

Authors
  1. Tae-Hwan Jun
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. M. A. Rouf Mian
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Sung-Taeg Kang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Andrew P. Michel
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to M. A. Rouf Mian.

Additional information

Communicated by J. Ray.

Trade and manufacturers’ names are necessary to report factually on available data; however, the USDA neither guarantees nor warrants the standard of the product, and the use of the name by the USDA implies no approval of the product to the exclusion of others that may also be suitable.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Jun, TH., Mian, M.A.R., Kang, ST. et al. Genetic mapping of the powdery mildew resistance gene in soybean PI 567301B. Theor Appl Genet 125, 1159–1168 (2012). https://doi.org/10.1007/s00122-012-1902-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00122-012-1902-y

Keywords

Search

Navigation