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The genetic characterisation of stripe rust resistance in the German wheat cultivar Alcedo

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Abstract

Stripe rust resistance in the German winter wheat cv. Alcedo has been described as durable, the resistance having remained effective when grown extensively in Germany and Eastern Europe between 1975 and 1989. Genetic characterisation of field resistance in a cross between Alcedo and the stripe rust susceptible UK winter wheat cv. Brigadier identified two major QTL in Alcedo located on the long arms of chromosomes 2D (QPst.jic-2D) and 4B (QPst.jic-4B). Stripe rust resistance was evaluated by measuring the extent of fungal growth, percentage infection (Pi) and the necrotic/chlorotic response of the plant to infection, infection type (IT). Both QPst.jic-2D and QPst.jic-4B contributed significantly to the reduction in stripe rust infection (Pi), with QPst.jic-2D explaining up to 36.20% and QPst.jic-4B 28.90% of the phenotypic variation measured for Pi. Both QTL were identified by the IT phenotypic scores, with QPst.jic-2D in particular being associated with a strong necrotic phenotype (low IT), QPst.jic-2D explaining up to 53.10% of IT phenotypic variation and QPst.jic-4B 22.30%. In addition, two small effect QTL for field stripe rust resistance were identified in Brigadier, QPst.jic-1B on the long arm of chromosome 1B and QPst.jic-5A on the short arm of chromosome 5A. The influence of QPst.jic-1B was primarily seen with the Pi phenotype, contributing up to 13.10% of the explained phenotypic variation. QPst.jic-5A was only detected using an approximate multiple-QTL model and selecting markers linked to the major effect QTL, QPst.jic-2D and QPst.jic-4B as co-factors. Seedling stripe rust resistance was also mapped in the cross, which confirmed the location of Yr17 from Brigadier to the short arm of chromosome 2A. A seedling expressed QTL was also located in Alcedo that mapped to the same location as the field stripe rust resistance QPst.jic-2D.

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Authors and Affiliations

  1. Department of Disease and Biology, John Innes Centre, Norwich Research Park, Colney Lane, Norwich, NR4 7UH, UK

    L. J. Jagger, R. MacCormack & L. A. Boyd

  2. Limagrain UK Ltd, Station Road, Docking, Norfolk, PE31 8LS, UK

    C. Newell

  3. Limagrain UK Ltd, Windmill Avenue, Woolpit, Bury St. Edmunds, Suffolk, IP30 9UP, UK

    S. T. Berry

Authors
  1. L. J. Jagger
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  2. C. Newell
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  3. S. T. Berry
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  4. R. MacCormack
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  5. L. A. Boyd
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Corresponding author

Correspondence to L. A. Boyd.

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Communicated by X. Xia.

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Jagger, L.J., Newell, C., Berry, S.T. et al. The genetic characterisation of stripe rust resistance in the German wheat cultivar Alcedo. Theor Appl Genet 122, 723–733 (2011). https://doi.org/10.1007/s00122-010-1481-8

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  • DOI: https://doi.org/10.1007/s00122-010-1481-8

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