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Mapping and validation of QTLs for resistance to aphids and whiteflies in melon

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Abstract

Aphis gossypii and Bemisia tabaci are severe hemipteran pests of melon crops and breeding for resistance to both insects is required to reduce pesticide use. Resistance was evaluated for its effect on behaviour and biotic potential of both hemipterans in a population of recombinant inbred lines (RILs) derived from the cross Védrantais × PI 161375. Insect variability was considered using two A. gossypii clones and two B. tabaci populations. Two additive QTLs affected the whiteflies. Four additive QTLs and two couples of epistatic QTLs affected the aphids. Amongst them, a major QTL affects both behaviour and biotic potential of A. gossypii and therefore a same R gene induces both antixenosis and antibiosis. This major QTL colocalizes with the Vat gene belonging to the NBS-LRR gene family. No loci affected both aphids and whiteflies contrary to what was observed for the Mi1.2 gene, a NBS-LRR gene in tomato. Original populations with different allelic compositions at QTLs affecting A. gossypii were built by one inter-crossing of RILs used for the mapping process. The genetic background was shown homogeneous between these populations what allowed validating QTLs and investigating the effect of allelic combinations at QTLs. Effects of QTLs were stronger than expected and some QTLs had a wider spectrum than expected. This strategy of validation appeared rapid and low cost.

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Acknowledgments

We thank Pascale Mistral, Virginie Chareyron and Albert Huc for technical assistance. This work was financially supported by the Région Provence-Alpes-Côte d’Azur-France (contract 2331, project: “Durabilité de la résistance du melon à Aphis gossypii et capacité adaptative de ce puceron”). Sophie Thomas recieved a PhD fellowship funded by INRA and the Région Provence-Alpes-Côte d’Azur, France.

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Authors and Affiliations

  1. Génétique et Amélioration des Fruits et Légumes, INRA, UR1052, B.P. 94 F-84143, Montfavet cedex, France

    Nathalie Boissot, Sophie Thomas & Catherine Dogimont

  2. Biologie et Génétique des Interactions Plantes Parasites CIRAD, INRA, UMR 385, TA A-54/K Campus International de Baillarguet, 34398, Montpellier cedex 5, France

    Nicolas Sauvion

  3. Productions Végétales Domaine Duclos, INRA, UR 979, 97170, Petit-Bourg, French West Indies

    Cécile Marchal & Claudie Pavis

Authors
  1. Nathalie Boissot
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  2. Sophie Thomas
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  3. Nicolas Sauvion
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  4. Cécile Marchal
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  5. Claudie Pavis
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  6. Catherine Dogimont
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Correspondence to Nathalie Boissot.

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Communicated by C. Gebhardt.

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Boissot, N., Thomas, S., Sauvion, N. et al. Mapping and validation of QTLs for resistance to aphids and whiteflies in melon. Theor Appl Genet 121, 9–20 (2010). https://doi.org/10.1007/s00122-010-1287-8

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  • DOI: https://doi.org/10.1007/s00122-010-1287-8

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