Skip to main content
SpringerLink
Log in

Marker-assisted characterization of durum wheat Langdon–Golden Ball disomic substitution lines

  • Original Paper
  • Published:
Theoretical and Applied Genetics Aims and scope Submit manuscript

Abstract

The durum wheat cultivar ‘Golden Ball’ (GB) is a source of resistance to wheat sawfly due to its superior solid stem. In the late 1980s, Dr. Leonard Joppa developed a complete set of 14 ‘Langdon’ (LDN)–GB disomic substitution (DS) lines by using GB as the chromosome donor and LDN as the recipient. However, these substitution lines have not been previously characterized and reported in the literature. The objectives of this study were to confirm the authenticity of the substituted chromosomes and to analyze the genetic background of the 14 LDN–GB DS lines with the aid of molecular markers, and to further use the substitution lines for chromosomal localization of DNA markers and genes conferring the superior stem solidness in GB. Results from simple sequence repeat marker analysis validated the authenticity of the substituted chromosomes in 14 LDN–GB DS lines. Genome-wide scans using the target region amplification polymorphism (TRAP) marker system produced a total of 359 polymorphic fragments that were used to compare the genetic background of substitution lines with that of LDN. Among the polymorphic TRAP markers, 134 (37.3%) and 185 (51.5%) were present in LDN and GB, respectively, with only 10 (2.8%) derived from Chinese Spring. Therefore, marker analysis demonstrated that each LDN–GB DS line had a pair of chromosomes from GB with a genetic background similar to that of LDN. Of the TRAP markers generated in this study, 200 were successfully assigned to specific chromosomes based on their presence or absence in the corresponding LDN–GB DS lines. Also, evaluation of stem solidness in the substitution lines verified the presence of a major gene for stem solidness in chromosome 3B. Results from this research provides useful information for the utilization of GB and LDN–GB DS lines for genetic and genomic studies in tetraploid wheat and for the improvement of stem solidness in both durum and bread wheat.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  • Chao S, Zhang W, Dubcovsky J, Sorrells M (2007) Evaluation of genetic diversity and genome-wide linkage disequilibrium among U.S. wheat (Triticum aestivum L.) germplasm representing different market classes. Crop Sci 47:1018–1030

    Article  CAS  Google Scholar 

  • Chu CG, Xu SS, Friesen TL, Faris JD (2008) Whole genome mapping in a wheat doubled haploid population using SSRs and TRAPs and the identification of QTL for agronomic traits. Mol Breed 22:251–266

    Article  CAS  Google Scholar 

  • Clark JA, Ball CR, Martin JH (1922) Classification of American wheat varieties. Bull 1074 USDA, Washington, DC

  • Clarke FR, Clarke JM, Knox RE (2002) Inheritance of stem solidness in eight durum wheat crosses. Can J Plant Sci 82:661–664

    Google Scholar 

  • Clarke FR, DePauw RM, Aung T (2005) Registration of sawfly resistant hexaploid spring wheat germplasm lines derived from durum. Crop Sci 45:1665–1666

    Article  Google Scholar 

  • Cook JP, Wichman DM, Martin JM, Bruckner PL, Talbert LE (2004) Identification of microsatellite markers associated with a stem solidness locus in wheat. Crop Sci 44:1397–1402

    CAS  Google Scholar 

  • Du C, Hart GE (1998) Triticum turgidum L. 6A and 6B recombinant substitution lines: extended linkage maps and characterization of residual background alien genetic variation. Theor Appl Genet 96:645–653

    Article  CAS  Google Scholar 

  • Eckroth EG, McNeal FH (1953) Association of plant characters in spring wheat with resistance to the wheat stem sawfly. Agron J 45:400–404

    Google Scholar 

  • Engledow FL (1923) The inheritance of glume-length in a wheat cross (continued). J Genet 13:79–100

    Article  Google Scholar 

  • Engledow FL, Hutchinson BA (1926) Inheritance in wheat. II. T. turgidum × T. durum crosses with notes on the inheritance of solidness of straw. J Genet 16:19–23

    Article  Google Scholar 

  • GrainGenes-SQL (2004) Query resources: mapped loci for EST-derived probes. http://www.wheat.pw.usda.gov/cgi-bin/westsql/map_locus.cgi, updated on 17 February 2004, verified on 20 April 2008

  • Harding RM, Boyce AJ, Clegg JB (1992) The evolution of tandemly repetitive DNA: recombination rules. Genetics 132:847–859

    CAS  PubMed  Google Scholar 

  • Hayat MA, Martin JM, Lanning SP, McGuire CF, Talbert LE (1995) Variation for stem solidness and its association with agronomic traits in spring wheat. Can J Plant Sci 75:775–780

    Google Scholar 

  • Houshmand S, Knox RE, Clarke FR, Clarke JM (2007) Microsatellite markers flanking a stem solidness gene on chromosome 3BL in durum wheat. Mol Breed 20:261–270

    Article  CAS  Google Scholar 

  • Hu J, Vick BA (2003) TRAP (target region amplification polymorphism), a novel marker technique for plant genotyping. Plant Mol Biol Rep 21:289–294

    Article  CAS  Google Scholar 

  • Huang XQ, Börner A, Röder MS, Ganal MW (2002) Assessing genetic diversity of wheat (Triticum aestivum L.) germplasm using microsatellite markers. Theor Appl Genet 105:699–707

    Article  CAS  PubMed  Google Scholar 

  • Joppa LR, Cantrell RG (1990) Chromosomal location of genes for grain protein content of wild tetraploid wheat. Crop Sci 30:1059–1064

    Article  CAS  Google Scholar 

  • Joppa LR, Williams ND (1988) Langdon durum disomic substitution lines and aneuploid analysis in tetraploid wheat. Genome 30:222–228

    Article  Google Scholar 

  • Kemp HJ (1934) Studies of solid stem wheat varieties in relation to wheat stem sawfly control. Sci Agric 15:30–38

    Google Scholar 

  • Korzun V, Börner A, Worland AJ, Law CN, Röder MS (1997) Application of microsatellite markers to distinguish inter-varietal chromosome substitution lines of wheat (Triticum aestivum L.). Euphytica 95:149–155

    Article  CAS  Google Scholar 

  • Lanning SP, Fox P, Elser J, Martin JM, Blake NK, Talbert LE (2006) Microsatellite markers associated with a secondary stem solidness locus in wheat. Crop Sci 46:1701–1703

    Article  CAS  Google Scholar 

  • Larson RI, MacDonald MD (1959) Cytogenetics of solid stem in common wheat. II. Stem solidness of monosomic lines of the variety S-615. Can J Bot 37:368–378

    Google Scholar 

  • Larson RI, MacDonald MD (1962) Cytogenetics of solid stem in common wheat. IV. Aneuploid lines of the variety Rescue. Can J Genet Cytol 4:97–104

    Google Scholar 

  • Larson RI, MacDonald MD (1963) Inheritance of the type of solid stem in Golden Ball (Triticum durum). III. The effect of selection for solid stem beyond F5 in hexaploid segregates of the hybrid Rescue (T. aestivum) X Golden Ball. Can J Genet Cytol 5:437–444

    Google Scholar 

  • Lebsock KL, Koch EJ (1968) Variation of stem solidness in wheat. Crop Sci 8:170

    Article  Google Scholar 

  • Li J, Klindworth DL, Shireen F, Cai X, Hu J, Xu SS (2006) Molecular characterization and chromosome-specific TRAP-marker development for Langdon durum D-genome disomic substitution lines. Genome 49:1545–1554

    Article  CAS  PubMed  Google Scholar 

  • Liu ZH, Anderson JA, Hu J, Friesen TL, Rasmussen JB, Faris JD (2005) A wheat intervarietal genetic linkage map based on microsatellite and target region amplified polymorphism markers and its utility for detecting quantitative trait loci. Theor Appl Genet 111:782–794

    Article  CAS  PubMed  Google Scholar 

  • McKenzie H (1965) Inheritance of sawfly reaction and stem solidness in spring wheat crosses: Sawfly reaction. Can J Plant Sci 45:583–589

    Article  Google Scholar 

  • McNeal FH (1961) Segregation for stem solidness in a Triticum aestivum × T. durum wheat cross. Crop Sci 1:111–114

    Article  Google Scholar 

  • McNeal FH, Berg MA (1979) Stem solidness and its relationship to grain yield in 17 spring wheat crosses. Euphytica 28:89–91

    Article  Google Scholar 

  • McNeal FH, Watson CA, Berg MA, Wallace LE (1965) Relationship of stem solidness to yield and lignin content in wheat selections. Agron J 57:20–21

    Article  CAS  Google Scholar 

  • McNeal FH, Wallace LE, Berg MA (1974) Semidwarfness, stem solidness, and tillering of F2 plants from 17 spring wheat crosses. Crop Sci 14:490–492

    Article  Google Scholar 

  • Pestsova E, Salina E, Börner A, Korzun V, Maystrenko OI, Röder MS (2000) Microsatellites confirm the authenticity of inter-varietal chromosome substitution lines of wheat (Triticum aestivum L). Theor Appl Genet 101:95–99

    Article  CAS  Google Scholar 

  • Putnam LG (1942) A study of the inheritance of solid stems in some tetraploid wheats. Sci Agric 22:594–607

    Google Scholar 

  • SAS Institute (2008) SAS/STAT 9.2 User’s Guide. SAS Institute, Inc, Cary, NC

    Google Scholar 

  • Slotta TAB, Brady L, Chao S (2008) High throughput tissue preparation for large scale genotyping experiments. Mol Ecol Resources 8:83–87

    Article  CAS  Google Scholar 

  • Somers DJ, Isaac P, Edwards K (2004) A high-density microsatellite consensus map for bread wheat (Triticum aestivum L.). Theor Appl Genet 109:1105–1114

    Article  CAS  PubMed  Google Scholar 

  • Wallace LE, McNeal FH, Berg MA (1973) Minimum stem solidness in wheat for resistance to the wheat stem sawfly. J Econ Entomol 66:1121–1123

    Google Scholar 

  • Weiss MJ, Morrill WL (1992) Wheat stem sawfly (Hymenoptra Cephidae) revisited. Am Entomol 38:241–245

    Google Scholar 

  • Xu SS, Hu J, Faris JD (2003) Molecular characterization of Langdon durum-Triticum dicoccoides chromosome substitution lines using TRAP (target region amplification polymorphism) markers. In: Pogna NE, Romanò M, Pogna EA, Galterio G (eds) Proceedings of the 10th international wheat genetics symposium, vol. 1, Paestum, Italy, 1–6 September 2003, Istituto Sperimentale per la Cerealicoltura, Rome, Italy, pp 91–94

  • Xu SS, Khan K, Klindworth DL, Faris JD, Nygard G (2004) Chromosomal location of genes for novel glutenin subunits and gliadins in wild emmer wheat (Triticum turgidum L. var. dicoccoides). Theor Appl Genet 108:1221–1228

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

The authors thank Drs. Chao-chien Jan and Zhao Liu for critical review of the manuscript. This research was supported by the USDA-ARS CRIS Project No. 5442-22000-033-00D.

Author information

Authors and Affiliations

  1. Northern Crop Science Laboratory, USDA-ARS, 1307 18th Street North, Fargo, ND, 58105-5677, USA

    Steven S. Xu, S. Chao, D. L. Klindworth & J. D. Faris

  2. Department of Plant Sciences, North Dakota State University, Fargo, ND, 58105, USA

    C. G. Chu & E. M. Elias

Authors
  1. Steven S. Xu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. C. G. Chu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. S. Chao
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. D. L. Klindworth
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. J. D. Faris
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. E. M. Elias
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Steven S. Xu.

Additional information

Communicated by J. Snape.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Xu, S.S., Chu, C.G., Chao, S. et al. Marker-assisted characterization of durum wheat Langdon–Golden Ball disomic substitution lines. Theor Appl Genet 120, 1575–1585 (2010). https://doi.org/10.1007/s00122-010-1277-x

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00122-010-1277-x

Keywords

Search

Navigation