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Development of SSR markers derived from SSR-enriched genomic library of eggplant (Solanum melongena L.)

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Abstract

Eggplant (Solanum melongena L.), also known as aubergine or brinjal, is an important vegetable in many countries. Few useful molecular markers have been reported for eggplant. We constructed simple sequence repeat (SSR)-enriched genomic libraries in order to develop SSR markers, and sequenced more than 14,000 clones. From these sequences, we designed 2,265 primer pairs to flank SSR motifs. We identified 1,054 SSR markers from amplification of 1,399 randomly selected primer pairs. The markers have an average polymorphic information content of 0.27 among eight lines of S. melongena. Of the 1,054 SSR markers, 214 segregated in an intraspecific mapping population. We constructed cDNA libraries from several eggplant tissues and obtained 6,144 expressed sequence tag (EST) sequences. From these sequences, we designed 209 primer pairs, 7 of which segregated in the mapping population. On the basis of the segregation data, we constructed a linkage map, and mapped the 236 segregating markers to 14 linkage groups. The linkage map spans a total length of 959.1 cM, with an average marker distance of 4.3 cM. The markers should be a useful resource for qualitative and quantitative trait mapping and for marker-assisted selection in eggplant breeding.

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Acknowledgments

The authors are grateful to two anonymous referees for many valuable comments, Ms. H. Maeda and Ms. Y. Kitamura for their technical assistance, and field staff in NIVTS NARO for their technical assistance in plant cultivation. This work was supported by a grant from “Development of DNA marker-aided selection technology for plant and animals” funded by the Ministry of Agriculture, Forestry and Fisheries of Japan (DM-2105).

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Authors and Affiliations

  1. National Institute of Vegetable and Tea Science (NIVTS), National Agriculture and Food Research Organization (NARO), 360 Kusawa, Ano, Tsu, Mie, 514-2392, Japan

    Tsukasa Nunome, Satomi Negoro, Koji Miyatake, Hirotaka Yamaguchi, Akio Ohyama & Hiroyuki Fukuoka

  2. Society for Techno-Innovation of Agriculture, Forestry and Fisheries (STAFF), 446-1 Ippaizuka, Kamiyokoba, Tsukuba, Ibaraki, 305-0854, Japan

    Izumi Kono & Hiroyuki Kanamori

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  1. Tsukasa Nunome
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  2. Satomi Negoro
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  3. Izumi Kono
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  4. Hiroyuki Kanamori
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  5. Koji Miyatake
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  6. Hirotaka Yamaguchi
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  7. Akio Ohyama
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  8. Hiroyuki Fukuoka
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Correspondence to Tsukasa Nunome.

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Communicated by R. Waugh.

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Nunome, T., Negoro, S., Kono, I. et al. Development of SSR markers derived from SSR-enriched genomic library of eggplant (Solanum melongena L.). Theor Appl Genet 119, 1143–1153 (2009). https://doi.org/10.1007/s00122-009-1116-0

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  • DOI: https://doi.org/10.1007/s00122-009-1116-0

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