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Grain characterization and milling behaviour of near-isogenic lines differing by hardness

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Abstract

Wheat grain hardness is a major factor affecting the milling behaviour and end-product quality although its exact structural and biochemical basis is still not understood. This study describes the development of new near-isogenic lines selected on hardness. Hard and soft sister lines were characterised by near infrared reflectance (NIR) and particle size index (PSI) hardness index, grain protein content, thousand kernel weight and vitreousness. The milling behaviour of these wheat lines was evaluated on an instrumented micromill which also measures the grinding energy and flour particle size distribution was investigated by laser diffraction. Endosperm mechanical properties were measured using compression tests. Results pointed out the respective effect of hardness and vitreousness on those characteristics. Hardness was shown to influence both the mode of fracture and the mechanical properties of the whole grain and endosperm. Thus, this parameter also acts on milling behaviour. On the other hand, vitreousness was found to mainly play a role on the energy required to break the grain. This study allows us to distinguish between consequences of hardness and vitreousness. Hardness is suggested to influence the adhesion forces between starch granules and protein matrix whereas vitreousness would rather be related to the endosperm microstructure.

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Acknowledgments

We would like to thank M. Ramaroson (ARVALIS - Institut du végétal) and G. Maraval (INRA, Montpellier) for their skilful help in wheat grain milling and C. Olivé (ENSAM, Montpellier) for its technical assistance. This work is part of a French research project between INRA (National Institute of Agronomic Research, Paris) and AFSA (French Association of the Small Grain Cereal Breeders, Paris), ANMF (National Association of French Flour-milling, Paris), ARVALIS-Institut du végétal (Paris), IRTAC (Paris), Danone Vitapole (Palaiseau), Tripette et Renaud–Chopin (Villeneuve la Garenne) and ULICE (Riom).

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Authors and Affiliations

  1. UMR Ingénierie des Agropolymères et Technologies Emergentes, INRA-ENSAM, 2 Place Viala, 34060, Montpellier Cedex 01, France

    V. Greffeuille, J. Abecassis & V. Lullien-Pellerin

  2. ARVALIS-Institut du végétal, 16 rue Nicolas Fortin, 75013, Paris, France

    V. Greffeuille & C. Bar L’Helgouac’h

  3. UMR de Génétique Végétale, INRA INA-PG UPS ferme du Moulon, 91190, Gif sur Yvette, France

    M. Rousset

  4. UMR Amélioration et Santé des Plantes, INRA, 234 avenue du Brézet, 63039 , Clermont-Ferrand Cedex 2, France

    F.-X. Oury & A. Faye

Authors
  1. V. Greffeuille
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  2. J. Abecassis
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  3. M. Rousset
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  4. F.-X. Oury
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  5. A. Faye
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  6. C. Bar L’Helgouac’h
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  7. V. Lullien-Pellerin
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Correspondence to V. Lullien-Pellerin.

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Communicated by M. Morell.

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Greffeuille, V., Abecassis, J., Rousset, M. et al. Grain characterization and milling behaviour of near-isogenic lines differing by hardness. Theor Appl Genet 114, 1–12 (2006). https://doi.org/10.1007/s00122-006-0403-2

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  • DOI: https://doi.org/10.1007/s00122-006-0403-2

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