Skip to main content
SpringerLink
Log in

AB-QTL analysis in spring barley. I. Detection of resistance genes against powdery mildew, leaf rust and scald introgressed from wild barley

  • Original Paper
  • Published:
Theoretical and Applied Genetics Aims and scope Submit manuscript

Abstract

The objective of this study was to map new resistance genes against powdery mildew (Blumeria graminis f. sp. hordei L.), leaf rust (Puccinia hordei L.) and scald [Rhynchosporium secalis (Oud.) J. Davis] in the advanced backcross doubled haploid (BC2DH) population S42 derived from a cross between the spring barley cultivar ‘Scarlett’ and the wild barley accession ISR42-8 (Hordeum vulgare ssp. spontaneum). Using field data of disease severity recorded in eight environments under natural infestation and genotype data of 98 SSR loci, we detected nine QTL for powdery mildew, six QTL for leaf rust resistance and three QTL for scald resistance. The presence of the exotic QTL alleles reduced disease symptoms by a maximum of 51.5, 37.6 and 16.5% for powdery mildew, leaf rust and scald, respectively. Some of the detected QTL may correspond to previously identified qualitative (i.e. Mla) and to quantitative resistance genes. Others may be newly identified resistance genes. For the majority of resistance QTL (61.0%) the wild barley contributed the favourable allele demonstrating the usefulness of wild barley in the quest for resistant cultivars.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1

Similar content being viewed by others

References

  • Abbott DC, Burdon JJ, Jarosz AM, Brown AHD, Mueller WJ, Read BJ (1991) The relationship between seedling infection types and field reactions to leaf scald in Clipper barley backcross lines. Aust J Agric Res 42:801–809

    Article  Google Scholar 

  • Abbott DC, Lagudah ES, Brown AHD (1995) Identification of RFLPs flanking a scald resistance gene of barley chromosome 6. J Heredity 86:152–154

    CAS  Google Scholar 

  • Anonymous (2004) Bundessortenamt, Beschreibende Sortenliste Getreide, Mais, Ölfrüchte, Leguminosen, Hackfrüchte. Deutscher Landwirtschaftsverlag

  • Backes G, Graner A, Foroughi-Wehr B, Fischbeck G, Wenzel G, Jahoor A (1995) Localization of quantitative trait loci (QTL) for agronomic important characters by the use of a RFLP map in barley (Hordeum vulgare L). Theor Appl Genet 90:294–302

    Article  CAS  Google Scholar 

  • Backes G, Madsen LH, Jaiser H, Stougaard J, Herz M, Mohler V, Jahoor A (2003) Localization of genes for resistance against Blumeria graminis f. sp. hordei and Puccinia graminis in a cross between a barley cultivar and a wild barley (Hordeum vulgare ssp. spontaneum) line. Theor Appl Genet 106:353–362

    CAS  PubMed  Google Scholar 

  • Becker J, Heun M (1995) Barley microsatellites: allele variation and mapping. Plant Mol Biol 27:835–845

    CAS  PubMed  Google Scholar 

  • Borovkova IG, Jin Y, Steffenson BJ (1998) Chromosomal location and genetic relationship of leaf rust resistance genes Rph9 and Rph12 in barley. Phytopathology 88:76–80

    Google Scholar 

  • Christensen AB, Gregersen PL, Schröder J, Collinge DB (1998) A chalcone synthase with an unusual substrate preference is expressed in barley leaves in response to UV light and pathogen attack. Plant Mol Biol 37:849–857

    Article  CAS  PubMed  Google Scholar 

  • Costa JM, Corey A, Hayes PM, Jobet C, Kleinhofs A, Kopisch-Obusch A, Kramer SF, Kudrna D, Li M, Riera-Lizarazu O, Sato K, Szucs P, Toojinda T, Vales MJ, Wolfe RI (2001) Molecular mapping of the Oregon Wolfe Barleys: a phenotypically polymorphic doubled-haploid population. Theor Appl Genet 103:415–424

    Article  CAS  Google Scholar 

  • Falak I, Falk DE, Tinker NA, Mather DE (1999) Resistance to powdery mildew in a doubled haploid barley population and association with marker loci. Euphytica 107:185–192

    Article  CAS  Google Scholar 

  • Faris JD, Li WL, Liu DJ, Chen PD, Gill BS (1999) Candidate gene analysis of quantitative disease resistance in wheat. Theor Appl Genet 98:219–225

    Article  CAS  Google Scholar 

  • Feuerstein U, Brown AHD, Burdon JJ (1990) Linkage of rust resistance genes from wild barley (Hordeum spontaneum) with isozyme markers. Plant Breed 104:318–324

    CAS  Google Scholar 

  • Fischbeck G, Jahoor A (1991) The transfer of genes for mildew resistance from Hordeum spontaneum. In: Jorgensen JH (ed) Integrated control of cereal mildews: virulence patterns and their change. Riso National Laboratory, Denmark, pp 247–255

    Google Scholar 

  • Franckowiak JD, Jin Y, Steffenson BJ (1997) Recommended allele symbols for leaf rust resistance genes in barley. Barley Genet Newsl 27:36–44

    Google Scholar 

  • Garvin DF, Brown AHD, Raman H, Read BJ (2000) Genetic mapping of the barley Rrs14 scald resistance gene with RFLP, isozyme and seed storage protein markers. Plant Breed 119:193–196

    Article  CAS  Google Scholar 

  • Genger RK, Williams KJ, Raman H, Wallwork H, Burdon JJ, Brown AHD (2003) Leaf scald resistance genes in Hordeum vulgare and Hordeum vulgare ssp. spontaneum: parallels between cultivated and wild barley. Aust J Agric Res 54:1335–1342

    Article  CAS  Google Scholar 

  • Giese H, Holm-Jensen AG, Jensen HP, Jensen J (1993) Localization of the Laevigatum powdery mildew resistance gene to barley chromosome 2 by the use of RFLP markers. Theor Appl Genet 85:897–900

    Google Scholar 

  • Graner A, Tekauz A (1996) RFLP mapping in barley of a dominant gene conferring resistance to scald (Rhynchosporium secalis). Theor Appl Genet 93:704–771

    Google Scholar 

  • Ivandic V, Walther U, Graner A (1998) Molecular mapping of a new gene in wild barley conferring complete resistance to leaf rust (Puccinia hordei Otth.). Theor Appl Genet 97:1235–1239

    Google Scholar 

  • Jensen J, Backes G, Skinnes H, Giese H (2002) Quantitative trait loci for scald resistance in barley localized by a non-interval mapping procedure. Plant Breed 121:124–128

    Article  CAS  Google Scholar 

  • Jin Y, Cui GH, Steffenson BJ, Franckowiak JD (1996) New leaf rust resistance genes in barley and their allelic relationship with other Rph genes. Phytopathology 86:887–890

    Google Scholar 

  • Jorgensen HJ (1994) Genetics of powdery mildew resistance in barley. Crit Rev Plant Sci 13:97–119

    Google Scholar 

  • Karakousis A, Gustafson JP, Chalmers KJ, Barr AR, Langridge P (2003) A consensus map of barley integrating SSR, RFLP, and AFLP markers. Aust J Agric Res 54:1173–1185

    Article  CAS  Google Scholar 

  • Kicherer S, Backes G, Walther U, Jahoor A (2000) Localising QTLs for leaf rust resistance and agronomic traits in barley (Hordeum vulgare L.). Theor Appl Genet 100:881–888

    Google Scholar 

  • Kleinhofs A, Graner A (2001) An integrated map of the barley genome. In: Phillips RL, Vasil IK (eds) DNA-based markers in plants. Kluwer, Dordrecht, pp 187–199

    Google Scholar 

  • von Korff M, Plümpe J, Michalek W, Léon J, Pillen K (2004a) Insertion of 18 new SSR markers in the Oregon Wolfe Barley map. Barley Genet Newsl 34:1–4

    Google Scholar 

  • von Korff M, Wang H, Léon J, Pillen K (2004b) Development of candidate introgression lines using an exotic barley accession (H. vulgare ssp. spontaneum) as donor. Theor Appl Genet 109:1736–1745

    Google Scholar 

  • Kürth J, Kolsch R, Simons V, Schulze-Lefert P (2001) A high-resolution genetic map and a diagnostic RFLP marker for the Mlg resistance locus to powdery mildew in barley. Theor Appl Genet 102:53–60

    Google Scholar 

  • Laurie DA, Pratchett N, Bezant JH, Snape JW (1995) RFLP mapping of five major genes and eight quantitative trait loci controlling flowering time in a winter × spring barley (Hordeum vulgare L.) cross. Genome 38:575–585

    CAS  Google Scholar 

  • Leah R, Tommerup H, Svensen I, Mundy J (1991) Biochemical and molecular characterisation of three barley seed proteins with antifungal properties. J Biol Chem 266:1564–1573

    CAS  PubMed  Google Scholar 

  • Li ZK, Luo LJ, Mei HW, Paterson AH, Zhao XZ, Zhong DB, Wang YP, Yu XQ, Zhu L, Tabien R, Stansel JW, Ying CS (1999) A ‘defeated’ rice resistance gene acts as a QTL against a virulent strain of Xanthomonas oryzae pv. oryzae. Mol Gen Genet 261:58–63

    Article  CAS  PubMed  Google Scholar 

  • Li JZ, Sjakste TG, Röder MS, Ganal MW (2003) Development and genetic mapping of 127 new microsatellite markers in barley. Theor Appl Genet 107:1021–1027

    Google Scholar 

  • Liu Z-W, Biyashev RM, Saghai Maroof MA (1996) Development of simple sequence repeat DNA markers and their integration into a barley linkage map. Theor Appl Genet 93:869–876

    CAS  Google Scholar 

  • Madsen LH, Collins NC, Rakwalska M, Backes G, Sandal N, Krusell L, Jensen J, Waterman EH, Jahoor A, Ayliffe M, Pryor AJ, Langridge P, Schulze-Lefert P, Stougaard J (2003) Barley disease resistance gene analogs of the NBS–LRR class: identification and mapping. Mol Genet Genomics 269:150–161

    CAS  PubMed  Google Scholar 

  • McDonald BC, Linde C (2002) The population genetics of plant pathogens and breeding strategies for durable resistance. Euphytica 124:163–180

    Article  CAS  Google Scholar 

  • Park RF, Karakousis A (2002) Characterization and mapping of gene Rph19 conferring resistance to Puccinia hordei in the cultivar ‘Reka 1’ and several Australian barleys. Plant Breed 121:232–236

    Article  CAS  Google Scholar 

  • Pillen K, Binder A, Kreuzkam B, Ramsay L, Waugh R, Förster J, Léon J (2000) Mapping new EMBL-derived barley microsatellites and their use in differentiating German barley cultivars. Theor Appl Genet 101:652–660

    CAS  Google Scholar 

  • Pillen K, Zacharias A, Léon J (2003) Advanced backcross QTL analysis in barley (Hordeum vulgare L). Theor Appl Genet 107:340–352

    Google Scholar 

  • Pillen K, Zacharias A, Léon J (2004) Comparative AB-QTL analysis in barley using a single exotic donor of Hordeum vulgare ssp. spontaneum. Theor Appl Genet 108:1591–1601

    Google Scholar 

  • Qi X, Niks RE, Stam P, Lindhout P (1998) Identification of QTLs for partial resistance to leaf rust (Puccinia hordei) in barley. Theor Appl Genet 96:1205–1215

    Google Scholar 

  • Qi X, Fufa F, Sijtsma D, Niks RE, Lindhout P, Stam P (2000) The evidence for abundance of QTLs for partial resistance to Puccinia hordei on the barley genome. Mol Breed 6:1–9

    Article  CAS  Google Scholar 

  • Ramsay L, Macaulay M, degli Ivanissevich S, Maclean K, Cardle L, Fuller J, Edwards KJ, Tuvesson S, Morgante M, Massari A, Maestri E, Marmiroli N, Sjakste T, Ganal M, Powell W, Waugh R (2000) A simple sequence repeat-based linkage map of barley. Genetics 156:1997–2005

    CAS  PubMed  Google Scholar 

  • Read BJ, Raman H, McMichael G, Chalmers KJ, Ablett GA, Platz GJ, Raman R, Genger RK, Boyd WJR, Li CD, Grime CR, Park RF, Wallwork H, Pragnell R, Lance RCM (2003) Mapping and QTL analysis of the barley population Sloop × Halycon. Aust J Agric Res 54:1145–1153

    Article  CAS  Google Scholar 

  • SAS Institute (1999) The SAS system for Windows, release 8.00. Cary

  • Sayed H, Backes G, Kayyal H, Yahyaoui A, Ceccarelli S, Grando S, Jahoor A, Baum M (2004) New molecular markers linked to qualitative and quantitative powdery mildew and scald resistance genes in barley for dry areas. Euphytica 135:225–228

    Article  CAS  Google Scholar 

  • Schönfeld M, Ragni A, Fischbeck G, Jahoor A (1996) RFLP mapping of three new loci for resistance genes to powdery mildew (Erysiphe graminis f. sp. hordei) in barley. Theor Appl Genet 93:48–56

    Google Scholar 

  • Schweizer GF, Baumer M, Daniel G, Rugel H, Röder MS (1995) RFLP markers linked to scald (Rhynchosporium secalis) resistance gene Rh2 in barley. Theor Appl Genet 90:920–924

    Google Scholar 

  • Spaner D, Shugar LP, Choo TM, Falak E, Briggs KG, Legge WG, Falk DE, Ullrich SE, Tinker NA, Steffenson BJ, Mather DE (1998) Mapping of disease resistance loci in barley on the basis of visual assessment of naturally occurring symptoms. Crop Sci 38:843–850

    Google Scholar 

  • Tanksley SD, Nelson JC (1996) Advanced backcross QTL analysis: a method of the simultaneous discovery and transfer of valuable QTLs from unadapted germplasm into elite breeding lines. Theor Appl Genet 92:191–203

    Google Scholar 

  • Thiel T, Michalek W, Varshney RK, Graner A (2003) Exploiting EST databases for the development and characterization of gene-derived SSR-markers in barley (Hordeum vulgare L). Theor Appl Genet 106:411–422

    CAS  PubMed  Google Scholar 

  • Thomas WTB, Baird E, Fuller JD, Lawrence P, Young GR, Russell J, Ramsay L, Waugh R, Powell W (1998) Identification of a QTL decreasing yield in barley linked to Mlo powdery mildew resistance. Mol Breed 4:381–393

    Article  CAS  Google Scholar 

  • Williams KJ (2003) The molecular genetics of disease resistance in barley. Aust J Agric Res 54:1065–1079

    CAS  Google Scholar 

  • Williams KJ, Platz GJ, Barr AR, Cheong J, Willsmore K, Cakir M, Wallwork H (2003) A comparison of the genetics of seedling and adult plant resistance to the spot form of net blotch (Pyrenophora teres f. maculata). Aust J Agric Res 54:1387–1394

    Article  CAS  Google Scholar 

  • Wu J-L, Sinha PK, Variar M, Zheng K-L, Leach JE, Courtois B, Leung H (2004) Association between molecular markers and blast resistance in an advanced backcross population of rice. Theor App Genet 108:1024–1032

    Article  CAS  Google Scholar 

  • Zeller FJ (1998) Nutzung des genetischen Potentials der Hordeum-Wildarten zur Verbesserung der Kulturgerste (Hordeum vulgare L.). J Appl Bot 72:162–167

    Google Scholar 

  • Zhou FS, Kurth JC, Wei FS EC, Vale G, Yahaiaoui N, Keller B, Somerville S, Wise R, Schulze-Lefert P (2001) Cell-autonomous expression of barley Mla1 confers race-specific resistance to the powdery mildew fungus via Rar1 independent signalling pathway. Plant Cell 13:337–350

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgements

We thank E. Laubach (Nordsaat Saatzucht), J. Breun (Breun Saatzucht Josef Breun) and V. Lein (Saaten Union Recherche, Estrées) and their teams for conducting the field experiments. The excellent technical assistance of M. Noschinski, C. Golletz, and the team of our experimental station Dikopshof in Wesseling is appreciated. This work was funded by the German Plant Genome Research Initiative (GABI) of the Federal Ministry of Education and Research (BMBF, project 0312278A).

Author information

Authors and Affiliations

  1. Department of Crop Science & Plant Breeding, University of Bonn, Katzenburgweg 5, 53115, Bonn, Germany

    M. von Korff, H. Wang, J. Léon & K. Pillen

Authors
  1. M. von Korff
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. H. Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. J. Léon
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. K. Pillen
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to K. Pillen.

Additional information

Communicated by G. Wenzel

Rights and permissions

Reprints and permissions

About this article

Cite this article

von Korff, M., Wang, H., Léon, J. et al. AB-QTL analysis in spring barley. I. Detection of resistance genes against powdery mildew, leaf rust and scald introgressed from wild barley. Theor Appl Genet 111, 583–590 (2005). https://doi.org/10.1007/s00122-005-2049-x

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00122-005-2049-x

Keywords

Search

Navigation