Abstract
Opportunities exist for replacing reduced height (Rht) genes Rht-B1b and Rht-D1b with alternative dwarfing genes for bread wheat improvement. In this study, the chromosomal locations of several height-reducing genes were determined by screening populations of recombinant inbred lines or doubled haploid lines varying for plant height with microsatellite markers. Linked markers were found for Rht5 (on chromosome 3BS), Rht12 (5AL) and Rht13 (7BS), which accounted for most of the phenotypic variance in height in the respective populations. Large height differences between genotypes (up to 43 cm) indicated linkage to major height-reducing genes. Rht4 was associated with molecular markers on chromosome 2BL, accounting for up to 30% of the variance in height. Confirming previous studies, Rht8 was linked to markers on chromosome 2DS, whereas a population varying for Rht9 revealed a region with a small but significant height effect on chromosome 5AL. The height-reducing effect of these dwarfing genes was repeatable across a range of environments. The molecular markers developed in this study will be useful for marker-assisted selection of alternative height-reducing genes, and to better understand the effects of different Rht genes on wheat growth and agronomic performance.
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References
Allan RE, Vogel OA, Peterson CJ (1962) Seedling emergence rate of fall-sown wheat and its association with plant height and coleoptile length. Agron J 54:347–350
Bonnett DG, Ellis MH, Rebetzke GJ, Condon AG, Spielmeyer W, Richards RA (2001) Dwarfing genes in Australian wheat—present and future. In: Proceedings of the 10th Australian wheat breeders assembly (Mildura, 16–21 September 2001) pp 154–157
Ellis MH, Rebetzke GJ, Chandler P, Bonnett D, Spielmeyer W, Richards RA (2004) The effect of different height reducing genes on early growth characteristics of wheat. Funct Plant Biol 31(6):583–589
Evans LT (1993) Crop evolution, adaptation, and yield. Cambridge University Press, Cambridge
Gale MD, Law CN, Marshall GA, Snape JW, Worland AJ (1982) Analysis and evaluation of semi-dwarfing genes including a major height reducing gene in the variety ‘Sava’. In: Semi-dwarf cereal mutants and their use in cross-breeding, research coordination meeting, 2–6 March 1981. International Atomic Energy Agency, Vienna, Austria, pp 7–23
Gale MD, Youssefian S, Russell GE (1985) Dwarfing genes in wheat. In: Progress in plant breeding. Butterworths, London, pp 1–35
Konzak CF (1988) Genetic analysis, genetic improvement and evaluation of induced semi-dwarf mutants in wheat. Semi-dwarf cereal mutants and their use in cross-breeding III research coordination meeting, 16–20 December 1985. International Atomic Energy Agency, Vienna, Austria, pp 39–50
Korzun V, Röder MS, Worland AJ, Börner A (1997) Intrachromosomal mapping of genes for dwarfing (Rht12) and vernalization response (Vrn1) in wheat by using RFLP and microsatellite markers. Plant Breed 116:227–232
Korzun V, Röder MS, Ganal MW, Worland AJ, Law CN (1998) Genetic analysis of the dwarfing gene (Rht8) in wheat Part I. Molecular mapping of Rht8 to the short arm of chromosome 2D of bread wheat (Triticum aestivum L). Theor Appl Genet 96:1104–1109
Lincoln SE, Daly MJ, Lander EJ (1993) MAPMAKER/EXP Version 3 and MAPMAKER/QTL Version 1.1. Whitehead Institute, Cambridge
Lorenzetti R (2000) Wheat science. The green revolution of Nazareno Strampelli. J Genet Breed (special publication) p 40
Peng J, Richards DE, Hartley NM, Murphy GP, Devos KM, Flintham JE, Beales J, Fish LJ, Worland AJ, Pelica F, Duralalagaraja S, Christou P, Snape JW, Gale MD, Harberd NP (1999) ‘Green revolution’ genes encode mutant gibberellin response modulators. Nature 400:256–261
Rebetzke GJ, Richards RA, Fischer VM, Mickelson BJ (1999) Breeding long coleoptile, reduced height wheats. Euphytica 106:159–168
Rebetzke GJ, Richards RA (2000) Giberellic acid-sensitive wheats reduce plant height to increase kernel number and grain yield of wheat. Aust J Agric Res 51:251–245
Rebetzke GJ, Appels R, Morrison AD, Richards, RA, McDonald G, Ellis MH, Spielmeyer W, Bonnett DG (2001) Quantitative trait loci on chromosome 4B for coleoptile length and early vigour in wheat (Triticum aestivum L). Aust J Agric Res 52:1221–1234
Richards RA (1992) The effect of dwarfing genes in spring wheat in dry environments I Agronomic characteristics. Aust J Agric Res 43:517–527
Richards RA, Lukacs Z (2002) Seedling vigour in wheat—sources of variation for genetic and agronomic improvement. Aust J Agric Res 53:41–50
Röder MS, Korzun V, Wendehake K, Plaschke J, Tixier MH, Leroy P, Ganal MW (1998) A microsatellite map of wheat. Genet 149:2007–2023
SAS Institute Inc (1990) SAS/STATO User’s guide, Version 6, 4th edn, vol 1. SAS Institute Inc, Cary
Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor
Singh RP, Huerta-Espino J, Rajaram S, Crossa J (2001) Grain yield and other traits of tall and dwarf isolines of modern bread and durum wheats. Euphytica 119:241–244
Spielmeyer W, Bonnett DG, Ellis MH, Rebetzke GJ, Richards RA (2001) Implementation of molecular markers to improve selection efficiency in CSIRO wheat breeding program. In: Proceedings of the 10th Australian wheat breeders assembly, Mildura, 16–21 September 2001, pp 88–91
Spielmeyer W, Ellis MH, Robertson M, Ali S, Lenton JR, Chandler PM (2004) Isolation of gibberellin and metabolic pathway genes from barley and comparative mapping in barley, wheat and rice. Theor Appl Genet 109(4):847–855
Stapper M, Fischer RA (1990) Genotype, sowing date and plant spacing influence on high-yielding irrigated wheat in southern New South Wales III Potential yields and optimum flowering dates. Aust J Agric Res 41:1046–1056
Whan BR (1976) The association between coleoptile length and culm length in semi-dwarf and standard wheats. J Aust Inst Agric Sci 42:194–196
Acknowledgements
This work is supported by Graingene—a research joint venture between the Australian Wheat Board limited, CSIRO, the Grains Research and Development Corporation and Syngenta seeds. We thank Arnaud Serin, Paul Joaquim, Bernie Mickleson and Kylie Groom for technical assistance.
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Communicated by J. W. Snape
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Ellis, M.H., Rebetzke, G.J., Azanza, F. et al. Molecular mapping of gibberellin-responsive dwarfing genes in bread wheat. Theor Appl Genet 111, 423–430 (2005). https://doi.org/10.1007/s00122-005-2008-6
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DOI: https://doi.org/10.1007/s00122-005-2008-6