Abstract
Tuber eye depth of the potato (Solanum tuberosum L.) is an important trait for the processing quality and appearance of potatoes. In the present study, we used a cultivated diploid potato family (12601) of 107 plants to dissect the mode of inheritance and to map the gene(s) controlling the trait. The family segregated for both eye depth (deep vs shallow) and tuber shape (round vs long) traits. The deep eye (Eyd) phenotype was found to be associated with round tubers (Ro) in most progeny clones. Further evaluation of this population with molecular markers including simple sequence repeats, amplified fragment length polymorphism, and sequence-characterized amplified regions revealed that the primary locus for eye depth is located on chromosome 10. This map location was confirmed by evaluating a second diploid family (12586). The results of this study led to the following conclusions: (1) there is a major locus controlling the eye depth trait; (2) deep eye (Eyd) is dominant to shallow (eyd); (3) the Eyd/eyd locus is located on chromosome 10; and (4) the Eyd/eyd locus is closely linked with the major locus for tuber shape (Ro/ro), at a distance of about 4 cM.
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Acknowledgements
The authors thank Drs. S. Tanksley, Cornell University, and C. Gebhardt, Max Planck Institute, for kindly providing anchor marker clones for chromosome 10. We thank Dr. Richard Tarn, Agnes Murphy, Valerie Burns and Ken Paddock as well as the greenhouse staff at the Potato Research Centre and the staff of the Benton Ridge Potato Breeding Substation for maintaining the potato clones. The scientific discussions with Dr. George Tai are much appreciated. We also thank Jiazheng (John) Yuan, Muhammad Haroon, Birt Stevens, Tieling Zhang, and Dr. Kerry Ready for technical assistance in nucleotide sequencing and in testing the segregation of molecular markers in the population 12601.
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Li, XQ., De Jong, H., De Jong, D.M. et al. Inheritance and genetic mapping of tuber eye depth in cultivated diploid potatoes. Theor Appl Genet 110, 1068–1073 (2005). https://doi.org/10.1007/s00122-005-1927-6
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DOI: https://doi.org/10.1007/s00122-005-1927-6