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Construction of an oilseed rape (Brassica napus L.) genetic map with SSR markers

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Abstract

We constructed a Brassica napus genetic map with 240 simple sequence repeats (SSR) primer pairs from private and public origins. SSR, or microsatellites, are highly polymorphic and efficient markers for the analysis of plant genomes. Our selection of primer pairs corresponded to 305 genetic loci that we were able to map. In addition, we also used 52 sequence-characterized amplified region primer pairs corresponding to 58 loci that were developed in our lab. Genotyping was performed on six F2 populations, corresponding to a total of 574 F2 individual plants, obtained according to an unbalanced diallel cross design involving six parental lines. The resulting consensus map presented 19 linkage groups ranging from 46.2 to 276.5 cM, which we were able to name after the B. napus map available at http://ukcrop.net/perl/ace/search/BrassicaDB, thus enabling the identification of the A genome linkage groups originating from the B. rapa ancestor and the C genome linkage groups originating from the B. oleracea ancestor in the amphidiploid genome of B. napus. Some homoeologous regions were identified between the A and the C genomes. This map could be used to identify more markers, which would eventually be linked to genes controlling important agronomic characters in rapeseed. Furthermore, considering the good genome coverage we obtained, together with an observed homogenous distribution of the loci across the genome, this map is a powerful tool to be used in marker-assisted breeding.

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References

  • Budin K, Just J, Thareau V, Belcram H, Moreau S, Laumonier M, Lecharny A, Aubourg S, Caboche M, Chalhoub B (2004) Physical mapping using new comparative genomic-based methods: functional physical markers for BAC-based happy mapping and contiguing of BAC clones in Brassica napus. Plant & Animal Genomes XIIth Conference, January 10–14, San Diego, CA

  • Cheung WY, Champagne G, Hubert N, Landry BS (1997) Comparison of the genetic maps of Brassica napus and Brassica oleracea. Theor Appl Genet 94:569–582

    Article  CAS  Google Scholar 

  • Dellaporta SL, Wood J, Hicks JB (1983) A plant DNA minipreparation: version II. Plant Mol Biol Reporter 1(4):19–21

    CAS  Google Scholar 

  • Ferreira ME, Satagopan J, Yandell BS, Williams PH, Osborn TC (1994) RFLP mapping of Brassica napus using doubled-haploid lines. Theor Appl Genet 89:615–621

    Article  CAS  Google Scholar 

  • Foisset N, Delourme R (1996) Segregation distorsion in androgenic plants. In: Mohan Jain S, Sopory S, Veilleux R (eds) In vitro haploid production in higher plants. Kluwer, Dordrecht, pp 189–201

    Google Scholar 

  • Foisset N, Delourme R, Barret P, Hubert N, Landry BS, Renard M (1996) Molecular mapping analysis in Brassica napus using isozyme, RAPD and RFLP markers on a doubled-haploid progeny. Theor Appl Genet 93:1017–1025

    Article  CAS  Google Scholar 

  • Landry BS, Hubert N, Etoh T, Harada J, Lincoln SE (1991) A genetic map for Brassica napus based on restriction fragment length polymorphisms detected with expressed DNA sequences. Genome 34:543–552

    CAS  Google Scholar 

  • Lombard V, Delourme R (2001) A consensus linkage map for rapeseed (Brassica napus L): construction and integration of three individual maps from DH populations. Theor Appl Genet 103:491–507

    Article  CAS  Google Scholar 

  • Lowe AJ, Moule C, Trick M, Edwards KJ (2004) Efficient large-scale development of microsatellites for marker and mapping applications in Brassica crop species. Theor Appl Genet 108:1103–1112

    Article  PubMed  CAS  Google Scholar 

  • Osborn TC, Kole C, Parkin IA, Sharpe AG, Kuiper M, Lydiate DJ, Trick M (1997) Comparison of flowering time genes in Brassica rapa, B. napus and Arabidopsis thaliana. Genetics 146:1123–1129

    PubMed  CAS  Google Scholar 

  • Parkin IAP, Lydiate D (1997) Conserved patterns of chromosome pairing and recombination in Brassica napus crosses. Genome 40:496–504

    Article  CAS  PubMed  Google Scholar 

  • Parkin IAP, Sharpe AG, Keith DJ, Lydiate DJ (1995) Identification of the A and C genomes of amphidiploid Brassica napus (oilseed rape). Genome 38:1122–1131

    CAS  PubMed  Google Scholar 

  • Parkin IAP, Sharpe AG, Lydiate DJ (2003) Patterns of genome duplication within the Brassica napus genome. Genome 46:291–303

    Article  PubMed  CAS  Google Scholar 

  • Plieske J, Struss D (2001) Microsatellite markers for genome analysis in Brassica. I. Development in Brassica napus and abundance in Brassicaceae species. Theor Appl Genet 102:689–694

    Article  CAS  Google Scholar 

  • Saal B, Plieske J, Hu J, Quiros CF and Struss D (2001) Microsatellite markers for genome analysis in Brassica. II. Assignment of rapeseed microsatellites to the A and C genomes and genetic mapping in Brassica oleracea L. Theor Appl Genet 102:695–699

    Article  CAS  Google Scholar 

  • Schiex T, Gaspin C (1997) CARTHAGENE: constructing and joining maximum-likelihood genetic maps. In: Proceedings of the fifth international conference of intelligent systems for molecular biology, Porto Caras, Halkidiki, Greece, pp 258–267

  • Sharpe AG, Parkin IAP, Keith DJ, Lydiate DJ (1995) Frequent non reciprocal translocations in the amphidiploid genome of oilseed rape (Brassica napus). Genome 38:1112–1121

    CAS  PubMed  Google Scholar 

  • U N (1935) Genome analysis in Brassica with special reference to the experimental formation of B. napus and peculiar mode of fertilization. Jpn J Bot 7:389–452

    Google Scholar 

  • Udall JA, Quijada PA, Osborn TC (2005) Detection of chromosomal rearrangements derived from homoeologous recombination in four mapping populations of Brassica napus L. Genetics 169:967–979

    Article  PubMed  CAS  Google Scholar 

  • Uzunova MI, Ecke W (1999) Abundance, polymorphism and genetic mapping of microsatellites in oilseed rape (Brassica napus L). Plant Breed 118:323–326

    Article  CAS  Google Scholar 

  • Uzunova M, Ecke W, Weissleder K, Robbelen G (1995) Mapping the genome of rapeseed (Brassica napus L.) I. Construction of an RFLP linkage map and localization of QTLs for seed glucosinolate content. Theor Appl Genet 90:194–204

    Article  CAS  Google Scholar 

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Acknowledgements

The authors are grateful to Regine Delourme, from the UMR INRA ENSAR at Le Rheu, for giving us HD lines from the Darmor X Yudal population. We also thank Marika Seletti who gracefully accepted to review the English version of the manuscript.

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Authors and Affiliations

  1. Euralis Semences laboratoire de génétique moléculaire, Domaine de Sandreau, 31700, Mondonville, France

    J. Piquemal, E. Cinquin, F. Couton, C. Rondeau, E. Seignoret, I. doucet, D. Perret, M.-J. Villeger, P. Vincourt & P. Blanchard

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  1. J. Piquemal
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  2. E. Cinquin
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  3. F. Couton
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  4. C. Rondeau
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  5. E. Seignoret
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  6. I. doucet
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  7. D. Perret
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  8. M.-J. Villeger
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  9. P. Vincourt
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  10. P. Blanchard
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Correspondence to J. Piquemal.

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Communicated by H. C. Becker

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Piquemal, J., Cinquin, E., Couton, F. et al. Construction of an oilseed rape (Brassica napus L.) genetic map with SSR markers. Theor Appl Genet 111, 1514–1523 (2005). https://doi.org/10.1007/s00122-005-0080-6

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  • DOI: https://doi.org/10.1007/s00122-005-0080-6

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