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Genetic distances revealed by morphological characters, isozymes, proteins and RAPD markers and their relationships with hybrid performance in oilseed rape (Brassica napus L.)

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Abstract

Genetic distances (GDs) based on morphological characters, isozymes and storage proteins, and random amplified polymorphic DNAs (RAPD) were used to predict the performance and heterosis of crosses in oilseed rape (Brassica napus L.). Six male-sterile lines carrying the widely used Shaan2A cytoplasm were crossed with five restorer lines to produce 30 F1 hybrids. These 30 hybrids and their parents were evaluated for seven agronomically important traits and their mid-parent heterosis (MPH) at Yangling, Shaanxi province in Northwest China for 2 years. Genetic similarity among the parents based on 34 isozyme and seven protein markers was higher than that based on 136 RAPDs and/or 48 morphological markers. No significant correlation was detected among these three sets of data. Associations between the different estimates of GDs and F1 performance for some agronomic traits were significant, but not for seed yield. In order to enhance the predicting efficiency, we selected 114 significant markers and 43 favoring markers following statistical comparison of the mean values of the yield components between the heterozygous group (where the marker is present only in one parent of each hybrid) and the homozygous group (where the marker is either present or absent in both parents of each hybrid) of the 30 hybrids. Parental GD based on total polymorphic markers (GDtotal, indicating general heterozygosity), significant markers (GDsign, indicating specific heterozygosity) and favoring markers (GDfavor, indicating favoring-marker heterozygosity) were calculated. The correlation between GDfavor or GDsign and hybrid performance was higher than the correlation between GDtotal and hybrid performance. GDsign and GDfavor significantly correlated with plant height, seeds per silique and seed yield, but not with the MPH of the other six agronomic traits with the exception of plant height. The information obtained in this study on the genetic diversity of the parental lines does not appear to be reliable for predicting F1 yield and heterosis.

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Acknowledgements

The authors thank C.H. Zhang and Y.J. Yu, College of Agronomy, Northwest Sci-Tech University of Agriculture and Forestry for their assistance in both the laboratory and the field. This work was supported by Chinese Chunhui Project (Z2004-1-61007) and Sino-Czech Sci-Tech Cooperative Project (37-18).

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Authors and Affiliations

  1. College of Agronomy, Northwest Sci-Tech University of Agriculture and Forestry, Yangling, Shaanxi, 712100, P.R. China

    C. Y. Yu & S. W. Hu

  2. College of Life Sciences, Northwest Sci-Tech University of Agriculture and Forestry, Yangling, Shaanxi, 712100, P.R. China

    H. X. Zhao & A. G. Guo

  3. Department of Biology, Saint Mary’s University, Nova Scotia, Halifax, B3H 3C3, Canada

    G. L. Sun

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  1. C. Y. Yu
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  2. S. W. Hu
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Correspondence to S. W. Hu.

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Communicated by H.C. Becker

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Yu, C.Y., Hu, S.W., Zhao, H.X. et al. Genetic distances revealed by morphological characters, isozymes, proteins and RAPD markers and their relationships with hybrid performance in oilseed rape (Brassica napus L.). Theor Appl Genet 110, 511–518 (2005). https://doi.org/10.1007/s00122-004-1858-7

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