Skip to main content
SpringerLink
Log in

Transfer of tuber soft rot and early blight resistances from Solanum brevidens into cultivated potato

  • Original Paper
  • Published:
Theoretical and Applied Genetics Aims and scope Submit manuscript

Abstract

Tuber soft rot and early blight are serious potato diseases. Development of potato varieties resistant to these diseases has been hindered by the scarcity of resistant germplasm. A diploid wild species, Solanum brevidens, shows significant resistance to both diseases. Numerous potato breeding lines have been developed from a potato-S. brevidens somatic hybrid, A206. A BC3 clone, C75-5+297, derived from this somatic hybrid as well as its BC1 and BC2 parental lines showed resistance to both tuber soft rot and early blight. Clone C75-5+297 has consistently out-yielded common varieties under disease stress. Using both molecular and cytogenetic approaches we demonstrated that a single copy of chromosome 8 from S. brevidens replaced a potato chromosome 8 in C75-5+297. Thus, C75-5+297 represents a potato-S. brevidens chromosome substitution line. Our results suggest that the presence of a single chromosome from S. brevidens may significantly impact the resistance to multiple potato diseases. The high yield potential of C75-5+297 makes it an excellent parent for developing potato varieties with resistances to both tuber soft rot and early blight.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2A, B
Fig. 3A–D

Similar content being viewed by others

References

  • Austin S, Ehlenfeldt MK, Baer MA, Helgeson JP (1986) Somatic hybrids produced by protoplast fusion between Solanum tuberosum and Solanum brevidens: phenotypic variation under field conditions. Theor Appl Genet 71:682–690

    Google Scholar 

  • Austin S, Lojkowska E, Ehlenfeldt MK, Kelman A, Helgeson JP (1988) Fertile interspecific somatic hybrids of Solanum: a novel source of resistance to Erwinia soft rot. Phytopathology 78:1216–1220

    Google Scholar 

  • Boiteux LS, Reifschneider FJB, Fonseca MEN, Buso JA (1995) Search for sources of early blight (Alternaria solani) field-resistance not associated with vegetative late maturity in tetraploid potato germplasm. Euphytica 83:63–70

    Google Scholar 

  • Bradeen JM, Naess SK, Song J, Haberlach GT, Wielgus SM, Buell CR, Jiang J, Helgeson JP (2003) Concomitant reiterative BAC walking and fine genetic mapping enable physical map development for the broad-spectrum late blight resistance region, RB. Mol Genet Genomics 269:603–611

    Article  CAS  PubMed  Google Scholar 

  • Dong F, Song J, Naess SK, Helgeson JP, Gebhardt G, Jiang J (2000) Development and applications of a set of chromosome-specific cytogenetic DNA markers in potato. Theor Appl Genet 101:1001–1007

    CAS  Google Scholar 

  • Dong F, McGrath JM, Helgeson JP, Jiang J (2001) The genetic identity of alien chromosomes in potato breeding lines revealed by sequential GISH and FISH analyses using chromosome-specific cytogenetic DNA markers. Genome 44:729–734

    Article  CAS  PubMed  Google Scholar 

  • Gebhardt C, Ritter E, Salamini F (2001) RFLP map of the potato. In: Phillips RL, Vasil IK (eds) DNA-based markers in plants, 2nd edn. Kluwer, Dordrecht, pp 319–336

  • Helgeson JP, Hunt GJ, Haberlach GT, Austin S (1985) Somatic hybrids between Solanum brevidens and Solanum tuberosum: expression of a late blight resistance gene and potato leaf roll resistance. Plant Cell Rep 3:212–214

    Google Scholar 

  • Helgeson JP, Haberlach GT, Ehlenfeldt MK, Hunt G, Pohlman JD, Austin S (1993) Sexual progeny of somatic hybrids between potato and Solanum brevidens: potential for use in breeding programs. Am Potato J 70:437–452

    Google Scholar 

  • Helgeson JP, Pohlman JD, Austin S, Haberlach GT, Wielgus SM, Ronis D, Zambolim L, Tooley P, McGrath JM, James RV, Stevenson WR (1998) Somatic hybrids between Solanum bulbocastanum and potato: a new source of resistance to late blight. Theor Appl Genet 96:738–742

    Google Scholar 

  • Jansky S (2000) Breeding for disease resistance in potato. In: Janick J (ed) Plant breeding reviews. Wiley, New York, pp 69–155

  • McGrath JM, Wielgus SM, Uchytil TF, Kim-Lee H, Haberlach GT, Williams CE, Helgeson JP (1994) Recombination of Solanum brevidens chromosomes in the second backcross generation from a somatic hybrid with S. tuberosum. Theor Appl Genet 88:917–924

    Google Scholar 

  • McGrath JM, Wielgus SM, Helgeson JP (1996) Segregation and recombination of Solanum brevidens synteny groups in progeny of somatic hybrids with S. tuberosum: Intragenomic equals or exceeds intergenomic recombination. Genetics 142:1335–1348

    CAS  PubMed  Google Scholar 

  • McGrath JM, Williams CE, Haberlach GT, Wielgus SM, Uchytil TF, Helgeson JP (2002) Introgression and stabilization of Erwinia tuber soft rot resistance into potato after somatic hybridization of Solanum tuberosum and S. brevidens. Am J Potato Res 79:19–24

    Google Scholar 

  • Naess SK, Bradeen JM, Wielgus SM, Haberlach GT, McGrath JM, Helgeson JP (2000) Resistance to late blight in Solanum bulbocastanum is mapped to chromosome 8. Theor Appl Genet 101:697–704

    Article  CAS  Google Scholar 

  • Naess SK, Bradeen JM, Wielgus SM, Haberlach GT, McGrath JM, Helgeson JP (2001) Analysis of introgression of Solanum bulbocastanum DNA into potato breeding lines. Mol Gen Genomics 265:694–704

    Article  CAS  Google Scholar 

  • Pavek JJ, Corsini DL (2001) Utilization of potato genetic resources in variety development. Am J Potato Res 78:433–441

    Google Scholar 

  • Song J, Bradeen JM, Naess SK, Raasch JA, Wielgus SW, Haberlach GT, Liu J, Kuang H, Austin-Phillips S, Buell CR, Helgeson JP, Jiang J (2003) Gene RB cloned from Solanum bulbocastanum confers broad spectrum resistance to potato late blight. Proc Natl Acad Sci USA 100:9128–9133

    Article  CAS  PubMed  Google Scholar 

  • Stevenson WR (1994) The potential impact of field-resistance to early blight on fungicide inputs. Am Potato J 71:317–324

    Google Scholar 

  • Umaerus V, Umaerus M (1994) Inheritance of resistance to late blight. In: Bradshaw JE, Mackay GR (eds) Potato genetics. CAB International, Wallingford, pp 365–401

  • Williams CE, Hunt GJ, Helgeson JP (1990) Fertile somatic hybrids of Solanum spp.: RFLP analysis of a hybrid and its sexual progeny from crosses with potato. Theor Appl Genet 80:545–551

    Google Scholar 

  • Zimnoch-Guzowska E, Marczewski W, Lebecka R, Flis B, Schafer-Pregl R, Salamini F, Gebhardt C (2000) QTL analysis of new sources of resistance to Erwinia carotovora ssp. atroseptica in potato done by AFLP, RFLP, and resistance-gene-like markers. Crop Sci 40:1156–1167

    CAS  Google Scholar 

Download references

Acknowledgements

A.L.T. acknowledges a scholarship from the Ministry of Turkish National Education for graduate studies. We are grateful to Dr. J.M. McGrath for valuable discussions on the resistance to soft rot, Dr. R.V. James for evaluation of early blight development in the field and Dr. S.K. Naess and S. Wielgus for assistance with the Southern blot hybridizations. This research is partially supported by Hatch funds and funds from Wisconsin Potato & Vegetable Growers Association to J.J.

Author information

Authors and Affiliations

  1. Department of Horticulture, University of Wisconsin, Madison, WI 53706, USA

    Ahmet L. Tek & Jiming Jiang

  2. Department of Plant Pathology, University of Wisconsin, Madison, WI 53706, USA

    Walter R. Stevenson

  3. USDA/ARS and Department of Plant Pathology, University of Wisconsin, Madison, WI 53706, USA

    John P. Helgeson

Authors
  1. Ahmet L. Tek
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Walter R. Stevenson
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. John P. Helgeson
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Jiming Jiang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Jiming Jiang.

Additional information

Communicated by J.S. Heslop-Harrison

Rights and permissions

Reprints and permissions

About this article

Cite this article

Tek, A.L., Stevenson, W.R., Helgeson, J.P. et al. Transfer of tuber soft rot and early blight resistances from Solanum brevidens into cultivated potato. Theor Appl Genet 109, 249–254 (2004). https://doi.org/10.1007/s00122-004-1638-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00122-004-1638-4

Keywords

Search

Navigation