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QTL analysis of resistance to Fusarium head blight in Swiss winter wheat (Triticum aestivum L.)

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Abstract

Fusarium head blight (FHB) of wheat is a widespread and destructive disease which occurs in humid and semi-humid areas. FHB epidemics can cause serious yield and quality losses under favorable climatic conditions, but the major concern is the contamination of grains with mycotoxins. Resistance to FHB is quantitatively inherited and greatly influenced by the environment. Its evaluation is costly and time-consuming. The genetic basis of FHB resistance has mainly been studied in spring wheat. The objective of this study was to map quantitative trait loci (QTLs) for resistance to FHB in a population of 240 recombinant inbred lines (RILs) derived from a cross between the two Swiss winter wheat cultivars Arina (resistant) and Forno (susceptible). The RILs were genotyped with microsatellite and RFLP markers. The resulting genetic map comprises 380 loci and spans 3,086 cM. The 240 RILs were evaluated for resistance to FHB in six field trials over 3 years. Composite interval mapping (CIM) analyses carried out on FHB AUDPC (i.e. mean values across six environments) revealed eight QTLs which altogether explained 47% of the phenotypic variance. The three main QTLs were mapped on the long arms of chromosomes 6D (R 2=22%), 5B (R 2=14%) and 4A (R 2=10%). The QTL detected on 5B originated from the susceptible parent Forno. Other QTLs with smaller effects on FHB resistance were detected on chromosomes 2AL, 3AL, 3BL, 3DS and 5AL.

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Acknowledgements

We thank I. Baenziger (FAL-Reckenholz), B. Senger (University of Zürich) and M. Battini (FAL-Reckenholz) for excellent technical assistance. We are grateful for the mapping information on barc microsatellite markers provided by Dr. R. Ward (Michigan State University). We also appreciated the computer packages PLABSTAT and PLABQTL received from Prof. H.F. Utz (University of Hohenheim, Stuttgart, Germany) and we thank him for his helpful advice in QTL mapping. Dr. L. Moreau (INRA Le Moulon, Gif-sur-Yvette, France) is acknowledged for her helpful advice in using PLABQTL and in QTL mapping. Funding for this work was provided by the Indo-Swiss Collaboration in Biotechnology and grant 3100-065114 from the Swiss National Science Foundation. All experiments conducted during this study comply with current Swiss laws.

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Author notes

  1. S. Paillard

    Present address: Unité de Recherche en Génomique Végétale (URGV), UMR INRA 1165, CNRS 8114, Université d’Evry Val d’Essonne, 2 rue Gaston Crémieux, CP 5708, 91057 , Evry Cedex, France

Authors and Affiliations

  1. Swiss Federal Research Station for Agroecology and Agriculture (FAL-Reckenholz), Reckenholzstrasse 191, 8046 , Zurich, Switzerland

    S. Paillard & M. Winzeler

  2. Institute of Plant Biology, University of Zurich, Zollikerstrasse 107, 8008 , Zurich, Switzerland

    T. Schnurbusch & B. Keller

  3. Directorate of Wheat Research, Karnal, 132 001, Haryana, India

    R. Tiwari

  4. Pharmaceutical Institute, University of Basel, Benkenstrasse 254, 4108 , Witterswil, Switzerland

    M. Messmer

  5. Indo-Swiss Collaboration in Biotechnology (ISCB), Institute of Biotechnology, ETH-Hoenggerberg, 8093 , Zurich, Switzerland

    G. Schachermayr

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  1. S. Paillard
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Correspondence to G. Schachermayr.

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Communicated by H.C. Becker

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Paillard, S., Schnurbusch, T., Tiwari, R. et al. QTL analysis of resistance to Fusarium head blight in Swiss winter wheat (Triticum aestivum L.). Theor Appl Genet 109, 323–332 (2004). https://doi.org/10.1007/s00122-004-1628-6

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  • DOI: https://doi.org/10.1007/s00122-004-1628-6

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