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Quantitative trait loci for lodging resistance, plant height and partial resistance to mycosphaerella blight in field pea (Pisum sativum L.)

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Abstract

With the development of genetic maps and the identification of the most-likely positions of quantitative trait loci (QTLs) on these maps, molecular markers for lodging resistance can be identified. Consequently, marker-assisted selection (MAS) has the potential to improve the efficiency of selection for lodging resistance in a breeding program. This study was conducted to identify genetic loci associated with lodging resistance, plant height and reaction to mycosphaerella blight in pea. A population consisting of 88 recombinant inbred lines (RILs) was developed from a cross between Carneval and MP1401. The RILs were evaluated in 11 environments across the provinces of Manitoba, Saskatchewan and Alberta, Canada in 1998, 1999 and 2000. One hundred and ninety two amplified fragment length polymorphism (AFLP) markers, 13 random amplified polymorphic DNA (RAPD) markers and one sequence tagged site (STS) marker were assigned to ten linkage groups (LGs) that covered 1,274 centi Morgans (cM) of the pea genome. Six of these LGs were aligned with the previous pea map. Two QTLs were identified for lodging resistance that collectively explained 58% of the total phenotypic variation in the mean environment. Three QTLs were identified each for plant height and resistance to mycosphaerella blight, which accounted for 65% and 36% of the total phenotypic variation, respectively, in the mean environment. These QTLs were relatively consistent across environments. The AFLP marker that was associated with the major locus for lodging resistance was converted into the sequence-characterized amplified-region (SCAR) marker. The presence or absence of the SCAR marker corresponded well with the lodging reaction of 50 commercial pea varieties.

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Acknowledgements

Financial support from the Western Grains Research Foundation is gratefully acknowledged. We thank Al Sloan, Brent Barlow and Rob Kirkpatrick for their technical expertise.

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Authors and Affiliations

  1. Crop Development Centre, University of Saskatchewan, Saskatoon, SK, S7N 5A8, Canada

    B. Tar'an, T. Warkentin & A. Vandenberg

  2. Cereal Research Centre, Agriculture and Agri-Food Canada, Winnipeg, MB, R3T 2M9, Canada

    D. J. Somers, D. Miranda & S. Woods

  3. Alberta Agriculture, Food and Rural Development, Edmonton, AB, T5B 4K3, Canada

    S. Blade

  4. Lacombe Research Station, Agriculture and Agri-Food Canada, Lacombe, AB, T4L 1W1, Canada

    D. Bing

  5. Eastern Cereal and Oilseed Research Centre, Agriculture and Agri-Food Canada, Ottawa, ON, K1A 0C6, Canada

    A. Xue

  6. Potato Research Centre, Agriculture and Agri-Food Canada, Fredericton, NB, E3B 4Z7, Canada

    D. DeKoeyer

  7. Monsanto Canada Inc., 67 Scurfield Blvd., Winnipeg, MB, R3Y 1G4, Canada

    G. Penner

Authors
  1. B. Tar'an
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  2. T. Warkentin
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  3. D. J. Somers
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  4. D. Miranda
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  5. A. Vandenberg
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  6. S. Blade
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  7. S. Woods
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  8. D. Bing
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  9. A. Xue
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  10. D. DeKoeyer
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  11. G. Penner
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Correspondence to B. Tar'an.

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Communicated by H. F. Linskens

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Tar'an, B., Warkentin, T., Somers, D.J. et al. Quantitative trait loci for lodging resistance, plant height and partial resistance to mycosphaerella blight in field pea (Pisum sativum L.). Theor Appl Genet 107, 1482–1491 (2003). https://doi.org/10.1007/s00122-003-1379-9

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  • DOI: https://doi.org/10.1007/s00122-003-1379-9

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