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Cytoplasmic male sterility in alloplasmic Brassica juncea carrying Diplotaxis catholica cytoplasm: molecular characterization and genetics of fertility restoration

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Abstract

The present study was aimed at characterizing cytoplasmic male sterility (CMS) and identifying the fertility restorer gene for CMS (Diplotaxis catholica) Brassica juncea derived through sexual hybridization. The fertility restorer gene was identified by crossing the CMS line with progeny plants derived from somatic hybrids of B. juncea and D. cathoilca. The CMS line is comparable to the nuclear donor B. juncea in all respects except for flower and silique characteristics. In CMS plants, the flowers have smaller nectaries, and anthers are converted into petals or tubular structures. Gynoecium exhibits a crooked style and trilocular ovary. Seed fertility was reduced in the CMS line. Genetic segregation data indicated that a single, dominant, nuclear gene governs fertility restoration. Restored plants showed a high female fertility and lacked gynoecium abnormalities. In fertility-restored plants, petal development was found to be variable; some flowers had the normal number of four petals, while others had zero to three petals. Interestingly, the trilocular character of the ovary was found to co-segregate with CMS and became bilocular upon male-fertility restoration. Thus, this trait appears to be affected by the interaction of nuclear and mitochondrial (mt) genomes. Restriction fragment length polymorphism analysis indicated that mt-genome of D. catholica is highly divergent from that of B. juncea. However, in Northern analysis, out of eight mt genes studied, an altered transcript pattern was recorded for only atpA. In fertility-restored plants, the atpA transcript became shorter, thereby showing its association with CMS.

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Acknowledgements

We are grateful to Prof. K. Shivanna, University of Delhi, India for providing the seeds of the CMS line. AP was supported by a student grant from the Council of Scientific and Industrial Research, Government of India.

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  1. National Research Centre on Plant Biotechnology, Indian Agricultural Research Institute, New Delhi 110012, India

    A. Pathania, S. R. Bhat, V. Dinesh Kumar,  Ashutosh, P. B. Kirti, S. Prakash & V. L. Chopra

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  1. A. Pathania
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  2. S. R. Bhat
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  3. V. Dinesh Kumar
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  4. Ashutosh
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  5. P. B. Kirti
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  6. S. Prakash
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  7. V. L. Chopra
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Correspondence to S. R. Bhat.

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Communicated by K. Glimelius

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Pathania, A., Bhat, S.R., Dinesh Kumar, V. et al. Cytoplasmic male sterility in alloplasmic Brassica juncea carrying Diplotaxis catholica cytoplasm: molecular characterization and genetics of fertility restoration. Theor Appl Genet 107, 455–461 (2003). https://doi.org/10.1007/s00122-003-1266-4

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