Skip to main content
SpringerLink
Log in

Production of functional rat liver PSP protein in Escherichia coli

  • Published:
Cellular and Molecular Life Sciences CMLS Aims and scope Submit manuscript

Abstract.

An efficient Escherichia coli expression system for the production of a perchloric acid-soluble protein (PSP) has been constructed. Complementary DNA encoding PSP was inserted into an inducible bacterial expression vector pGEX-4T-1. After the plasmid introduced into E. coli was expressed by isopropyl 1-thio-β-D-galactopyranoside (IPTG), the recombinant product was purified by glutathione-Sepharose 4B affinity chromatography. The purified product showed the expected NH2-terminal sequence, but the translation inhibitory activity of this product was 10 times lower compared with that of authentic PSP isolated from rat liver.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

Author information

Authors and Affiliations

  1. Department of Veterinary Physiology, Faculty of Agriculture, Kagoshima University, Kagoshima 890-0065 (Japan), JP

    T. Oka & Y. Kawasaki

  2. Department of Nutrition, School of Medicine, University of Tokushima, Tokushima 770-8503 (Japan), JP

    Y. Nishimoto, H. Kanouchi & Y. Natori

  3. Department of Nutritional Science, Faculty of Health and Welfare Science, Okayama Prefectural University, Okayama 719-1197 (Japan), Fax +81 99 285 8714, e-mail: oka@vet.agri.kagoshima-u.ac.jp, JP

    T. Sasagawa

Authors
  1. T. Oka
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Y. Nishimoto
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. T. Sasagawa
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. H. Kanouchi
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Y. Kawasaki
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Y. Natori
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Received 8 October 1998; received after revision 6 November 1998; accepted 6 November 1998

Rights and permissions

Reprints and permissions

About this article

Cite this article

Oka, T., Nishimoto, Y., Sasagawa, T. et al. Production of functional rat liver PSP protein in Escherichia coli. CMLS, Cell. Mol. Life Sci. 55, 131–134 (1999). https://doi.org/10.1007/s000180050277

Download citation

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s000180050277

Search

Navigation