Abstract
Mechanoreceptors are implicated as functional afferents within mucosa of the airways and the recent discovery of mechanosensitive channels Piezo1 and Piezo2 has proved essential for cells of various mechanically sensitive tissues. However, the role for Piezo1/2 in vocal fold (VF) mucosal epithelia, a cell that withstands excessive biomechanical insult, remains unknown. The purpose of this study was to test the hypothesis that Piezo1 is required for VF mucosal repair pathways of epithelial cell injury. Utilizing a sonic hedgehog (shh) Cre line for epithelial-specific ablation of Piezo1/2 mechanoreceptors, we investigated 6wk adult VF mucosa following naphthalene exposure for repair strategies at 1, 3, 7 and 14 days post-injury (dpi). PIEZO1 localized to differentiated apical epithelia and was paramount for epithelial remodeling events. Injury to wildtype epithelium was most appreciated at 3 dpi. Shhcre/+; Piezo1loxP/loxP, Piezo2 loxP/+ mutant epithelium exhibited severe cell/nuclear defects compared to injured controls. Conditional ablation of Piezo1 and/or Piezo2 to uninjured VF epithelium did not result in abnormal phenotypes across P0, P15 and 6wk postnatal stages compared to heterozygote and control tissue. Results demonstrate a role for Piezo1-expressing VF epithelia in regulating self-renewal via effects on p63 transcription and YAP subcellular translocation—altering cytokeratin differentiation.
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Introduction
The larynx, situated along the airway, is richly innervated with nerves [1, 2]. Diversity of physiological inputs from peripheral afferents in mucosa [3,4,5,6,7,8,9,10,11,12] serves to elicit characteristic reflexes to guard and/or clear the airways [13, 14]. Free margins of the vocal fold (VF), covered by stratified squamous epithelium (SSE), sustain extensive physiological stress during the biomechanical acts of voicing and cough [15,16,17,18,19,20,21]. Under homeostatic conditions, cells are largely quiescent, exhibiting low rates of proliferation and cellular turnover. However, when significant disruption occurs in response to injury and insult, rapid VF epithelium regeneration is critical for long-term tissue survival and maintenance. Analogous to other tissues, acute VF mucosal injury initiates a tissue repair process comprising of overlapping wound healing cellular phases involving: (1) inflammation, (2) proliferation and re-epithelialization and (3) ECM synthesis and remodeling [22,23,24,25]. VF mucosal epithelial cells, not only offer self-renewal capacity but, provide first-line defense against physical and chemical insult from the laryngeal lumen [17, 26, 27], requiring an intact protective barrier [15].
As few other organs and/or tissues withstand such shearing and frictional forces during motion [28], VF epithelial cells represent a unique cell type to study mechanotransduction. In 2010, a new group of mammalian mechanically activated ion channels, Piezo1 and Piezo2 were discovered [29], prompting a surge of research into their functional roles in cells. Since their discovery, Piezo1 channels have been found to be inherently mechanosensitive [30], predominantly expressed in nonexcitable cell types, and shown to regulate the life cycle of epithelial cells [31,32,33]. In contrast, Piezo2 channels have been found primarily expressed in sensory neurons [29, 34,35,36,37,38,39], regulating critical roles in sensory processes such as gentle touch sensation [40], proprioception [41] and mechanical nociception [42].
Given challenging biomechanical vibration within the larynx and subjected to VF tissue [18], it is not surprising that rapidly adapting mechanoreceptors have been implicated as functional afferents in laryngeal mucosa [10]. However, current research has relied on observational studies (e.g. punctate/air pulse mechanical stimulation) [43,44,45] and electrophysiological responses in vagal activity [46], with little underlying biological evidence at the mucosa–airway interface. Furthermore, PIEZO1/2 localization to laryngeal epithelial cells has not been explored, with initial evidence suggesting airway threats may act upon upstream epithelial sentinel cells rather than directly on neurons [13, 47].
The purpose of this study was to test the hypothesis that Piezo1-expressing ion channels to VF epithelia are essential for differentiation of SSE during postnatal life, and function to modulate acute remodeling following injury. We adopted a relatively established murine model for lower airway epithelial injury using chemical naphthalene [48,49,50,51,52,53,54]; not previously tested in the upper airway. Utilizing a sonic hedgehog (shh) Cre line for epithelial-specific ablation of Piezo1/2 mechanoreceptors, we investigated 6wk adult VF mucosa for repair strategies at 1, 3-, 7 and 14 days post-injury (dpi). We found that naphthalene facilitates VF mucosal injury with a noninvasive, reproducible and epithelial-targeted approach—most appreciated at 3 dpi in both wildtype and mutant colonies. Results demonstrated the role for Piezo1 in modulating self-renewal via effects on p63 transcription and YAP protein subcellular translocation. We noted irregular cytokeratin differentiation for downstream stratification events, most notably, ectopic K17 expression, with unique changes to the subglottis at the squamociliary junction. Regenerating epithelium was restored by 7 dpi for all genotypes and treatment conditions, albeit, by 14 dpi ZO1-marked tight junctions to apical cells were impaired for both uninjured and injured Piezo1 ablated epithelium, suggesting continued barrier compromise. Findings from this investigation improve our understanding of acute VF wound healing in the context of Piezo1 epithelial function and form the basis for an in vivo methodology to study VF responses to naphthalene injury.
Materials and methods
Mouse models and tissue harvesting
Shhgfpcre (JAX stock #05622), Piezo1flox (JAX stock #029213), Piezo2flox (JAX stock #027720) and Rosa26-CAG-loxp-stop-loxp-tdTomato (JAX stock #007914) were obtained from Jackson Laboratory. Data reported herein have been compiled from the examination of multiple embryonic and postnatal time points for wildtype (WT), heterozygous and conditional knockout animals. Both male and female mice were used in this study; experimental comparisons that underwent naphthalene injury controlled for sex. Pregnant dams and adults were killed for experiments through CO2 asphyxiation and cervical dislocation followed by isolation of vital postnatal organs and embryos. Fetal and neonate mice (< 10 days) were put on ice until motionless and euthanized via decapitation. Timed pregnancies were confirmed through visualization of vaginal plugs with noon on the day plugs were detected designated as E0.5. Mutant and control animals were studied at E15.5, E18.5, P0, P15 and 6 weeks of age. Mice were bred and housed in the University of Wisconsin-Madison Biomedical Research Model Services Laboratory. Animal procedures were approved by the University of Wisconsin-Madison Institutional Animal Care and Use Committee and conducted in accordance with the National Institutes of Health Guide for the Care and Use of Laboratory Animals [55].
Generation of transgenic and mutant mouse models
All offspring were genotyped using primer pairs shown in Table S1. Shhcre/+ mice were maintained by crossing male heterozygotes (Shhcre/+) with WT female littermates on a C57BL/6 J background. Mice maintained on a B6 background were used as WT controls for immunofluorescence (IF) experiments to label endogenous PIEZO protein expression patterns. Shhcre/+ mice exhibited no abnormal phenotypes and were identical to WT colony controls. Shhcre/+ males were then crossed to ROSA26CAG−LSL−tdTom/tdTom females to generate Shhcre/+; ROSA26CAG−LSL−tdTom/+ (here referred to as ROSALSL−tdTom/+) mutants. ROSAtdTom/+ controls were used to establish the baseline tdTomato levels for all experiments. Shhcre/+; Piezo1loxP/+, Piezo2loxP/+ males were crossed to Piezo1loxP/loxP, Piezo2loxP/loxP females to generate conditional Shhcre/+; Piezo1loxP/loxP, Piezo2loxP/loxP compound (here referred to as Shhcre/+; Piezo1/2cko) and single mutants (here referred to as Shhcre/+; Piezo1cko, Piezo2loxP/+ or Shhcre/+; Piezo1loxP/+, Piezo2cko). Shhcre/+; Piezo1loxP/+, Piezo2loxP/+ (here referred to as Shhcre/+; Piezo1/2loxP/+) and mice without the Cre allele insertion were used as littermate genotype controls. Mice lacking Cre recombination with floxed Piezo1/2 insertions exhibited similar epithelium morphology to WT colony controls.
Histology and immunofluorescence
Larynges and control tissues were collected at the indicated ages or timepoints following confirmation of vaginal plug and/or naphthalene administration. For embryos and P0 pups, mouse neck regions were dissected, with isolation of the entire larynx for samples > P0. All samples were immediately fixed in 4% paraformaldehyde in phosphate-buffered saline at 4 °C overnight. Samples were subsequently dehydrated in a gradient series of ethanol, treated with xylene and embedded in paraffin wax. Paraffin wax blocks were cut into serial Sects. (5 µm), dewaxed and rehydrated, heated to boiling in 10 mM citrate buffer (pH 6 or pH 9) for antigen retrieval and treated with 0.5% Triton X-100 in PBS. Sections were then stained using a standard IF protocol [56]. IF staining for PIEZO2 proteins were performed using a direct Tyramide SuperBoost™ Signal Amplification (TSA) kit (B40943, Invitrogen) followed by TrueBlack® Lipofuscin Autofluorescence Quencher (Biotium) staining following product’s published protocols. Cerebral cortical/cerebellar brain and dorsal root ganglion (DRG) tissues were isolated for positive controls for PIEZO1 and PIEZO2 antiserum, respectively (Fig. S1). Negative controls for PIEZO antibodies included antiserum + KO, antiserum + block peptide, in addition to, no applied primary antiserum. All primary and secondary antibodies used are listed in Table S2. Primary antibodies were applied overnight at 4 °C. Peptide block serum was mixed with PIEZO antibodies at 10-to-1 concentration ratio, preincubated for 60 min with rotation and then solution was applied overnight at 4 °C. Sections were incubated with DAPI (1:3000 ratio) for 10 min at RT. Slides were mounted and coverslipped with Prolong Diamond mounting media (Fisher P36970), cured flat at room temperature in the dark for 24 h, and stored at 4 °C. Each experiment was replicated at least twice for all timepoints and targets assessed.
RNA isolation, cDNA generation and qRT-PCR
Dissected larynges from control and experimental mutants at P0 timepoint were used for RNA isolation using ReliaPrepTM RNA Tissue Miniprep System (Promega, Madison, WI, USA) following manufacturer’s protocols. For Piezo1 and Piezo2 mRNA transcript analysis at P0 timepoint, each control and experimental group consisted of 5 pooled larynges to maximize total RNA yield for downstream qPCR experiments. For all mRNA transcript analyses at 6wk timepoint, each control and experimental group consisted of 3 pooled larynges. One thousand ng of RNA was reverse transcribed to cDNA using reverse transcription reagents (Go Script, Promega) per manufacturer’s protocol. Expression levels of selected genes were analyzed with qPCR (Table S1). Total volume of 0.4 μl of cDNA was used per 20 μl real time qPCR reaction using Power Up Sybr Green Master Mix (Applied Biosystem, Foster City, CA, USA) and run for 40 cycles in triplicates on a 7500 Fast Real Time PCR System machine (Applied Biosystem), according to manufacturer’s instructions. Relative gene expression between control and experimental mutant larynges were normalized to β-actin (ΔCt) and control tissue (ΔΔCt).
Naphthalene treatment
Adult 6wk mice received 0.275 mg/per gram body weight of Naphthalene (Millipore Sigma, cat# PHR1275-1G) administered with an insulin syringe 29-gauge needle dissolved in corn oil (Sigma, cat# C8267) interperitoneally. Corn oil alone injected littermates were used as control comparisons for each experimental condition with analysis at 1-, 3-, 7- and 14-dpi.
Image acquisition
Images were acquired on a Nikon Eclipse Ti2 inverted microscope (Nikon Instruments, Inc., Belmont, CA, USA) with Nikon DS-Ri2 camera and NIS Elements software (version 5.21.01). Images were uniformly adjusted for brightness and exposure using Nikon or ImageJ software. IF staining presence, relative intensity, and subcellular and tissue-level localization were described qualitatively. Sections from experimental groups were imaged using the same exposure settings or laser power for any given antibody combination. All schematic images were created in Procreate (version 5.2.2) or BioRender.com.
Cell count quantification
Epithelial cell counts were quantified with 40X magnification from 10 images across the length of VF ≥ 20 um apart. Images of either EdU+/H2AX+, P63+/SCGB1A1+ or P63+YAP+ stained tissues were opened in ImageJ (National Institutes of Health). Total epithelial cells, as well as EdU+ and P63+ labeled cells were enumerated using an automated counting macro “RGB fluorescent cell count v1.32.ijm” developed by Dr. David Ornelles (Wake Forest University, Winston-Salem, NC) as previously described [57, 58]. Prior to using the macro for these experiments, it was tested in comparison to manual cell counts of 40X images of VF sections from 4 serial EdU+ and P63+ stained slides. H2AX+ and SCGB1A1+ cells as well as YAP+ nuclei were manually counted using the built-in cell counter in ImageJ. Cytologically visible H2AX foci indicate DNA double-stranded breaks [59], with nuclei containing greater than 50% or more total area of H2AX foci considered positive.
Statistical analysis
All data were tested for normality and equal variance using the Shapiro–Wilks and F test, respectively. A Wilcoxon rank sum test was performed to calculate significance in mRNA abundance transcript levels. To analyze WT tissue, total epithelial cell counts (DAPI) between corn oil control and naphthalene-injured VF epithelium were analyzed for comparison at each timepoint of interest (1-, 3-, 7-, 14-dpi). Proliferation and apoptosis (percentages of EdU+ cells and H2AX+ cells) as well as cell-marker indices (percentages of SCGBA1A+ cells, P63+ and YAP+ nuclei) were normalized to total DAPI+ cells for comparisons at 3 dpi timepoint. To analyze Piezo1 allele dose effect in heterozygote versus complete knockout, P63+ and YAP+ nuclei were compared using the Wilcoxon rank sum test. One-way analysis of variance (ANOVA) was used to compare group means for relative mRNA abundance of P63+, YAP1+ and K13+ and K17+ transcripts with Tukey’s HSD post hoc test to determine pairwise comparisons. Kruskal–Wallis test was used to compare group means for relative mRNA abundance of YAP2+. All statistical analyses were calculated with RStudio (v. 2021.09.0 Build 351) running R 4.1.2 (R Core Team). Results are reported as either mean ± SE or median (Q2) with lower (Q1) and upper (Q3) quartile ranges. The alpha level for significance was p ≤ 0.05.
Results
PIEZO proteins exhibit selective expression in laryngeal epithelia
To investigate how ablation of Piezo1 and Piezo2 disrupts VF epithelia, we first assessed PIEZO1 and PIEZO2 endogenous protein expression in the larynx and VF on the background of WT control mice (Fig. 1). Two regions selected for investigation were the mid-membranous and cartilaginous arytenoid VF, known for roles in tissue oscillation [60,61,62] and airway protection [43, 63], respectively (Figs. 1a; 2c). Within the larynx of P0 WT mice, we detected PIEZO1 to nonkeratinized SSE of the mid-membranous and ventral VF (Fig. 1b), and keratinized SSE of the hypopharynx and esophagus (Fig. 1b; Fig. S1). PIEZO2 expression was selective to ciliated, respiratory epithelia of the hypopharynx, aryepiglottic fold, superior surface of arytenoid, inferior laryngeal epiglottis and trachea at PO, however, additionally extended into the subglottic region by 6wks (Fig. 1b). PIEZO1 expression at the 6wk timepoint was localized to apical, differentiated squamous epithelia of the VF (Figs. 1b; 2d). We next determined de novo epithelial expression of PIEZO1 and PIEZO2 proteins by staining embryonic tissue at timepoints preceding and subsequent to VF recanalization. We established PIEZO1/2 de novo epithelial cell expression patterns shortly after VF recanalization (E16.5-E17.5), denoted by no expression at E15.5, and with peak expression at E18.5 and P0 (Figs. S2&S3). We further demonstrated PIEZO2 expression abuts PGP9.5 labeled neurons in the posterior glottis and near nerve endings arborizing in the supra- and subglottis at P0 (Fig. S4).
Shh cre/+; Piezo1/2 cko mutants develop normal VF epithelial architecture by 6wks
To determine the effect of Piezo removal specifically from shh-positive airway epithelial cells, we generated triple transgenic mice carrying the sonic hedgehog (shh) Cre, floxed Piezo1 and floxed Piezo2 alleles, referred to as Shhcre/+; Piezo1/2cko throughout the text (Fig. 2a, b). We first verified that Cre recombinase was expressed exclusively in VF epithelial cells using a tdTomato reporter (Shhcre/+; ROSALSL−tdTom/+) which expresses a red fluorescent protein (RFP) following Cre recombinase (Fig. 2a and e). ROSALSL−tdTom/+ and no primary negative controls were used to establish baseline tdTomato levels and effective antiserum against RFP, respectively (Fig. S5). We then harvested P0 whole larynges from control and Shhcre/+;Piezo1/2cko to verify effective Piezo1/2 epithelial cell ablation, which revealed significantly reduced mRNA for both Piezo1 (W = 186, p = 0.0001) and Piezo2 (W = 174, p = 0.0019) (Fig. 2f). Immunofluorescence analysis validated reduced protein expression following Piezo1 genetic ablation (Fig. S6a). Mutant combinations at P0, P15 and 6wk timepoints were collected to establish morphology following Piezo1 and/or Piezo2 loss. To our surprise, no epithelial morphological differences were appreciated to the mid-membranous VF across all mutant combinations at P0, P15 (Fig. S7) and/or 6wk stages (Fig. 2g). IF analysis confirmed normal cytokeratin differentiation expression of SSE markers K13 and K14 in Shhcre/+; Piezo1/2cko compound mutant compared to control tissue (Fig. 2h), albeit, downregulated ZO1 to apical epithelium following Piezo ablation (Fig. 2i). To further investigate epithelial structural changes, we stained for MUC1, an important mucin previously characterized in human VF epithelium [64]. Interestingly, we found overexpression of MUC1 following Piezo1 deletion, which suggests the VF epithelium compensates via downregulating ZO1 and upregulating MUC1 production ultimately priming signaling cascades for repair strategies (Fig. S8).
NAPH-induced injury results in hyperplasia to WT VF epithelium
NAPH exposure in mice results in airway epithelium-specific injury, which has proven valuable for study of regeneration in distal lung and tracheal airways [48,49,50,51, 65, 66]. Adult 6wk control, Shhcre/+;Piezo1/2loxP/+ haploinsufficient and Shhcre/+;Piezo1cko,Piezo2loxP/+ knockout animals were treated with NAPH or vehicle-treated (corn oil) control and sacrificed at 1-, 3-, 7-, and 14-dpi (Fig. 3a). To test feasibility of NAPH chemical exposure to result in VF epithelial injury, WT controls were used in our initial analyses. No epithelial injury was observed in WT controls on day 1 following NAPH exposure, however, by 3 dpi, WT VF epithelium exhibited hyperplasia with increased stratification of squamous cells and with significantly increased total epithelial cells compared to corn oil uninjured controls (173 ± 45 corn vs. 242 ± 52 naph, p < 0.0001) (Fig. 3b, c). This increased stratification and resulting hyperplasia was noted across the full length of the VF (Fig. 3d). IF analysis corroborated histological findings, demonstrating increased EdU+ epithelia (1.71 ± 1.35 corn vs. 6.56 ± 5.01 naph, p < 0.0001) and H2AX+ epithelia (0.67 ± 0.92 corn vs. 1.74 ± 1.08 naph, p < 0.0001), compared to controls at 3 dpi only (Fig. 3e). Hyperplastic changes were largely resolved by 7 dpi (Fig. 3b), with no differences in epithelial cell counts (148 ± 26 corn vs. 154 ± 31 naph, p = 0.1988) (Fig. 3c). Abnormal cell morphology was localized to the VF subglottic region (Fig. 3d), which was defined as below the point where squamous epithelium of the VF reaches its inferior extent (a.k.a. squamociliary junction). Further characterization of serial sections revealed increased EdU+ epithelia along the dorsal–ventral plane to the arytenoid and mid-membranous regions compared to ventral sections (Fig. 3g), with increased concentrated labeling of EdU + cell proliferation to the subglottic region of the mid-membranous fold (Fig. 3h).
To determine potential mechanism for NAPH-induced epithelial injury and resulting hyperplasia, VF sections were stained for P63 and SCGB1A1, indices for basal cell progenitor and secretory cells, respectively. NAPH-injured WT VF epithelium exhibited expansion to P63+ cells within the basal compartment (Fig. 4a). Cell count quantification revealed significantly increased P63+ cells following NAPH injury at 3 dpi only (58.0 ± 8.14 corn vs. 70.2 ± 9.85 naph, p < 0.0001) (Fig. 4b). IF analysis also exhibited ablation of SCGB1A1+ secretory cells to the mid-membranous fold at 3 dpi compared to corn oil controls (Fig. 4a). SCGB1A1+ secretory cells were demonstrated across the full length of VF in WT corn oil controls in both squamous and cuboidal epithelia (Fig. 4e). Furthermore, quantification comparing injured to uninjured controls at each timepoint revealed transient epithelial sensitivity to NAPH with significant cell decrease by 1 dpi (3.70 ± 1.79 corn vs. 2.21 ± 1.89 naph, p = 0.003), significant near complete ablation by 3 dpi (4.33 ± 2.61 corn vs. 0.21 ± 0.49 naph, p < 0.0001), albeit, population rebound by 7 dpi (4.67 ± 2.45 corn vs. 4.54 ± 3.19 naph, p = 0.8608) and no significance differences at either 7 or 14 dpi (3.78 ± 1.9 corn vs. 2.83 ± 1.93 naph, p = 0.0643) (Fig. 4c). Lastly, we stained markers for K13 and K14, known to be important for maturation and differentiation of murine SSE [26, 56, 67], along with the stress cytokeratin marker K17. Most interestingly, expression of K13 extended into the subglottic region with apparent positivity to non-squamous epithelia at the squamociliary junction (Fig. 4f), with K14 overexpression extending into the tracheal lower airway following NAPH injury compared to controls (Fig. S9). No K17 expression was noted in either treatment group (Fig. 4f).
Piezo1 ablation results in increasingly severe VF epithelial defects
We hypothesized that following NAPH insult and loss of Piezo1 to apical VF epithelial cells that we would observe epithelial remodeling defects. Given data from WT controls, we expected severe injury to occur by 3 dpi. Consistent with our hypothesis, we noted a Piezo1 allele dose effect such that following Piezo1 loss mutant epithelium exhibited increasingly severe cell defects compared to NAPH-treated control and/or Shhcre/+;Piezo1/2loxP/+ heterozygotes (Fig. 5a–c). Phenotypic abnormalities to Shhcre/+;Piezo1cko,Piezo2loxP/+ mutants were characterized by loss of stratification, disorganized/large squamous cells with polymorphous nuclei, irregular nuclear borders and large amounts of cytoplasm with indistinct cell borders (Fig. 5b, c). Epithelium defects across all genotype conditions were resolved by 7 dpi with no differences to corn oil controls, characterized by re-epithelization and complete restoration (Fig. 6a–c).
Piezo1 modulates self-renewal via repressive effects on p63 and YAP subcellular localization
Previous findings from our lab have demonstrated the importance of Hippo/YAP pathway for VF epithelium morphogenesis [68]. Moreover, Piezo1 and Piezo2 have been implicated as upstream components for signal transduction pathways resulting in YAP transcriptional activity for stem cell differentiation [69]. Mice were investigated at 3dpi due to the appreciated phenotypic differences (Fig. 7a). Adult VF epithelium expresses mainly cytoplasmic YAP with very few cells exhibiting nuclear expression mainly localized to apical cells (Fig. 7b) and indicating a role in epithelial cell stratification [68]. Prior work has also revealed a role for both P63 and YAP in epidermal basal SC proliferation and epithelial stratification [70,71,72,73], with additional evidence implicating their genetic interaction in the airway epithelium [74]. We reasoned that injury to the VF may result in increased YAP+P63+ epithelial cells with nuclear YAP translocation in our controls which exhibited hyperplasia and increased keratin expression. We also hypothesized analogous loss of YAP expression following Piezo1 loss-of-function in our mutant epithelium given prior work [69, 75]. IF analysis revealed upregulated YAP in control epithelium following NAPH injury at 3 dpi, albeit, with grossly cytoplasmic expression localized to the basal cell compartment (Fig. 7c, d). More intriguing was the translocation to nuclear YAP expression in Shhcre/+;Piezo1/2loxP/+ and Shhcre/+;Piezo1cko,Piezo2loxP/+ injured mutant VF epithelium (Fig. 7c, d). We also appreciated increased co-localization of nuclear P63 and YAP expression in Shhcre/+;Piezo1cko,Piezo2loxP/+ mutant VF epithelia (Fig. 7c, d). Cell count quantification revealed significantly increased nuclear YAP+ (7.87 ± 5.78 Shhcre/+;Piezo1/2loxP/+ vs. 22.1 ± 10.2 Shhcre/+;Piezo1cko,Piezo2loxP/+, p < 0.0001) and YAP+P63+ (4.72 ± 3.97 Shhcre/+;Piezo1/2loxP/+ vs. 10.4 ± 7.46 Shhcre/+;Piezo1cko,Piezo2loxP/+, p = 0.002) VF epithelia following Piezo1 loss in NAPH-exposed groups (Fig. 7e). Additionally, we performed quantitative-PCR analysis for p63, Yap1, and Yap2 transcripts to assess mRNA abundance across genotype comparisons. We found p63 mRNA exhibited an inverse relationship following Piezo1 loss in NAPH-exposed groups, such that p63 expression increased in a linear manner from control to Shhcre/+;Piezo1cko,Piezo2loxP/+ mutant VF epithelium (Fig. 7f). One-way ANOVA test revealed significant group differences (F(2,6) = 101, p < 0.0001), with Tukey-adjusted HSD post hoc tests exhibiting significantly different p63 mRNA expression (p < 0.0001) for all genotype pairwise comparisons. This supports Shhcre/+;Piezo1cko,Piezo2loxP/+ histologic findings of nuclear hypertrophy, with IF analysis corroborating more robust P63 nuclear expression following Piezo1 loss compared to heterozygote epithelium (Fig. 7g). There were no group differences in Yap1 (F(2,6) = 2.607, p = 0.153) and/or Yap2 (H(2) = 4.3556, p = 0.1133) mRNA abundance across genotype conditions (Fig. 7f). While overall Yap mRNA transcript levels are unchanged, the clear shift from cytoplasmic-to-nuclear YAP protein localization suggests posttranslational modification, which may have a functional effect on p63 resulting in appreciated basal progenitor cell proliferation and epithelial stratification events.
Piezo1 loss alters differentiation programs for VF remodeling events
K17, not normally expressed in healthy VF epithelium, is known for its role in wound healing and DNA damage [76,77,78]. Prior work has also established reciprocal effects of K17 emergence with K13 loss in head and neck squamous cell carcinoma (SCC) [79,80,81]. We therefore postulated that aberrant K13/K17 expression may be partly contributing to abnormal stratification events appreciated in our Piezo1 ablated epithelium. To test this, we stained control, Shhcre/+;Piezo1/2loxP/+ and Shhcre/+;Piezo1cko,Piezo2loxP/+ mutant tissue at 3 dpi following NAPH or corn oil exposure (Fig. 8a). K13/K17 expression patterns were consistent with prior characterization data such that K13 was increased after injury localized to apical VF epithelia with a lack of K17 expression to VF epithelium in either treatment condition (Fig. 4f; Fig. 8b, c). Interestingly, K17 expression was exhibited to the subglottis at the squamociliary junction in corn oil uninjured Shhcre/+;Piezo1/2loxP/+ mutant epithelium increasing to surrounding VF SSE following NAPH injury (Fig. 8d). Shhcre/+;Piezo1/2loxP/+ mutant larynx displayed preferential K17 expression to squamous epithelia of the VF, as well as squamous and intermediate epithelia of the laryngeal surface of the epiglottis (Fig. S10). Further investigation revealed what seemed to be reciprocal expression patterns in Shhcre/+;Piezo1/2loxP/+ haploinsufficient and Shhcre/+;Piezo1cko,Piezo2loxP/+ mutant VF epithelium following injury, whereas loss of K13+ epithelia resulted in increased ectopic K17+ epithelia (Fig. 8c). However, quantitative-PCR analysis confirmed no difference for K13 mRNA across genotype conditions (F(2,6) = 0.63, p = 0.565), albeit, group differences were noted comparing K17 mRNA abundance across conditions (F(2,6) = 20.168, p = 0.002). Tukey-adjusted post hoc pairwise tests revealed significantly increased K17 mRNA comparing controls to Shhcre/+;Piezo1/2loxP/+ (p = 0.0039) and Shhcre/+;Piezo1cko,Piezo2loxP/+ (p = 0.0034) mutants following NAPH injury (Fig. 8e). To further explore if aberrant K13/K17 differentiation was related to beginning stages for squamous cell metaplasia, we stained for SNAIL1 and N-cadherin [82,83,84]. Results were negative for both markers in Shhcre/+;Piezo1cko,Piezo2loxP/+ mutant epithelium ruling out the possibility of epithelial-to-mesenchymal transition (EMT) (Fig. 8f).
Barrier integrity is compromised up to 14 dpi in Shh cre/+ ;Piezo1 cko ,Piezo2 loxP/+ mutant VF epithelium
Damage to the epithelial stratum results in barrier function compromise, associated with acute phases of VF injury and ensuring tissue remodeling [15, 17, 25, 85,86,87,88,89]. We performed IF analysis at 3- and 14-dpi for adheren junction marker, E-cadherin and tight-junction marker, ZO1. At 3 dpi, we found normal E-cadherin cell–cell expression with localized ZO1 expression to suprabasal epithelia in corn oil controls (Fig. 9). However, NAPH-injured WT VF exhibited increased E-cadherin and ZO1 to hyperplastic epithelium with mislocalized ZO1 penetrating to the basal cell layer (Fig. 9). As expected, NAPH-injured Shhcre/+;Piezo1cko,Piezo2loxP/+ mutant VF epithelium displayed discontiguous expression patterns of E-cadherin and ZO1 resulting from epithelium defects at 3 dpi. Interestingly, ZO1 discontiguous expression was also noted in corn oil treated Shhcre/+;Piezo1cko,Piezo2loxP/+ control epithelium (Fig. 9). By 14 dpi, corn oil control and NAPH-injured Shhcre/+;Piezo1cko,Piezo2loxP/+ mutant VF epithelium continued to exhibit discontiguity of ZO1 expression to apical epithelia, suggesting continued structural compromise to restored epithelium (Fig. 9).
Discussion
We have leveraged the recent discovery of Piezo1 [29], a mechanosensitive channel known to regulate the life cycle of epithelial cells [31,32,33], to test the hypothesis that Piezo1 is a critical mechanoprotein for VF SSE and repair pathways for acute VF remodeling events. PIEZO expression displayed selectivity to distinct laryngeal epithelia, such that PIEZO1 was expressed to differentiated, apical SSE of the VF, hypopharynx and esophagus, while PIEZO2 expression was restricted to respiratory and intermediate epithelia of supra- and subglottic regions. Cell expression patterns are likely the result of evolved function for various airway epithelium. Human studies have shown that the mid-membranous VF is largely unresponsive to stimulation of the mucosa, suggesting lack of laryngeal adductor reflex (LAR) sensory receptors, and a physiological advantage due to the high biomechanical loads endured during phonation [43, 63]. On the contrary, PIEZO2-expressing epithelia predominated in LAR sensory hotspots [1, 43, 63, 90, 91], and abutted PGP9.5-labeled arborizing nerve fibers (Fig. S4), suggesting a possible role for laryngeal mechanosensation. Direct links to Piezo2 mechanosensory function in the larynx, however, has not been established. Contrary to expectations, Piezo1 was dispensable for proper specification and maturation of developing VF epithelium with normal histology and cytokeratin differentiation in Shhcre/+;Piezo1/2cko compound mutant epithelium at the 6wk timepoint. We suspect that during normal development and epithelial homeostasis of the murine VF that the mechanical environment is not elevated enough to result in Piezo1 activation, thus lacking aberrant pathway signaling following Piezo1 loss. While previous evidence suggests that mice are capable of VF vibration [92,93,94], the primary mode of vocal communication is done using ultrasonic vocalization (USV), which is produced in the larynx [95,96,97]. Audible cries, also attributed to VF vibration, is produced in laboratory mice, albeit, to a limited extend with USV predominating for close-distance mating and social contexts [95]. Given the lack of impact collisional forces during VF vibration in these settings, we suspect the mechanical challenge to VF mucosa is quite low in laboratory-housed mice. In other words, the reduced biomechanical challenge to VF mucosa in laboratory-housed mice may explain why we see limited Piezo1 localized expression in sparse epithelia of the apical layer and not more widespread throughout the epithelium. The opposite could also be argued, such that Piezo1 expression may be limited given the repetitive biomechanical demands to murine VF mucosa. Recent work may support the latter notion, indicating transduction limits of Piezo channels, which turn out to be poor discriminators and inefficient transducers of continuous high-frequency stimulations in other organs/cell types [98]. It will be of interest for future work to investigate Piezo1 in human VF mucosa to identify species similarities/comparisons and functional roles for translational impact.
VF epithelia are short-lived and undergo constant turnover and renewal throughout life. In addition, epithelial cells are the initial cell type in the VF to encounter toxic exposure, which may make them principal modulators of VF disease development [15, 17]. We utilized the chemical toxin naphthalene, commonly found in a variety of pollutants/irritants (wood smoke, tar, asphalt, fossil fuels), and inhaled carcinogens (tobacco smoke) [48, 52, 99], to injure VF mucosa which specifically targets damage to epithelial cells via its effect on the cytochrome P450 system [48,49,50,51,52,53,54]. To assess Piezo1-mediated repair pathways, adult 6wk control and mutant VF epithelium underwent injury for analysis at 1-, 3-, 7- and 14-dpi. NAPH exposure to WT VF epithelium resulted in appreciated injury at 3 dpi, characterized by epithelial hyperplasia along with increased EdU+ cell proliferation and H2AX+ DNA damage. We also demonstrated that NAPH injury resulted in P63+ basal cell compartment expansion with transient sensitivity to SCGB1A1+ epithelia, denoted by near complete ablation at 3 dpi with population rebound by 7 dpi. Our data confirm previous molecular findings regarding NAPH effect on airway epithelium, albeit, with varying rates of cellular injury and repair [48, 50, 65].
We then hypothesized that injury to VF mutant epithelium would mimic an elevated mechanical environment, ultimately surpassing Piezo1 channel thresholds, for activation of aberrant downstream pathways following Piezo1 loss. The basis of this assumption was that Piezo1- expressing VF epithelial ion channels are likely of the high-threshold type that only respond to intense mechanical stimuli and/or injury [100]. Consistent with our hypothesis, NAPH-exposure in Piezo1 mutant epithelium resulted in increasingly severe VF injury at 3 dpi. Specifically, Shhcre/+;Piezo1cko,Piezo2loxP/+ VF epithelial defects were characterized by loss of stratification, disorganized/large squamous cells with polymorphous nuclei, irregular nuclear borders, and large amounts of cytoplasm with indistinct cell borders. Consistent with other work in rodent VF injury models [25, 85,86,87,88,89], epithelium regeneration and structural restoration was appreciated by 7 dpi in WT control and mutant colonies. Previous in vitro work has shown the role of mechanosensitive channels as primary transducers in force transmission to the nucleus [101], with shear stress-induced nuclear shrinkage through activation of Piezo1 channels in epithelial cells [102]. Our in vivo data present related findings, such that Piezo1 epithelial loss appeared to modulate and enlarge nuclear size, however, whether this results from lack of Ca2+ signaling and/or direct mechanical interactions of actin with the nuclear membrane remains unknown.
Next, we turned our attention to investigating YAP expression given the importance of Hippo/YAP pathway for VF epithelium morphogenesis [68], and previous work implicating Piezo1 as an upstream component for YAP transduction pathways [69, 75]. Shhcre/+;Piezo1/2loxP/+ and Shhcre/+;Piezo1cko,Piezo2loxP/+ mutant VF epithelium revealed a clear shift from cytoplasmic-to-nuclear YAP expression compared to control following NAPH injury. In addition, increased YAP+P63+ cell co-localization was exhibited following Piezo1 ablation compared to heterozygote epithelium. qPCR revealed significantly increased p63 mRNA abundance in NAPH-exposed Shhcre/+;Piezo1cko,Piezo2loxP/+ mutant compared to heterozygote and control epithelium, albeit, no differences were noted in either Yap1 and/or Yap2 mRNA transcripts. These effects, following Piezo1 ablation, suggest posttranslational modification of YAP facilitating subcellular localization, which may have a direct effect on p63 resulting in epithelial cell proliferation and stratification defects. These data support prior work that has demonstrated genetic interaction of p63 and Yap in airway epithelium [74]; with other known roles for epithelial proliferation and stratification events in skin [70,71,72,73].
To assess downstream cell signaling events for SSE remodeling, we stained cytokeratin differentiation markers K13 and K14, as well as K17—a marker known to be upregulated during wounding [77] and DNA damage [76]. K14 has been shown preferentially located to basal VF epithelia with diffusion across all cell layers [56, 64], believed to play a pivotal role in cell shape and resistance to mechanical trauma in mouse epidermis [78]. Our data confirm previous work with increased K14 expression across all layers of WT VF epithelium at 3 dpi, extending into distal trachea to K14+ basal cells and submucosal compartments. In addition, K13, a marker of differentiated SSE, was overexpressed with noted ectopic positivity to non-squamous cells at the squamociliary junction of the subglottis. K17, not normally expressed in healthy VF epithelium, exhibited no expression to either WT uninjured and/or injured epithelium. Together, data suggest predilection for unique cellular changes to the laryngeal subglottic region following insult supporting vulnerability at epithelium transition zones in the larynx [103,104,105]. Further work is warranted to establish whether these cells hold any particular significance for VF barrier function and immunomodulation.
Elevated expression of K17 has also been shown to play a role in head and neck SCC associated with decreased survival rates [79,80,81], in addition to its role in mouse papillomavirus [57]. Interestingly, Shhcre/+;Piezo1/2loxP/+ mutant epithelium exhibited ectopic K17 focal emergence to the squamociliary junction in uninjured corn oil controls, extending to SSE of the VF and laryngeal surface of the epiglottis following NAPH injury. Emergence of K17 with analogous loss of K13 has been found associated with more invasive malignant disease in oral SCC [79]. NAPH-exposed Shhcre/+; Piezo1/2loxP/+ and Shhcre/+; Piezo1cko, Piezo2loxP/+ mutant epithelium revealed what seemed to be reciprocal expression patterns for K13/K17, such that ectopic K17 expression to VF epithelial cells led to an inverse loss of K13 expression. qPCR analysis confirmed significantly increased K17 mRNA fold change comparing Piezo1 mutant epithelium to control, albeit, no K13 differences were exhibited. Researchers have hypothesized that K17 may serve as an early marker for epithelial transformation [81], therefore, we stained for N-cadherin and SNAIL1, known markers for EMT during gastrulation and tumorigenesis [106, 107]. Results were negative, ruling out the possibility of EMT to VF epithelium following Piezo1 loss with combined NAPH injury. While our data suggest possible links between Piezo1 signaling in K13-expressing VF apical epithelia and aberrant K17 expression, downstream pathways remain unclear with further work warranted.
Lastly, we were interested in long-term remodeling effects to VF barrier integrity following Piezo1 loss. SSE provides the VF with a protective barrier against mechanical, chemical and biological insult. Two well-known proteins important for maintaining cell–cell adhesion in SSE include E-cadherin and ZO1, markers of adheren and tight junctions, respectively. By 3 dpi, WT NAPH-injured controls demonstrated excessive E-cadherin and ZO1 proteins, with mislocalized ZO1 penetrating to basal stratum compared to corn oil controls. By 3 dpi, NAPH-exposed Shhcre/+;Piezo1cko,Piezo2loxP/+ mutant epithelium exhibited disrupted E-cadherin and ZO1 proteins. Interestingly, fragmented ZO1 expression was displayed along the apical edge of corn oil uninjured Shhcre/+;Piezo1cko,Piezo2loxP/+ mutant epithelium. By 14 dpi, Shhcre/+;Piezo1cko,Piezo2loxP/+ mutant epithelium continued to exhibit compromise to barrier integrity by fragmented ZO1 expression in both corn oil-uninjured and NAPH-injured treatment conditions. This suggests that despite structural epithelial restoration by 7 dpi, barrier integrity may be delayed up to 14 days or longer following injury within an epithelium that lacks Piezo1 epithelial cell ion channels. Ongoing compromise of regenerated epithelium implies that VFs may remain at risk for recurrent damage, pathogen invasion and remodeling. More long-term studies for Piezo1 importance to VF barrier function following injury are required in both animal and in vitro human air–liquid interface model systems for comparative analysis and translational impact.
A few limitations of the present work need to be addressed. First, while experiments explored mechanistic insight by which Piezo1 functions to regulate VF epithelium, inherent genetic redundancy of Piezo mechanoreceptors to tissue- and cell-function exist [108, 109]. This effect is assumed across many upstream transduction mechanisms [110], enacting safeguards for continued cell function and repair strategies. We accounted for this scenario by creating double mutants for Piezo1 and Piezo2 null alleles from the shh promotor. This, however, both extended our study for mutant tissue collection and limited our total number for investigative analyses. We found Piezo1, only, to be important for VF epithelium. To this end, we did not appreciate a phenotype without concurrent injury, thus, we cannot rule out that this may be due to inefficient complete genetic Piezo1 deletion. Residual Piezo1 expression may have been sufficient to prevent aberrant epithelial signaling, albeit, with injury this threshold may have been surpassed leading to our phenotype. Another limitation was that NAPH-induced injury was not 100% reproducible. Visual examination of WT histologic samples exhibited a 60% success rate for VF injury at 3 dpi (n = 16) with a 100% survival rate by 14 dpi (n = 5). Evidence suggests dose concentration [111], time of dose (circadian rhythms) [112], murine background strain [113], sex [51] and likely host immune response all contribute to variability in injury uptake and severity. Prior research in the lung and tracheal airways has utilized intraperitoneal dose concentrations ranging from 200 to 300 mg/kg [48,49,50,51, 65, 66, 111]. We chose a higher dose of 275 mg/kg given less concentrated secretory cells in the larynx and work suggesting higher doses are required for injury in proximal lung regions. For our intent purposes, we were able to use the proximal tracheal airway as an internal control for injury uptake at earlier timepoint analyses (1 & 3 dpi) due to the large effect on concentrated club cells in tracheal epithelium, especially at this higher dose. Furthermore, on account of our initial data we mainly focused our efforts on characterizing injury at day 3 following NAPH exposure. A thorough examination of all time points across wound healing phases is necessary to elucidate VF remodeling events in a murine model of regeneration.
Taken together, findings from this investigation improve our understanding of acute VF wound healing in the context of Piezo1 epithelial function and form the basis for an in vivo methodology to study VF responses to NAPH injury. In vitro model systems with Piezo1 gain-of- and loss-of-function are warranted to further clarify and refine the mechanism of Piezo1 action in VF regeneration and remodeling following NAPH exposure. In addition, use of pharmacological agents, such as Piezo1 agonists (Yoda1, Jedi1/2) and/or antagonists (Dooku1, Gadolinium, GsMTx4) would provide evidence for a potential use as therapeutic interventions. Recent interest has been given to the emerging role of Piezo1 in meningeal nociception leading to migraine headache, [114, 115] with speculation into roles for modulating/mitigating VF mucosal injury. We conclude that Piezo1 mechanosensitive ion channels play a crucial role in VF regeneration and remodeling, likely through regulating epithelial self-renewal via effects on p63 transcription and YAP subcellular localization—altering cytokeratin differentiation (Fig. 10).
Data availability
The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.
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Acknowledgements
We gratefully acknowledge Sierra Raglin for her expert assistance with tissue sample preparation, David Yang for help with histopathology, Julianna Tibbetts for assistance with genotyping and Xin Sun for helpful discussions.
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This work was supported by grants NIH NIDCD R01 DC04336, NIDCD R01 012773 and F31 DC018978.
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All authors contributed to the study conception and design. Material preparation, data collection and analysis was performed by AGF. Writing—original draft preparation: AGF. Writing—review and editing: AGF and SLT. All authors read and approved the final manuscript.
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All procedures performed in the study were in accordance with the University of Wisconsin-Madison Biomedical Research Model Services Laboratory (BRMS). The ethical principles established by the National Institutes of Health Guide for the Care and Use of Laboratory Animals (NIH Publications No. 8523, revised 2011) were followed. The research protocol was approved by the University of Wisconsin-Madison Institutional Animal Care and Use Committee (Protocol No. M005669).
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Foote, A.G., Lungova, V. & Thibeault, S.L. Piezo1-expressing vocal fold epithelia modulate remodeling via effects on self-renewal and cytokeratin differentiation. Cell. Mol. Life Sci. 79, 591 (2022). https://doi.org/10.1007/s00018-022-04622-6
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DOI: https://doi.org/10.1007/s00018-022-04622-6