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3D microenvironment attenuates simulated microgravity-mediated changes in T cell transcriptome

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Abstract

Human space travel and exploration are of interest to both the industrial and scientific community. However, there are many adverse effects of spaceflight on human physiology. In particular, there is a lack of understanding of the extent to which microgravity affects the immune system. T cells, key players of the adaptive immune system and long-term immunity, are present not only in blood circulation but also reside within the tissue. As of yet, studies investigating the effects of microgravity on T cells are limited to peripheral blood or traditional 2D cell culture that recapitulates circulating blood. To better mimic interstitial tissue, 3D cell culture has been well established for physiologically and pathologically relevant models. In this work, we utilize 2D cell culture and 3D collagen matrices to gain an understanding of how simulated microgravity, using a random positioning machine, affects both circulating and tissue-resident T cells. T cells were studied in both resting and activated stages. We found that 3D cell culture attenuates the effects of simulated microgravity on the T cells transcriptome and nuclear irregularities compared to 2D cell culture. Interestingly, simulated microgravity appears to have less effect on activated T cells compared to those in the resting stage. Overall, our work provides novel insights into the effects of simulated microgravity on circulating and tissue-resident T cells which could provide benefits for the health of space travellers.

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Data availability

The datasets generated and analyzed for this study will be available upon request.

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Acknowledgements

The authors would like to acknowledge support from NYUAD core technology platform (cell and molecular biology, optical imaging, and bioinformatics, and sequencing).

Funding

The authors acknowledge the support from New York University Abu Dhabi (NYUAD) Faculty Research Fund (AD266) and NYUAD Research Enhancement Fund (RE267).

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Authors and Affiliations

  1. Laboratory for Immuno Bioengineering Research and Applications, Division of Engineering, New York University Abu Dhabi, PO Box 129188, Abu Dhabi, United Arab Emirates

    Mei ElGindi, Jiranuwat Sapudom, Anna Garcia-Sabaté & Jeremy Teo

  2. Laboratory of Applied Nonlinear Dynamics, Division of Engineering, New York University Abu Dhabi, PO Box 129188, Abu Dhabi, United Arab Emirates

    Praveen Laws & Mohammed F. Daqaq

  3. Department of Mechanical and Biomedical Engineering, New York University, Brooklyn, NY, 11201, USA

    Jeremy Teo

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  1. Mei ElGindi
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Contributions

Conceptualization, ME, JS, AG-S, and JCT; formal analysis, ME, JS, PL; funding acquisition, JCT; investigation, ME and JS; methodology, ME and JS; supervision, JS, MD and JCT; visualization, ME, JS, and AG-S; writing—original draft, ME; writing—review and editing, JS, AG-S, and JCT. All authors have read and agreed to the published version of the manuscript

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Correspondence to Jeremy Teo.

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ElGindi, M., Sapudom, J., Laws, P. et al. 3D microenvironment attenuates simulated microgravity-mediated changes in T cell transcriptome. Cell. Mol. Life Sci. 79, 508 (2022). https://doi.org/10.1007/s00018-022-04531-8

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  • DOI: https://doi.org/10.1007/s00018-022-04531-8

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