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Characterization of the microRNA transcriptomes and proteomics of cochlear tissue-derived small extracellular vesicles from mice of different ages after birth

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Abstract

The cochlea is an important sensory organ for both balance and sound perception, and the formation of the cochlea is a complex developmental process. The development of the mouse cochlea begins on embryonic day (E)9 and continues until postnatal day (P)21 when the hearing system is considered mature. Small extracellular vesicles (sEVs), with a diameter ranging from 30 to 200 nm, have been considered a significant medium for information communication in both physiological and pathological processes. However, there are no studies exploring the role of sEVs in the development of the cochlea. Here, we isolated tissue-derived sEVs from the cochleae of FVB mice at P3, P7, P14, and P21 by ultracentrifugation. These sEVs were first characterized by transmission electron microscopy, nanoparticle tracking analysis, and western blotting. Next, we used small RNA-seq and mass spectrometry to characterize the microRNA transcriptomes and proteomes of cochlear sEVs from mice at different ages. Many microRNAs and proteins were discovered to be related to inner ear development, anatomical structure development, and auditory nervous system development. These results all suggest that sEVs exist in the cochlea and are likely to be essential for the normal development of the auditory system. Our findings provide many sEV microRNA and protein targets for future studies of the roles of cochlear sEVs.

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All the data and material analyzed in this research are included in this article and its supplementary files.

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Acknowledgements

Thanks to all colleagues who contributed to this research.

Funding

This work was supported by grants from the National Key RD Program of China (nos. 2021YFA1101300, 2020YFA0112503), the Strategic Priority Research Program of the Chinese Academy of Sciences (No. XDA16010303), the National Natural Science Foundation of China (nos. 82171149, 81970892, 82030029, 81970882), the Natural Science Foundation of Jiangsu Province (nos. BK20190062 and  BE2019711), the Science and Technology Department of Sichuan Province (no. 2021YFS0371), the Shenzhen Fundamental Research Program (no. JCYJ20190814093401920, JCYJ20210324125608022), Open Research Fund of State Key Laboratory of Genetic Engineering, Fudan University (No. SKLGE-2109), and the Fundamental Research Funds for the Central Universities for the Support Program of Zhishan Youth Scholars of Southeast University (no. 2242021R41136).

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  1. Pei Jiang, Xiangyu Ma, Shanying Han and Leyao Ma contributed equally to this work.

Authors and Affiliations

  1. State Key Laboratory of Bioelectronics, Department of Otolaryngology Head and Neck Surgery, Zhongda Hospital, School of Life Sciences and Technology, Jiangsu Province High-Tech Key Laboratory for Bio-Medical Research, Southeast University, Nanjing, 210096, China

    Pei Jiang, Xiangyu Ma, Jingru Ai, Leilei Wu, Yuan Zhang, Hairong Xiao, Mengyao Tian, Shasha Zhang & Renjie Chai

  2. State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing, 210096, China

    Shanying Han & Leyao Ma

  3. Department of Chemistry, Department of Biochemistry, Purdue University, West Lafayette, Indiana, 47907, USA

    W. Andy Tao

  4. Center for Cancer Research, Purdue University, West Lafayette, Indiana, 47907, USA

    W. Andy Tao

  5. Co-Innovation Center of Neuroregeneration, Nantong University, Nantong, 226001, China

    Renjie Chai

  6. Department of Otolaryngology Head and Neck Surgery, Sichuan Provincial People’s Hospital, University of Electronic Science and Technology of China, Chengdu, China

    Renjie Chai

  7. Institute for Stem Cell and Regeneration, Chinese Academy of Science, Beijing, China

    Renjie Chai

  8. Beijing Key Laboratory of Neural Regeneration and Repair, Capital Medical University, Beijing, 100069, China

    Renjie Chai

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Jiang, P., Ma, X., Han, S. et al. Characterization of the microRNA transcriptomes and proteomics of cochlear tissue-derived small extracellular vesicles from mice of different ages after birth. Cell. Mol. Life Sci. 79, 154 (2022). https://doi.org/10.1007/s00018-022-04164-x

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