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The deubiquitinase OTUD5 regulates Ku80 stability and non-homologous end joining

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Abstract

The ability of cells to repair DNA double-strand breaks (DSBs) is important for maintaining genome stability and eliminating oncogenic DNA lesions. Two distinct and complementary pathways, non-homologous end joining (NHEJ) and homologous recombination (HR), are employed by mammalian cells to repair DNA DSBs. Each pathway is tightly controlled in response to increased DSBs. The Ku heterodimer has been shown to play a regulatory role in NHEJ repair. Ku80 ubiquitination contributes to the selection of a DSB repair pathway by causing the removal of Ku heterodimers from DSB sites. However, whether Ku80 deubiquitination also plays a role in regulating DSB repair is unknown. To address this question, we performed a comprehensive study of the deubiquitinase specific for Ku80, and our study showed that the deubiquitinase OTUD5 serves as an important regulator of NHEJ repair by increasing the stability of Ku80. Further studies revealed that OTUD5 depletion impaired NHEJ repair, and hence reduced overall DSB repair. Furthermore, OTUD5-depleted cells displayed excess end resection; as a result, HR repair was facilitated by OTUD5 depletion during the S/G2 phase. In summary, our study demonstrates that OTUD5 is a specific deubiquitinase for Ku80 and establishes OTUD5 as an important and positive regulator of NHEJ repair.

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Acknowledgements

We are grateful to Dr. Jiadong Wang for his suggestion and help. We thank Qihua He for her suggestion and help, and Center of Medical and Health Analysis, Peking University, for confocal microscopy. We appreciate the ALENABIO (Xi’an, China) Company (http://www.alenabio.com) for the pathological micro-tissues (Cat. No. BC03119a). We deeply appreciate help from Ning Kon for editing our manuscript.

Funding

Wenhui Zhao was supported by the National Natural Science Foundation of China (Grant No. 85141044).

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Authors and Affiliations

  1. Department of Biochemistry and Molecular Biology, Peking University Health Science Center, Beijing Key Laboratory of Protein Posttranslational Modifications and Cell Function, 38 Xueyuan Road, Beijing, 100191, China

    Fangzhou Li, Qianqian Sun, Kun Liu, Haichao Han, Ning Lin, Zebin Mao, Tanjun Tong & Wenhui Zhao

  2. Department of Laboratory Animal Science, Peking University Health Science Center, 38 Xueyuan Road, Beijing, 100191, China

    Feng Tian

  3. Jingjie PTM BioLab, Co. Ltd, Hangzhou Economic and Technological Development Area, Hangzhou, 310018, China

    Zhongyi Cheng

  4. Rheumatic Immunology Department, Peking University Shenzhen Hospital, Shenzhen, 518035, China

    Yueming Cai

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  1. Fangzhou Li
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Contributions

FL and WZ, conceived and designed the study that led to the submission, acquired data, and interpreted the results; FL performed the experiment; QS, KL, HH, QH, ZC, YM, and FT participated in the revision of the manuscript; ZM and TT approved the final version; and WZ was the corresponding author. The authors declare that they have no conflicts of interest associated with the contents of this article.

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Correspondence to Wenhui Zhao.

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Li, F., Sun, Q., Liu, K. et al. The deubiquitinase OTUD5 regulates Ku80 stability and non-homologous end joining. Cell. Mol. Life Sci. 76, 3861–3873 (2019). https://doi.org/10.1007/s00018-019-03094-5

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