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Myelin-associated proteins block the migration of olfactory ensheathing cells: an in vitro study using single-cell tracking and traction force microscopy

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Abstract

Newly generated olfactory receptor axons grow from the peripheral to the central nervous system aided by olfactory ensheathing cells (OECs). Thus, OEC transplantation has emerged as a promising therapy for spinal cord injuries and for other neural diseases. However, these cells do not present a uniform population, but instead a functionally heterogeneous population that exhibits a variety of responses including adhesion, repulsion, and crossover during cell–cell and cell–matrix interactions. Some studies report that the migratory properties of OECs are compromised by inhibitory molecules and potentiated by chemical gradients. Here, we demonstrated that rodent OECs express all the components of the Nogo receptor complex and that their migration is blocked by myelin. Next, we used cell tracking and traction force microscopy to analyze OEC migration and its mechanical properties over myelin. Our data relate the decrease of traction force of OEC with lower migratory capacity over myelin, which correlates with changes in the F-actin cytoskeleton and focal adhesion distribution. Lastly, OEC traction force and migratory capacity is enhanced after cell incubation with the Nogo receptor inhibitor NEP1-40.

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Acknowledgments

The authors thank R. Rycroft for linguistic advice and G. Tormen for technical assistance. This work was supported by FP7-PRIORITY, the MICINN (BFU2009-10848) and grants from the Instituto Carlos III (PI11/03028) and SGR2009-366 (Generalitat of Catalunya) to JADR. SN and PC were supported by the MICINN. DR is supported by a fellowship from the National Commission for Science and Technology (CONICYT, Chile). AB is a Sara Borrell postdoctoral research of the Instituto Carlos III. XT acknowledges support of the Spanish Ministry for Science and Innovation (BFU2009-07595) and the European Research Council (Grant Agreement 242993). F. Wandosell was supported by MICINN (SAF2009-12249-C02-01).

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Authors and Affiliations

  1. Molecular and Cellular Neurobiotechnology, Institute for Bioengineering of Catalonia (IBEC), Barcelona Science Park, University of Barcelona, Barcelona, Spain

    Sara Nocentini, Diego Reginensi, Patricia Carulla, Ana Bribian & José A. del Río

  2. Department of Cell Biology, Faculty of Biology, University of Barcelona, Barcelona, Spain

    Sara Nocentini, Diego Reginensi, Patricia Carulla, Ana Bribian & José A. del Río

  3. Centro de Investigación Biomédica en Red de Enfermedades Neurodegenerativas (CIBERNED), Barcelona, Spain

    Sara Nocentini, Diego Reginensi, Patricia Carulla, Ana Bribian & José A. del Río

  4. Integrative Cell and Tissue Dynamics, Institute for Bioengineering of Catalonia (IBEC), Barcelona Science Park, University of Barcelona, Barcelona, Spain

    Simón Garcia & Xavier Trepat

  5. Institució Catalana de Recerca i Estudis Avançats (ICREA), Barcelona, Spain

    Xavier Trepat

  6. Facultat de Medicina, Universitat de Barcelona, Barcelona, Spain

    Xavier Trepat

  7. Centro de Investigación Biomédica en Red de Enfermedades Respiratorias (CIBERES), Barcelona, Spain

    Xavier Trepat

  8. Centro de Biología Molecular “Severo Ochoa”, Nicolás Cabrera, 1, Universidad Autónoma de Madrid (CBM-UAM), Madrid, Spain

    María Teresa Moreno-Flores & Francisco Wandosell

  9. Centro de Investigación Biomédica en Red de Enfermedades Neurodegenerativas (CIBERNED), CBM-UAM, Madrid, Spain

    Francisco Wandosell

Authors
  1. Sara Nocentini
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  2. Diego Reginensi
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  3. Simón Garcia
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  4. Patricia Carulla
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  5. María Teresa Moreno-Flores
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  6. Francisco Wandosell
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  7. Xavier Trepat
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  8. Ana Bribian
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  9. José A. del Río
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Corresponding authors

Correspondence to Ana Bribian or José A. del Río.

Additional information

S. Nocentini and D. Reginensi contribute equally to this study.

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18_2011_893_MOESM1_ESM.tif

Fig. S1. Myelin treatment activates RhoA and induces ERK1-2 phosphorylation in TEG3 cells. Time course of RhoA activation (a) and ERK1-2 phosphorylation (b) in TEG3 cells after myelin treatment. See the sections Materials and methods and Results for details (TIFF 2036 kb)

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Nocentini, S., Reginensi, D., Garcia, S. et al. Myelin-associated proteins block the migration of olfactory ensheathing cells: an in vitro study using single-cell tracking and traction force microscopy. Cell. Mol. Life Sci. 69, 1689–1703 (2012). https://doi.org/10.1007/s00018-011-0893-1

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  • DOI: https://doi.org/10.1007/s00018-011-0893-1

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