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Interaction between STAT3 gene polymorphisms and smoking on Crohn’s disease susceptibility: a case–control study in a Chinese Han population

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Abstract

Aims

The aim of this study was to investigate the impact of the signal transducer and activator of transcription 3 (STAT3) gene and additional STAT3 gene–smoking interaction on Crohn’s disease (CD) risk based on a Chinese population.

Methods

A total of 1012 participants (491 men, 521 women), were selected, including 502 CD patients and 510 normal controls. The mean age of all participants was 42.3 ± 11.2 years. Logistic regression model was used to examine the association between single nucleotide polymorphism (SNP) of STAT3 and CD risk; the odds ratio (OR) and 95 % confident interval (95 % CI) were calculated. Generalized multifactor dimensionality reduction was employed to analyze the interaction among several SNPs.

Results

Logistic analysis showed the significant association between genotypes of variants in two SNP and decreased CD risk, after covariates adjustment. The carriers of homozygous mutant of two SNP polymorphism revealed decreased CD risk than those with wild-type homozygotes; OR (95 % CI) was 0.75 (0.59–0.93) and 0.68 (0.57–0.91), respectively. There was a significant two-locus model (p = 0.0107) involving rs744166 and smoking, indicating a potential gene–environment interaction between rs744166 and smoking. Overall, the cross-validation consistency was 10/10, and the testing accuracy was 62.17 %, and never smokers with TC or CC genotype have the lowest CD risk, compared to smokers with TT genotype; OR (95 % CI) was 0.52 (0.31–0.82), after covariate adjustment.

Conclusions

Our results support an important association of rs744166 and rs4796793 with decreased CD risk, and additional interaction between rs744166 and smoking.

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Acknowledgments

The writing of this paper was supported by Changzhou Center for Disease Control and Prevention and the First Hospital of Hengyang. We thank all the partners and staff who helped us in the process of this study.

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    Authors and Affiliations

    1. Inspection Center, Changzhou Center for Disease Control and Prevention, No. 203, Taishan Road, The New North District, Changzhou, 213022, Jiangsu, People’s Republic of China

      Jun-hong Li, Hong-bing Tang, Dong-ping Bao & Jun-min Ji

    2. Department of Clinical Laboratory, The First Hospital of Hengyang, Hengyang, 421001, Hunan, People’s Republic of China

      Ning Wu

    3. Department of Blood Quality Management, Changzhou Blood Center, Changzhou, 213004, Jiangsu, People’s Republic of China

      Hong-mei Yang

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    1. Jun-hong Li
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    Correspondence to Jun-min Ji.

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    Responsible Editor: John Di Battista.

    J.-h. Li and N. Wu have contributed equally to this work.

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    Li, Jh., Wu, N., Yang, Hm. et al. Interaction between STAT3 gene polymorphisms and smoking on Crohn’s disease susceptibility: a case–control study in a Chinese Han population. Inflamm. Res. 65, 573–578 (2016). https://doi.org/10.1007/s00011-016-0941-2

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    • DOI: https://doi.org/10.1007/s00011-016-0941-2

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