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Transcription-related element gene expression pattern differs between microglia and macrophages during inflammation

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Abstract

Objective and design

Microglia and macrophages play an important role in the innate and adaptive immune systems. Although the resident location of these cells is different, their functions during the polarization response due to various stimuli are very similar. The present study aimed to analyze differences in microglial and macrophage gene expression during inflammation.

Methods

Mouse microglial BV-2 cells were exposed to LPS (10 ng/ml). The levels of gene expression were measured using real-time RT-PCR and whole transcriptome shotgun sequencing.

Results

The level of Jmjd3 gene expression in activated microglia showed a similar pattern to that of macrophages. In both cell types, genes associated with the inflammation response were generally increased whereas genes associated with metabolic and biosynthetic processes were decreased. However, the expression of transcription-related elements other than genes encoding histone modification enzymes showed a significantly different pattern between microglia and macrophages.

Conclusion

Although the function and the gene expression levels of histone modification enzymes showed a similar pattern in microglia and macrophages during inflammation, the expression of transcription-related elements in both cell types showed a completely different pattern.

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Acknowledgments

We are grateful to Hee-Sun Kim for providing BV-2 cells. This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIP) (No. 2011-0030049).

Conflict of interest

The author(s) declare that they have no competing interests.

Author information

Authors and Affiliations

  1. Department of Molecular and Life Sciences, Hanyang University, Ansan, 426-791, Republic of Korea

    Hyung Tae Lee, Se Kye Kim, Sun Hwa Kim & Young Gyu Chai

  2. Korean Bioinformation Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, 305-806, Republic of Korea

    Kyung Kim & Namshin Kim

  3. Department of Biomedical Informatics, Ajou University School of Medicine, Suwon, 443-749, Republic of Korea

    Kyung Kim

  4. Division of Integrative Biosciences and Biotechnology, POSTECH, Pohang, Gyeongbuk, 790-784, Republic of Korea

    Chae Hyun Lim & Tae-Young Roh

  5. Department of Life Science and Division of Molecular and Life Sciences, POSTECH, Pohang, Gyeongbuk, 790-784, Republic of Korea

    Jihwan Park & Tae-Young Roh

  6. Department of Bionanotechnology, Hanyang University, Seoul, 133-791, Republic of Korea

    Young Gyu Chai

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  1. Hyung Tae Lee
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Correspondence to Young Gyu Chai.

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Lee, H.T., Kim, S.K., Kim, S.H. et al. Transcription-related element gene expression pattern differs between microglia and macrophages during inflammation. Inflamm. Res. 63, 389–397 (2014). https://doi.org/10.1007/s00011-014-0711-y

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  • DOI: https://doi.org/10.1007/s00011-014-0711-y

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