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Mast cells cultured from IL-3-treated mice show impaired responses to bacterial antigen stimulation

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Abstract

Objective and design

This study exploits the biological activity of interleukin (IL)-3 to generate high yields of peritoneal mast cells ex vivo in order to examine pro-inflammatory immune responses in ex-vivo culture.

Material or subjects

Mast cells were obtained from the peritoneal cavity of C57BL/6 mice.

Treatment

Mice were injected intraperitoneally twice per day for 5 days with IL-3 (40–50 μg/ml) to increase mast cell numbers.

Methods

Histological studies examined mast cell numbers in the peritoneal cavity, intestine, lung, spleen and skeletal muscle. Peritoneal mast cells cultured ex vivo (PCMCs) were stimulated for 24 h with lipopolysaccharide and Bordetella pertussis antigen and secretion of tumour necrosis factor-α, IL-6, IL-4, IL-5, IL-10 and interferon-γ into supernatant was measured by commercial ELISA. Cell surface marker expression of FcεRI, c-kit, OX40L and TLR2 was measured by flow cytometry. Mast cell degranulation was measured using a β-hexosaminidase assay.

Results

IL-3 treatment increases mast cell numbers in the peritoneal cavity, spleen and muscle but not intestine and lung of C57BL/6 mice. PCMCs generated from IL-3-treated mice exhibit impaired growth, differentiation and responses to activation as measured by decreased cytokine secretion and cell surface marker expression.

Conclusion

Mast cells cultured from IL-3-treated mice show impaired responses.

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Acknowledgments

We would like to thank Eric Callaghan for providing facilities for microscopic work at School of Biology and Environmental Science, University College Dublin, Ireland. We also would like to thank the staff of the Bioresource Unit, at Dublin City University, Ireland, and Sina Heydrich at Charité, Germany, for excellent technical assistance. This project was supported in part by the Ph.D. research and training programme in Target-driven Therapeutic and Theranostics. This programme is being financed by the Higher Education Authority as part of a National Graduate Training Programme in Biopharmaceuticals and Pharmacological Sciences in partnership with University College Dublin, University College Cork, Trinity College Dublin and Queens University Belfast. We also would like to acknowledge support by the COST Action BM1007 “Mast cells and basophils—Targets for innovative therapies”. Tamás Visnovitz was supported by the Irish Research Council for Science, Engineering and Technology (IRCSET).

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Authors and Affiliations

  1. Parasite Immune Modulation Group, School of Nursing and Human Sciences, Faculty of Science and Health, Dublin City University, Glasnevin, Dublin 9, Ireland

    Krisztina V. Vukman, Paul N. Adams & Sandra M. O’Neill

  2. School of Biology and Environmental Science, Science Centre West, University College Dublin, Belfield, Dublin 4, Ireland

    Tamás Visnovitz

  3. Department of Dermatology and Allergy, Allergie-Centrum-Charité/ECARF, Charité-Universitätsmedizin, Berlin, Germany

    Martin Metz & Marcus Maurer

Authors
  1. Krisztina V. Vukman
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  2. Tamás Visnovitz
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  3. Paul N. Adams
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  4. Martin Metz
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  5. Marcus Maurer
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  6. Sandra M. O’Neill
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Corresponding author

Correspondence to Sandra M. O’Neill.

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Responsible Editor: Andras Falus.

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Vukman, K.V., Visnovitz, T., Adams, P.N. et al. Mast cells cultured from IL-3-treated mice show impaired responses to bacterial antigen stimulation. Inflamm. Res. 61, 79–85 (2012). https://doi.org/10.1007/s00011-011-0394-6

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  • DOI: https://doi.org/10.1007/s00011-011-0394-6

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