Abstract
Objective
To evaluate the regulatory effects of honokiol on high-glucose (HG)-induced inflammatory responses of human renal mesangial cells (HRMCs).
Materials and methods
We performed MTS assays to determine the non-cytotoxic concentration of honokiol for HRMCs. Enzyme-linked immunosorbent assays were performed to analyze the expressions of the proteins interleukin (IL)-1β, IL-18, tumor necrosis factor (TNF)-α, transforming growth factor (TGF)-β1, monocyte chemoattractant protein (MCP)-1, macrophage inflammatory protein (MIP)-1α, RANTES, and prostaglandin (PG) E2. The total nitric oxide (NO) concentration was determined using the Griess reaction.
Results
Treatment with 50 mmol/L glucose markedly increased the level of IL-1β, IL-18, TNF-α, PGE2, NO, TGF-β1, MCP-1, MIP-1α, and RANTES. Honokiol (~20 μmol/L) treatment inhibited the HG-induced expression of inflammatory cytokines such as IL-1β, IL-18, TNF-α, PGE2, NO, and TGF-β1 in a dose-dependent manner. Moreover, it markedly inhibited the expression of chemokines such as MCP-1, MIP-1α, and RANTES, which are upregulated under HG conditions.
Conclusion
Honokiol inhibits the HG-induced expression of inflammatory factors in HRMCs. Honokiol may be considered a promising drug with potent anti-inflammatory activities in addition to its strong anti-cancer, anti-angiogenesis, and anti-neurodegenerative effects.
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Acknowledgments
This work was supported by grants from Science and Technology Foundation (2009C33150, 20090833B24) and Education Foundation (20070114) of Zhejiang Provincial, China. This manuscript is proofread by a native English professional with science background at Elixigen Corporation.
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Responsible Editor: Ian Ahnfelt-Rønne.
J.-P. Wu and W. Zhang contributed equally to this work.
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Wu, JP., Zhang, W., Wu, F. et al. Honokiol: an effective inhibitor of high-glucose-induced upregulation of inflammatory cytokine production in human renal mesangial cells. Inflamm. Res. 59, 1073–1079 (2010). https://doi.org/10.1007/s00011-010-0227-z
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DOI: https://doi.org/10.1007/s00011-010-0227-z