Abstract
The aim of this study was to evaluate the feasibility of a chromatographic method to easily detect circulating anti-thyroid hormone autoantibodies (THBA), to calculate their affinity constant and the total thyroid hormone (TH) levels in presence of THBA. This method was applied to sera from 4 subject with suspected THBA and 20 controls (10 normal subjects and 10 patients with thyroid dysfunctions). After a short incubation with 125I-T3 or T4 tracer solution containing 8-anilino-1-naphthalenesulfonic acid as inhibitor of the binding of TH to plasma proteins without affecting THBA binding, the samples were chromatographied on prepacked Sephadex LH 20 columns and eluted with TRIS buffer to separate free TH from THBA-bound TH. Samples were considered THBA positive when radioactivity values in TRIS eluates were higher than in controls. THBA-bound TH were subsequently eluted with methanol and used to calculate the total TH present in patients with THBA. After a validation test using two standardized methods, we propose this method to quickly detect THBA in samples with suspected spuriously high values of free and total TH.
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References
Robbins J., Rall J.E, Rawson R.W. An unusual instance of thyroxine binding by human serum gammaglobulin. J. Clin. Endocrinol. Metab. 16: 573, 1956.
Benvenga S., Trimarchi F., Robbins J. Circulating thyroid hormone autoantibodies. J. Endocrinol. Invest. 10: 605, 1987.
Sakata S., Nakamura S., Miura K. Autoantibodies against thyroid hormones or iodothyronine. Implications in diagnosis, thyroid function, treatment and pathogenesis. Ann. Int. Med. 103: 579, 1985.
Beck-Peccoz P., Medri G., Rossi C.M., Romelli P.B. Gli anticorpi circolanti antiiodotironine come fonti di errori nella valutazione immunometrica degli ormoni tiroidei. Ligand Quartely 5: 427, 1986.
Beck-Peccoz P., Medri G., Rossi C.M., Faglia G. Anti-thyroid hormone autoantibodies. Progr. Med. Lab. 2: 57, 1988.
Wu S.E., Green W.L. Triiodothyronine (T3)-binding immunoglobulins in a euthyroid woman: Effects on measurement of T3 (RIA) and on T3 turnover. J. Clin. Endocrinol. Metab. 42: 642, 1976.
Blanc M.H., Despont J.P., Burger A.G. Thyroid hormone-binding antibodies (Letter). N. Engl. J. Med. 297: 1068, 1977.
Ginsberg J., Segal D., Herlich R.M., Walfish P.G. Inappropriate triiodothyronine (T3) and thyroxine (T4) radioimmunoassay levels secondary to circulating thyroid hormone autoantibodies. Clin. Endocrinol. (Oxf.) 8: 133, 1978.
Chopra I.J., Van Herle A.J., Teco G.N.C., Nguyen A.H. Serum-free thyroxine in thyroidal and nonthyroidal illnesses: a comparison of measurements by radioimmunoassay, equilibrium dialysis and free thyroxine index. J. Clin. Endocrinol. Metab. 51: 135, 1980.
De Baets M., Sels J., van Breda Vriesman P.B., Elewaut A., Vermuelen A., Willems P., Coenegracth J Monoclonal triiodothyronine (T3) immunoglobulins in a euthyroid woman. Clin. Chim. Acta 118: 293, 1982.
Moroz L.A., Meltzer S.J., Blastonsky C.H. Thyroid disease with monoclonal (IgG lambda) antibodies to triiodothyronine and tetraiodothyronine. J. Clin. Endocrinol. Metab. 56: 1009, 1983.
Nakamura S., Sugimoto M., Kosaka J., Komaki T., Kamura H., Miyazaki T., Matsumoto H., and Sakata S Immunogenetic study of thyroid hormones autoantibodies in a family. J. Endocrinol. Invest. 14: 131, 1991.
Crinò A., Borrelli P., Salvadori R., Cortelazzi D., Roncoroni R., Beck-Peccoz P. Anti-iodothyronine autoantibodies in a girl with hyperthyroidism due to pituitary resistance to thyroid hormones. J. Endocrinol. Invest. 15: 113, 1992.
Quiping Q., Yintang B., Meijuan W., Yiming G., Hua S. Radioimmunoassay for autoantibodies to triiodothyronine and its preliminary clinical application. Biothec RIA 93. The impact of biotecnology on autoimmunity Florence, Italy June 2–4, 1993 (Abstr).
Sarui H., Nagai K., Kametani M., Ogawa T., Tokimitsu N., Sakata S. Autoimmune hepatitis and hypothyroidism associated to antithyroid hormone autoantibodies. Intern. Med. 32: 21, 1993.
Romelli P.B., Pennisi F., Vancheri L. Measurement of free thyroid hormones in serum by column adsorbtion chromatography and radioimmunoassay. J. Endocrinol. Invest. 12: 26, 1979.
Beck-Peccoz P., Romelli P.B., Cattaneo M.G., Faglia G. Free T4 and Free T3 measurement in patients with antiiodothyronine autoantibodies. In: Albertini A., Ekins R.P. (Eds.), Free Hormones in Blood. Elsevier Biomedical Press, Amsterdam, 1982, p. 231.
Trimarchi F., Benvenga S., Costante G., Barbera C., Melluso R., Marcocci C., Chiovato L., De Luca F., Consolo F. Identification and characterization of circulating thyroid hormone autoantibodies in thyroid diseases, in autoimmune non-thyroid illness and in lymphoreticular system diorders. J. Endocrinol. Invest. 6: 203, 1983.
Benvenga S., Cahnmann H.J., Robbins J. Human apolipoproteins A1 and C are novel thyroxine binding protein. In: Nagataki S., Torizuka K. (Eds.), The Thyroid Gland. Elsevier Press, Amsterdam, 1988, p. 343.
Robbins J., Cheng S.Y., Gershengorn M.C., Glinoer D., Cahnmann H.J., Edelnoch H. Thyroxine transport proteins of plasma. Molecular properties and biosynthesis. Recent Prog. Horm. Res. 34: 477, 1978.
Robbins J. and Johnson M.L. Theoretical consideration in the transport of the thyroid hormones in the blood. In: Ekins R., Faglia G., Pennisi F., Pinchera A. (Eds.), Free thyroid hormones. Excerpta Medica, Amsterdam, 1979, p. 1.
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Savastano, S., Tommaselli, A.P., Valentino, R. et al. A quick method to detect circulating anti-thyroid hormone autoantibodies. J Endocrinol Invest 18, 9–16 (1995). https://doi.org/10.1007/BF03349690
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DOI: https://doi.org/10.1007/BF03349690