Abstract
The aim of this study was to determine the protein pattern of human thyroid fine needle aspiration fluid (FNA) using a proteomic approach. FNA proteins were separated using 2-dimensional gel electrophoresis (2DE), digested and then analyzed by peptide mass fingerprinting. For the first time, we provided an image of the protein components of the FNA, in which approximately 220 protein spots can be identified. The proteome analysis revealed a specific fingerprint of FNA with proteins appertaining to various functional systems. Our preliminary results of FNA protein pattern could be a starting point in studying the presence of potential markers implicated in thyroid diseases.
Similar content being viewed by others
References
Hu S, Loo JA, Wong DT. Human body fluid proteome analysis. Proteomics 2006, 6: 6326–53.
Celis JE, Gromov P, Cabezon T, et al. Proteomic characterization of the interstitial fluid perfusing the breast tumor microenvironment: a novel resource for biomarker and therapeutic target discovery. Mol Cell Proteomics 2004, 3: 327–44.
Alexander H, Stegner AL, Wagner-Mann C, Du Bois GC, Alexander S, Sauter ER. Proteomic analysis to identify breast cancer biomarkers in nipple aspirate fluid. Clin Cancer Res 2004, 10: 7500–10.
Vascotto C, Salzano AM, D’Ambrosio C, et al. Oxidized transthyretin in amniotic fluid as an early marker of preeclampsia. J Proteome Res 2007, 6: 160–70.
Krause K, Schierhorn A, Sinz A, et al. Toward the application of proteomics to human thyroid tissue. Thyroid 2006, 16: 1131–43.
Giusti L, Baldini C, Bazzichi L, et al. Proteome analysis of whole saliva: a new tool for rheumatic diseases — the example of Sjögren’s syndrome. Proteomics 2007, 7: 1634–43.
Hochstrasser DF, Patchornik A, Merril CR. Development of polyacrylamide gels that improve the separation of proteins and their detection by silver staining. Anal Biochem 1988, 173: 412–23.
Candiano G, Bruschi M, Musante L, et al. Blue silver: a very sensitive colloidal Coomassie G-250 staining for proteome analysis. Electrophoresis 2004, 25: 1327–33.
Torres-Cabala C, Bibbo M, Panizo-Santos A, et al. Proteomic identification of new biomarkers and application in thyroid cytology. Acta Cytol 2006, 50: 518–28.
Krause K, Karger S, Schierhorn A, Poncin S, Many MC, Fuhrer D. Proteomic profiling of cold thyroid nodules. Endocrinology 2007, 148: 1754–63.
Srisomsap C, Subhasitanont P, Otto A, et al. Detection of cathepsin B up-regulation in neoplastic thyroid tissues by proteomic analysis. Proteomics 2002, 2: 706–12.
Brown LM, Helmke SM, Hunsucker SW, et al. Quantitative and qualitative differences in protein expression between papillary thyroid carcinoma and normal thyroid tissue. Mol Carcinog 2006, 45: 613–26.
Paron I, D’Ambrosio C, Scaloni A, et al. A differential proteomic approach to identify proteins associated with thyroid cell transformation. J Mol Endocrinol 2005, 34: 199–207.
Ehmann M, Felix K, Hartmann D, et al. Identification of potential markers for the detection of pancreatic cancer through comparative serum protein expression profiling. Pancreas 2007, 34: 205–14.
Zhang Z, Bast RC, Yu Y, et al. Three biomarkers identified from serum proteomic analysis for the detection of early stage ovarian cancer. Cancer Res 2004, 64: 5882–90.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Giusti, L., Iacconi, P., Ciregia, F. et al. Proteomic analysis of human thyroid fine needle aspiration fluid. J Endocrinol Invest 30, 865–869 (2007). https://doi.org/10.1007/BF03349229
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF03349229