Skip to main content
SpringerLink
Log in

Renal β-adrenoceptors in thyroxine-treated rats

  • Published:
Journal of Endocrinological Investigation Aims and scope Submit manuscript

Abstract

The number of β-adrenoceptors was measured in cardiac and renal cortex membranes from controls and thyroxine-treated rats. Two methods of membrane preparation (sucrose- and KCI-extraction) were compared. KCI-extraction yielded a higher number of β-adrenoceptors, accompanied by increased activity of the plasma membrane enzyme marker 5′-nucleotidase. Thyroxine increased the number of β-adrenoceptors in both cardiac and renal cortex membranes prepared with both methods. These results suggest that: i) Enrichment of the membrane fraction may explain the higher number of β-adrenoceptors found with KCI, ii) Thyroid hormone plays a role in the regulation of β-adrenoceptors in the renal cortex.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Stiles G.L,. Caron M.G., Lefkowitz R.J. The β-adrenergic receptor: Biochemical mechanisms of physiological regulation. Physiol. Rev. 64: 64, 1984.

    Google Scholar 

  2. Krawietz W., Wierdan K., Erdmann E. Effect of thyroid status on β-adrenergic receptor, adenylatecyclase activity and guanine nucleotide regulatory unit in rat cardiac and erythrocyte membranes. Biochem. Pharmacol. 37: 2463, 1982.

    Article  Google Scholar 

  3. Churchill P.C., Churchill M.C., McDonald F.D. Evidence that β-adrenoceptor activation mediates isoproterenol-stimulated renin secretion in the rat. Endocrinology 113: 687, 1983.

    Article  CAS  PubMed  Google Scholar 

  4. Atlas S.A., Sealey J.E., Larag J.H., Moon C. Plasma renin and “prorenin” in essential hypertension during sodium depletion, beta-blockade and reduced arterial pressure. Lancet 2: 785,1977.

    Article  CAS  PubMed  Google Scholar 

  5. Snavely M.D., Motulsky H.J., Moustafa L.C., Insel P.A. β-adrenergic receptor subtypes in the rat renal cortex. Selective regulation of β1-adrenergic receptor number. Circ. Res. 51: 504, 1982.

    Article  CAS  PubMed  Google Scholar 

  6. Baker S.P., Potter L.T. Cardiac β-adrenoceptors during normal growth of male and female rats. Br. J. Pharmacol. 68: 65, 1980.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  7. Bristow M.R., Ginsburg R., Minobe W., Cubicciotti R.S., Sageman W.S., Lurie K, Billingham M.E., Harrison D.C., Stinson E.B. Decreased catecholamine sensitivity and β-adrenergic-receptor density in failing human hearts. N. Engl. J. Med. 307: 205, 1982.

    Article  CAS  PubMed  Google Scholar 

  8. Neville D.M.Jr. Isolation of an organ specific protein antigen from cell-surface membrane of rat liver. Biochem. Biophys. Acta 154: 540, 1968.

    CAS  PubMed  Google Scholar 

  9. Lowry O.H., Rosebrough N.J., Farr A.L., Randall R.J. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 793: 265, 1951.

    Google Scholar 

  10. Scatchard G. The attractions of proteins for small molecules and ions. Ann. NY Acad. Sci. 51: 660, 1949.

    Article  CAS  Google Scholar 

  11. Baker S.P., Potter L.T. Biochemical studies of cardiac β-adrenoceptors and their clinical significance. Circ. Res. (Suppl. 1)46: 1–38, 1980.

    Article  Google Scholar 

  12. Noguchi A. Normal ontogeny of α1-adrenergic receptor in rat liver is thyroid hormone dependent. Endocrinology 113: 672, 1983.

    Article  CAS  PubMed  Google Scholar 

  13. Bradley S.E., Stephan F., Coelho J.B., Reville P. The thyroid and the kidney. Kidney Int. 6: 346, 1974.

    Article  CAS  PubMed  Google Scholar 

  14. Holmes E.W., Discala V.A. Studies on the exaggerated natriuretic response to saline infusion in the hypothyroid rat. J. Clin. Invest. 49: 1224, 1970.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  15. Insel P.A., Snavely M.D. Catecholamines and the kidney: Receptors and renal function. Ann. Rev. Physiol. 43: 625, 1981.

    Article  CAS  Google Scholar 

  16. Hauger-Klevene J.H., Brown H., Zavaleta J. Plasma renin activity in hyper- and hypothyroidism. Effect of adrenergic blocking agents. J. Clin Endocrinol. Metab. 34: 625, 1972.

    Article  CAS  PubMed  Google Scholar 

  17. Bouhnik J., Galen F.X,. Clauser E., Menard J., Corvol P. The renin-angiotensin system in thyroidectomized rats. Endocrinology 108: 647, 1981.

    Article  CAS  PubMed  Google Scholar 

  18. Haro J.M., García-Torres L., Jodar E., Andrade J.. Baz M.J., Vargas F. XVII Congreso Nacional de Bioquimica. PL67. Oviedo (Spain). “Abstract”.

  19. Li-Fen Y., Baker S.P., Katovich M.J. Thyroxine, renal β-adrenergic receptors and dipso-genesis in food-deprived rats. Am. J. Physiol. 254: R33, 1988.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Departamento de Bioquímica, Facultad de Medicina, E 18012, Granada, Spain

    J. M. Haro, J. M. Sabio & F. Vargas

Authors
  1. J. M. Haro
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. J. M. Sabio
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. F. Vargas
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Haro, J.M., Sabio, J.M. & Vargas, F. Renal β-adrenoceptors in thyroxine-treated rats. J Endocrinol Invest 15, 605–608 (1992). https://doi.org/10.1007/BF03344933

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF03344933

Key-words

Search

Navigation