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The temporal relationships of synthesis and phosphorylation in stress proteins 70 and 90 in aged caloric restricted rats exposed to bleomycin

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Abstract

A single intraperitoneal injection of the human therapeutic drug bleomycin (BL) was administered to three groups of male Fischer 344 rats at time 0, and the incorporation of [35S]methionine (“synthesis”) and phosphorylation patterns of stress proteins (sps/hsps) from bone marrow cells were analyzed over time by two- dimensional electrophoresis and fluorography. Two groups of rats, young ad libitum (Y/AL — 3 months) and old ad libitum (O/AL — 28 months), had free access to rat chow, and a third group of old rats (O/CR — 28 months) were maintained on a caloric restricted intake (60% of the AL diet). The administration of BL in Y/AL, O/AL and O/CR animals activated the 35S- labeling of sp 90 which reached a peak at 4 hours. Labeling of sp 90 was significantly greater in Y/AL compared to O/AL, and the incorporation pattern of O/CR was intermediate to Y/AL and O/AL animals. All labeling of sp 90 in each group had disappeared by 10 hours after BL administration. Stress protein 70x (inducible form) in these three animal groups displayed a similar pattern of 35Sincorporation, but the amount of labeling was less than that of sp 90. No labeling of sp 70x remained by 13 hours after BL administration. Phosphorylation ([32P] phosphate incorporation) of sp 90 reached a maximum level at 2 hours in all animals, and 32P- labeling in Y/AL was significantly increased over O/AL and O/CR with an intermediate level found in O/CR animals. The turnover rate (phosphorylation/dephosphorylation) of sp 90 induced by BL was significantly suppressed and temporarily extended in O/AL as compared with O/CR, which implied that CR not only increased incorporation of sp 90, but also enhanced a utilization of the phosphate pool very similar to that seen in Y/AL animals. (Aging Clin. Exp. Res. 6: 121- 131, 1994)

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Authors and Affiliations

  1. Dept. of Health and Human Services, Food and Drug Administration, National Center for Toxicological Research, Division of Genetic Toxicology, DGT/HFT-120, Jefferson, AR, 72079-9502, USA

    James L. Pipkin Jr. Ph.D., W. G. Hinson, R. J. Feuers, L. E. Lyn-Cook, P. H. Duffy, R. Hart & D. A. Casciano

  2. University of Arkansas for Medical Sciences, Little Rock, Arkansas, USA

    James L. Pipkin Jr. Ph.D., R. J. Feuers, E. R. Burns, P. H. Duffy & D. A. Casciano

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  1. James L. Pipkin Jr. Ph.D.
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  2. W. G. Hinson
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Pipkin, J.L., Hinson, W.G., Feuers, R.J. et al. The temporal relationships of synthesis and phosphorylation in stress proteins 70 and 90 in aged caloric restricted rats exposed to bleomycin. Aging Clin Exp Res 6, 121–131 (1994). https://doi.org/10.1007/BF03324225

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