Skip to main content
SpringerLink
Log in

The oxygen transporting capability of neo red cells (NRC) evaluated under total cardiopulmonary bypass

  • Original Article
  • Published:
The Japanese Journal of Thoracic and Cardiovascular Surgery Aims and scope Submit manuscript

Abstract

The oxygen transporting capability of an artificial oxygen carrier NRC was evaluated by employing it as a perfusate for total cardiopulmonary bypass. NRC is a type of liposome encapsulated hemoglobin. It has a particle size of approximately 220 nm, with a hemoglobin concentration of 5.6 g/dl and its P50 is controlled to 45 Torr. Male beagles were used in the experiment. Approximately 80% of the estimated circulatory volume was exchanged with NRC and total cardiopulmonary bypass was initiated. Arterial oxygen tension and carbon dioxide tension were controlled to 400 Torr and 40 Torr respectively. The perfused we heated to 37°C. The rate of flow was altered during the experiment. Oxygen consumption reached a plateau at 9.3 ml/kg/min where oxygen delivery was 14.9 ml/kg/min. At this point the oxygen consumed per gram of hemoglobin from NRC was equivalent to that from dog red blood cells. This indicated that almost an equal amount of oxygen was consumed from NRC in comparison to red blood cells. Regarding oxygen transporting capability, NRC could be considered a candidate for perfusate in cardiopulmonary bypass.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Tomasulo P: Transfusion alternatives. Impact on blood banking worldwide, Winslow RM, Vandegriff KD, Intaglietta M, ed., Blood substituters. Physiological basis of efficacy. Boston, 1995, Birkhauser, p1–19

    Google Scholar 

  2. Tsuchida E: Introduction. Overview and Perspectives, Tsuchida E, ed., Artificial Red Cells, New York, 1995, John Wiley & Sons, p1–20

    Google Scholar 

  3. 19: 1477–1482, 1995

  4. Zuck TF, Riess JG: Current status of injectable oxygen carriers. Crit Rev Clin Lab Sci 31: 295–324, 1994

    Article  PubMed  CAS  Google Scholar 

  5. Chang TMS: Blood substitutes based on modified hemoglobin prepared by encapsulation or crosslinking. An overview. Biomater Artif Cells Immobilization Biotechnol 20: 159–182, 1992

    PubMed  CAS  Google Scholar 

  6. Looker D, Abbott-Brown D, Cozart P, Durfee S, Hoffman S, Mathews AJ, Miller-Roehrich J, Shoemaker S, Trimble S, Fermi G, Komiyama NH, Nagai K, Stetler GL: A human recombinant haemoglobin designed for use as a blood substitutes. Nature 356: 258–260, 1992

    Article  PubMed  CAS  Google Scholar 

  7. Djordjevich L, Miller IF: Synthetic erythrocytes from lipid encapsulated hemoglobin. Exp Hemat 8: 584–592, 1980

    PubMed  CAS  Google Scholar 

  8. Zheng S, Beissinger R, Sherwood RL, McCormic DL, Lasic DD, Martin FJ: Liposome-encapsulated hemoglobin. A red blood cell substitute. J Liposome Res 3: 575–588, 1993

    Article  CAS  Google Scholar 

  9. Kobayashi K, Tsuchida E, Nishide H: Totally synthetic hemes. Their characteristics and oxygen-carrying capacity in dogs, Tsuchida E, ed., Artificial Red Cells, New York, 1995, John Wiley & Sons Ltd, p93–116

    Google Scholar 

  10. 17: 729–732, 1988

  11. 17: 708–711, 1988

  12. (NRC) 20: 626–630, 1991

  13. (NRC) 7: 230–238, 1993

  14. Ogata Y, Goto H, Sakaguchi K, Suzuki M, Ohsaki K, Suzuki K, Saniabadi AR, Kamitani K, Takahashi A: Characteristics of Neo Red Cells, their function and safety. In vivo studies. Art Cells Blood Subs and Immob Biotech 22: 875–881, 1994

    Article  CAS  Google Scholar 

  15. Takahashi A: Characterization of Neo Red Cells (NRCs), their function and safety in vivo tests. Art Cells Blood Subs and Immob Biotech 23: 347–354, 1995

    Article  CAS  Google Scholar 

  16. Izumi Y, Sakai H, Hamada K, Takeoka S, Yamahata T, Kato R, Nishide H, Tsuchida E, Kobayashi K: Physiologic responses to exchange transfusion with hemoglobin vesicles as an artificial oxygen carrier in anesthetized rats. Changes in mean arterial pressure and renal cortical oxygen tension. Crit Care Med 24: 1869–1873, 1996

    Article  PubMed  CAS  Google Scholar 

  17. 7: 422–425, 1978

  18. 15: 1035–1037, 1967

  19. Miller MJ: Tissue oxygenation in clinical medicine. An historical review. Anes Anaig 61: 527–535, 1982

    CAS  Google Scholar 

  20. Kandel G, Aberman A: Mixed venous oxygen saturation. Its role in the assessment of the critically ill patient. Arch Intern Med 143: 1400–1402, 1983

    Article  PubMed  CAS  Google Scholar 

  21. 35: 599–607, 1987

  22. Villar J, Slutsky AS, Hew E, Aberham A: Oxygen transport and oxygen consumption in critically ill patients. Chest 98: 687–692, 1990

    Article  PubMed  CAS  Google Scholar 

  23. Cohen AH, Nast CC: Anderson’s Pathology, Damjanov I, Linder J, ed., St. Louis, 1996, Mosby-Year Book, Inc., p2117–2120

    Google Scholar 

  24. 29: 153–163, 1974

  25. 16: 299–310, 1984

  26. 1991, p159–169

Download references

Author information

Authors and Affiliations

  1. Department of Surgery, School of Medicine, Keio University, Tokyo, Japan

    Yotaro Izumi, Takeshi Yamahata, Ryohei Yozu & Koichi Kobayashi

  2. Department of Pathology, School of Medicine, Keio University, Tokyo, Japan

    Makio Mukai

Authors
  1. Yotaro Izumi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Takeshi Yamahata
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ryohei Yozu
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Koichi Kobayashi
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Makio Mukai
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Izumi, Y., Yamahata, T., Yozu, R. et al. The oxygen transporting capability of neo red cells (NRC) evaluated under total cardiopulmonary bypass. Jpn J Thorac Caridovasc Surg 46, 30–37 (1998). https://doi.org/10.1007/BF03217719

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF03217719

Keywords

Search

Navigation