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Endonuclease restriction of SCAR amplicons SC811 is required to identifyNs-false-positive markers in PVS-susceptible potato cultivars

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Abstract

TheNs gene confers resistance of potato toPotato virus S (PVS). Sixteen German and Dutch potato cultivars, all registered in Poland, were found to be susceptible to PVS infection. However, scoring of the cultivars for the presence of theNs-linked SCAR marker SC811454 revealed additional amplicons with a similar electrophoretic migration rate as that of SC811454, which resulted in ambiguous determination of the genotype at the Ns locus. MboI or FokI treatment of the PCR products allowed to detect theirNs-unspecificity in PVS-susceptible potato cultivars.

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Authors and Affiliations

  1. Plant Breeding and Acclimatization Institute, Platanowa 19, 05-831, Młochów, Poland

    Katarzyna Szajko, Mirosława Chrzanowska, Danuta Strzelczyk-Żyta, Helena Zagórska & Waldemar Marczewski

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  1. Katarzyna Szajko
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  2. Mirosława Chrzanowska
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  3. Danuta Strzelczyk-Żyta
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  4. Helena Zagórska
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  5. Waldemar Marczewski
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Correspondence to Waldemar Marczewski.

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Szajko, K., Chrzanowska, M., Strzelczyk-Żyta, D. et al. Endonuclease restriction of SCAR amplicons SC811 is required to identifyNs-false-positive markers in PVS-susceptible potato cultivars. J Appl Genet 49, 45–47 (2008). https://doi.org/10.1007/BF03195248

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  • DOI: https://doi.org/10.1007/BF03195248

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