Conclusion
Twelve metabolites of T-2 toxin and two metabolites of DAS have been detected by GLC-MS in the perfusate of isolated rat livers. Less than 50% of the administered dose was recovered in the perfusate. However, preliminary results of the analysis of bile show that considerable amounts of T-2 toxin and DAS were excreted mainly as glucuronide conjugates. The concurrence of the data reported here with the results of in vivo studies indicate that the isolaged perfused rat liver system is suitable in studying the metabolism of trichothecence mycotoxins.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Gareis M, Bauer J, Gedek B (1985) Fusarientoxine in Futtermitteln. Nachweis und Vorkommen von Trichothecenen. Tierärztl Prax Suppl 1:8–19.
Szathmáry CI (1983) Trichothecene toxicoses and natural occurrence in Hungary. In: Ueno Y (ed) Developments in Food Science 4: Trichothecenes. Kodansha Ltd, Tokyo, pp 229–250.
Vesonder RF (1983) Natural occurrence in North America. In: Ueno Y (ed) Developments in Food Science 4: Trichothecenes. Kodansha Ltd, Tokyo, pp 210–217.
Yoshizawa T, Mirocha CJ, Behrens JC, Swanson SP (1981) Metabolie fate of T-2 toxin in a lactating cow. Fd Cosmet Toxicol 19:31–39.
Visconti A, Mirocha CJ (1985) Identification of various T-2 toxin metabolites in chicken excreta and tissues. Appl Environ Microbiol 49:1246–1250.
Yoshizawa T, Swanson SP, Mirocha CJ (1980) In vitro metabolism of T-2 toxin in rats. Appl Environ Microbiol 40:901–906.
Yoshizawa T, Sakamoto T, Ayano Y, Mirocha CJ (1982) 3’-hydroxy T-2 and 3’-hydroxy HT-2 toxins: new metabolites of T-2 toxin, a trichothecene mycotoxin, in animals. Agric Biol Chem 46:2613–2615.
Yoshizawa T, Okamoto K, Sakamoto T, Kuwamura K (1985) In vivo metabolism of T-2 toxin, a trichothecene mycotoxin. On the formation of depoxydation products. Proc Jpn Assoc Mycotoxicol 21:9 -12.
Ohta M, Matsumoto H, Ishii K, Ueno Y (1978) Metabolism of trichothecene mycotoxins. II. Substrate specifity of microsomal deacetylation of trichothecenes. J Biochem 84:697–706.
Bauer J, Bollwahn W, Gareis M, Gedek B, Heinritzi K (1985) Kinetic profiles of diacetoxyscirpenol and two of its metabolites in blood serum of pigs. Appl Environ Microbiol 49:842–845.
Staib W, Scholz R (eds) Stoffwechsel der isoliert perfundierten Leber. Springer Verlag, Berlin 1968.
Ertl B (1983) Experimentelle Untersuchungen über die unspezifische Abwehr der Leber gegenüber unterschiedlichen Virusarten mit Hilfe der isoliert perfundierten Rattenleber. Diss Vet Med, Universität München.
Sano A, Asabe Y, Takitani S, Ueno Y (1982) Fluorodensitometric determination of trichothecene mycotoxins with nicotinamide and 2-acetylpyridine on a silica gel layer. J Chromat 235:257–265.
Pawlosky RJ, Mirocha CJ, Yoshizawa T (1984) Reaction products (isomers) of two metabolic derivatives of T-2 toxin (TC-1 and TC-3) when reacted with trifluoroacetic acid anhydride. J Agric Fd Chem 32:1423–1425.
Pawlosky RJ, Mirocha CJ (1984) Structure of a metabolic derivative of T-2 toxin (TC-6) based on mass spectrometry. J Agric Fd Chem 32:1420–1423.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Gareis, M., Ertl, B., Bauer, J. et al. Biotransformation of T-2 toxin and diacetoxyscirpenol in the isolated perfused rat liver. Mycotox Res 1, 77–82 (1985). https://doi.org/10.1007/BF03192007
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF03192007