Abstract
Interspecific and intergeneric hybridizations have been widely used in plant genetics and breeding to construct stocks for genetic analysis and to introduce into crops the desirable traits and genes from their relatives. The intergeneric crosses betweenBrassica juncea (L.) Czern. & Coss.,B. carinata A. Braun andOrychophragmus violaceus (L.) O. E. Schulz were made and the plants produced were subjected to genomicin situ hybridization analysis. The mixoploids from the cross withB. juncea were divided into three groups. The partially fertile mixoploids in the first group (2n = 36–42) mainly contained the somatic cells and pollen mother cells (PMCs) with the 36 chromosomes ofB. juncea and additional chromosomes of O.violaceus. The mixoploids (2n = 30–36) in the second and third groups were morphologically quite similar to the mother plantsB. juncea and showed nearly normal fertility. The plants in the second group produced the majority of PMCs (2n = 36) with their chromosomes paired and segregated normally, but 1–4 pairs of the O.violaceus chromosomes were included in some PMCs. The plants in the third group produced only PMCs with the 36B. juncea chromosomes, which were paired and segregated normally. The mixoploids (2n = 29–34) from the cross withB. carinata produced the majority of PMCs (2n = 34) with normal chromosome pairing and segregation, but some plants had some PMCs with 1–3 pairs of chromosomes fromO. violaceus and other plants had only PMCs with theB. carinata chromosomes. TheBrassica homozygous plants and aneuploids with complete or partial chromosome complements ofBrassica parents and various numbers ofO. violaceus chromosomes were derived from these progeny plants. The results in this study provided the molecular cytogenetic evidence for the separation of parental genomes which was previously proposed to occur in the hybridizations of these two genera.
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Li, Z., Ceccarelli, M., Minelli, S. et al. High efficiency production and genomicin situ hybridization analysis ofBrassica aneuploids and homozygous plants. Sci. China Ser. C.-Life Sci. 46, 104–112 (2003). https://doi.org/10.1007/BF03182690
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DOI: https://doi.org/10.1007/BF03182690