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Development of a new method for simultaneously evaluating mucociliary clearance and pulmonary epithelial permeability in rabbit experiments by means of18FDG, three-dimensional positron emission tomography and rectilinear scan

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Abstract

We tried to simultaneously obtain the elimination constant of mucociliary clearance and the pulmonary epithelial permeability constant after inhalation of 2-[18F]fiuoro-2-deoxy-d-glucose (18FDG) solution by carrying out whole lung positron emission tomography and a rectilinear scan in rabbit experiments. The elimination constant of pulmonary epithelial permeability was obtained from the decrease in the amount of the radioactivity with time in the region of interest (ROI) confined to the lungs, trachea and tracheal cannula in the rectilinear scan. The total elimination constant of the radioactivity in the lungs was obtained from the ROI confined to the lungs in the tomography. The mucociliary clearance rate constant in the lungs was then obtained after subtracting the elimination constant of the pulmonary epithelial permeability from the total elimination constant of the18FDG in the lungs. The mucociliary clearance constant in the trachea was calculated from the residual radioactivity in the trachea and the mucociliary clearance constant in the lungs. The mean pulmonary epithelial permeability constant was 0.0020% min−1 obtained from the rectilinear scan. The mean mucociliary clearance constants of the lungs and the trachea were 0.0006 and 0.025% min−1, respectively. These results indicated that the pulmonary epithelial permeability and mucociliary clearance could be evaluated simultaneously with18FDG by using three-dimensional positron emission tomography and a rectilinear scan.

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References

  1. Miller RF, O’Doherty MJ. Pulmonary nuclear medicine.Eur J Nucl Med 19: 355–368, 1992.

    Article  CAS  PubMed  Google Scholar 

  2. Rippe B, Crone C. Pores and intercellular junctions.In The Lung, Scientific Foundations, Crystal RG, Wesr JB (Ed.-inChief), New York, Raven Press, Ltd., pp. 349–357, 1991.

    Google Scholar 

  3. Bennett WD, Ilowite JS. Dual pathway clearance of99mTc-DTPA from the bronchial mucosa.Am Rev Respir Dis 139: 1132–1138, 1989.

    CAS  PubMed  Google Scholar 

  4. Wangensteen D, Goodman BE. Developmental changes in alveolar epithelial permeability.Am Rev Respir Dis 127 (suppl): S40-S44, 1983.

    CAS  PubMed  Google Scholar 

  5. Schlesinger RB, Naumann B, Chen LC. A system for measurement of radioactive aerosol retention in the lungs of rabbits.Health Physics 42: 77–80, 1982.

    Google Scholar 

  6. Perry WF, Boyd EM. A method for studying expectorant action in animals by direct measurement of the output of respiratory tract fluid.J Pharm Pharmacol 73: 65–77, 1941.

    CAS  Google Scholar 

  7. Kasé Y, Seo H, Oyama Y, Sakata M, Tomoda K, Takahama K, et al. A new method for evaluating mucolytic expectorant activity and its application. I. Methodology.Arzneim-Forsch 32: 368–373, 1982.

    Google Scholar 

  8. Miyata T, Kai H, Saito M, Okano Y, Takahama K, Nakagawa M, et al. Effects of ambroxol on pulmonary surfactant —Analysis of fatty acid composition of phosphatidylcholine in the sputum and normal respiratory tract fluid in rabbits.Folia PharmacolJpn 88: 57–64, 1986.

    Article  CAS  Google Scholar 

  9. Chen LC, Schlesinger RB. Response of the bronchial mucociliary clearance system in rabbits to inhaled sulfite and sulfuric acid aerosols.Toxicol Appl Pharmacol 71: 123–131, 1983.

    Article  CAS  PubMed  Google Scholar 

  10. Saano V, Minker E, Joki S, Virta P, Nuutinen J, Korbonits D. Influence of chinoin-170, a novel antitussive, on the mucociliary activity in respiratory airways of rats, rabbits, guinea-pigs and man.J Pharm Pharmacol 45: 799–802, 1993.

    CAS  PubMed  Google Scholar 

  11. Ohashi Y, Nakai Y. Mucociliary activity in fetal rabbits.Acta Otolaryngol 97: 351–358, 1984.

    Article  CAS  PubMed  Google Scholar 

  12. John J, Wollmer P, Dahlbäck M, Jonson B. Effect of detergent on alveolar particle clearance due to large tidal ventilation.Thorax 49: 147–150, 1994.

    Article  CAS  PubMed  Google Scholar 

  13. Miyata T, Kai H, Furusawa K, Nakamura H, Saito M, Okano Y, et al. Secretomotor and mucolytic effects of mabuterol, a novel bronchodilator.Arch Int Pharmacodyn 288: 147–160, 1987.

    CAS  PubMed  Google Scholar 

  14. Roth Y, Kronenberg J. The effect of prostaglandins E2 and F2a on brain and tracheal ciliary activity.Laryngoscope 104: 856–859, 1994.

    Article  CAS  PubMed  Google Scholar 

  15. Crystal RG. Alveolar Macrophage.In The Lung, Scientific Foundations, Crystal RG, Wesr JB (Ed.-in-Chief), New York, Raven Press, Ltd., pp. 527–538, 1991.

    Google Scholar 

  16. Phelps ME, Hoffman EJ, Selin C, Huang SC, Robinson G, MacDonald N, et al. Investigation of [18F]2-fluoro-2-deoxyglucose for measure of myocardial glucose metabolism.J NuclMed 19: 1311–1319, 1978.

    CAS  Google Scholar 

  17. Gallagher BM, Ansari A, Atkins H, Casella V, Christman DR, Fowler JS, et al. Radiopharmaceuticals XXVII.18F-Labeled 2-deoxy-2-fiuoro-d-glucose as a radiopharmaceutical for measuring regional myocardial glucose metabolismin vivo: Tissue distribution and imaging study in animals.J Nucl Med 18: 990–996, 1977.

    CAS  PubMed  Google Scholar 

  18. Som P, Atkins HL, Bandoypadhyay D, Fowler JS, MacGregor RR, Matsui K, et al. A fluorinated glucose analog, 2-fluoro-2-deoxy-d-glucose (F-18): Nontoxic tracer for rapid tumor detection.J Nucl Med 21: 670–675, 1980.

    CAS  PubMed  Google Scholar 

  19. Schlesinger RB, Driscoll KE. Mucociliary clearance from the lungs of rabbits following single and intermittent exposures to ozone.J Toxicol Environ Health 20: 125–134, 1987.

    Article  CAS  PubMed  Google Scholar 

  20. Schlesinger RB. Comparative irritant potency of inhaled sulfate aerosols—Effects on bronchial mucociliary clearance.Environ Res 34: 268–279, 1984.

    Article  CAS  PubMed  Google Scholar 

  21. Schlesinger RB, Chen L-C, Driscoll KE. Exposure-response relationship of bronchial mucociliary clearance in rabbits following acute inhalations of sulfuric acid mist.Toxicology Letters 22: 249–254, 1984.

    Article  CAS  PubMed  Google Scholar 

  22. Effros RM, Mason GR. Measurements of pulmonary epithelial permeabilityin vivo.Am Rev Respir Dis 127 (suppl): S59-S65, 1983.

    CAS  PubMed  Google Scholar 

  23. Huchon GJ, Montgomery AB, Lipavsky A, Hoeffel JM, Murray JF. Respiratory clearance of aerolized radioactive solutes of varying molecular weight.J Nucl Med 28: 894–902, 1987.

    CAS  PubMed  Google Scholar 

  24. Elliott RB, Edgar BW, Pilcher CC, Quested C, McMaster J: Parenteral absorption of insulin from the lung in diabetic children.Aust Paediatr J 23: 293–297, 1987.

    CAS  PubMed  Google Scholar 

  25. Enna SJ, Shanker LS. Absorption of drugs from the rat lung.Am JPhysiol 223: 1227–1231, 1972.

    CAS  Google Scholar 

  26. Shim C, Williams H Jr. The adequacy of inhalation of aerosol from canister nebulizers.Am J Med 69: 891–894, 1980.

    Article  CAS  PubMed  Google Scholar 

  27. Newhouse MT, Dolovich MB. Control of asthma by aerosols.New Engl J Med 315: 870–874, 1986.

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Pharmacy, Yamagata University Hospital, 2-2-2, Iida-Nishi, 990-9585, Yamagata, Japan

    Fumiyoshi Ojima & Yoshito Nakagawa

  2. Cyclotron and Radioisotope Center, Tohoku University, Japan

    Tatsuo Ido, Masatoshi Itoh, Shoichi Watanuki & Shinya Seo

  3. Department of Radiology and Nuclear Medicine, Akita Research Institute for Brain and Blood Vessels, Japan

    Jun Hatazawa

  4. MECT Corporation, Tokyo

    Hiroshi Shinohara & Takayuki Ishii

  5. Department of Pharmacological Sciences, Faculty of Pharmaceutical Sciences, Kumamoto University, Japan

    Hirofumi Kai, Kazuo Takahama & Takeshi Miyata

Authors
  1. Fumiyoshi Ojima
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  2. Tatsuo Ido
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  3. Jun Hatazawa
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  4. Masatoshi Itoh
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  5. Hiroshi Shinohara
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  6. Shoichi Watanuki
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  7. Shinya Seo
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  8. Hirofumi Kai
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  9. Kazuo Takahama
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  11. Yoshito Nakagawa
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  12. Takeshi Miyata
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Correspondence to Fumiyoshi Ojima.

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Ojima, F., Ido, T., Hatazawa, J. et al. Development of a new method for simultaneously evaluating mucociliary clearance and pulmonary epithelial permeability in rabbit experiments by means of18FDG, three-dimensional positron emission tomography and rectilinear scan. Ann Nucl Med 12, 231–235 (1998). https://doi.org/10.1007/BF03164907

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  • DOI: https://doi.org/10.1007/BF03164907

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